6/19/2015 Biosciences eastern and central Africa International - - PDF document

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Biosciences eastern and central Africa – International Livestock Research Institute (BecA‐ILRI) Hub

Mobilizing biosciences for Africa’s development

BecA‐ILRI Hub Nairobi, Kenya 15‐19 June, 2015

Laboratory Management and Equipment Operations Workshop. Rwanda Agricultural Board. (RAB)-Rubona Station. 15th-19th June-2015.

SAM CHIERA. SAM CHIERA.

BIOL BIOLOGIC ICAL S SAFET FETY CABIN CABINET.

BIOLOGICAL S SAFE FETY C CABINET - INT INTRODUCTION. N. This is an enclosed, ventilated laboratory workspace. Mainly used for safely working with materials contaminated with pathogens (anything that can cause/produce disease). It is usually in a negative pressure for containment purpose.

Ty Types o

• f BSC.

BSC are classified in three classes. They are categorized in three ways of protection. Level of personnel protection. Level of environmental protection Level of product protection.

Types o pes of Classes. sses.

Class I. Class II. Class III.

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Classif ssifications. cations.

Classification Biosafety Level Application Class I 1, 2, 3 low to moderate risk biological agents Class II 1, 2, 3 low to moderate risk biological agents Class III 4 high risk biological agents

Class 1 ss 1.

Class I. cabinets provide personnel protection but no product protection and environmental. this class are either ducted (connected to the building exhaust system) or unducted (recirculating filtered exhaust back into the laboratory).

BSC- BSC-CL CLAS ASS I. S I. Class I ss II

Class II cabinets provide both kinds of protection of the samples and of the environment. makeup air is also HEPA‐filtered (High Efficiency Particulate Air). These are further classified as Type A1,Type A2,Type B1,Type B2. Each type's requirements are defined by NSF International Standard 49.

BSC- BSC-CL CLAS ASS II S II Type A1 A1 (class (class II). II).

The Type A1 cabinet has a minimum inflow velocity

• f 75 ft./min.

The filtered makeup air is divided equally over the work surface at about two to six inches above the work surface. Exhaust is drawn at the bottom of the cabinet where it rises to the top. At the top of the cabinet, 70% of the air recirculates through the supply HEPA filter, the other 30% of air exhausted through the exhaust HEPA filter.

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BSC-CL BSC-CLAS ASS S II T II TYPE PE A I A I Type A 2 (clas A 2 (class II). II).

Has a minimum inflow velocity of 100 ft/min. A negative air pressure plenum surrounds all contaminated plenums that are under positive pressure.

BSC- BSC-CL CLAS ASS S II T II TYPE PE A II A II Type B1 B1 (clas (class II). II).

The Type B1 cabinets have a minimum inflow velocity of 100 ft/min. These cabinets must be hard‐ducted to an exhaust system( In contrast to the type A1 and A2 cabinets, 60% of air from the rear grille is exhausted and only 40% is recirculated).

BSC- BSC-CL CLAS ASS II S II TYPE PE B B I Type B2 B2 (clas (class II). II).

Type B2 cabinets have a minimum inflow velocity of 100 ft/min Type B2 cabinet is expensive to operate because no air is recirculated within. this type is mainly found in such applications as toxicology laboratories. cabinets of these types generally monitor the exhaust flow, shutting off the supply blower and sounding an alarm if the exhaust flow is insufficient.

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BSC- BSC-CL CLAS ASS II S II TYPE PE B B II II Clas Class III. III.

Class III cabinet, generally only installed in maximum containment laboratories. Specifically designed for work with BSL‐4 pathogenic agents, providing maximum protection (A pathogen is anything that causes a disease). The enclosure is gas‐tight. All materials enter and leave through a dunk tank or double‐door autoclave.

Cont. Cont.

Gloves attached to the front prevent direct contact with hazardous materials. These cabinets are sometimes called glove box. Provide a physical barrier between biological agents and personnel.

BSC- BSC-CL CLAS ASS III. S III. BSC- BSC- CLAS CLASS III III

Bi Biol

• logical
• gical Saf

Safety C ty Cabinet T net Testing sting & Validation. ation.

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Certif ification. ication.

A Biological Safety Cabinet MUS MUST be be cer certifi ified. must be performed by experienced, qualified personnel. On-site testing following the recommendations for field testing [NSF/ANSI 49-2011.

When When.

Initial Installation. Moved or Relocated. After a Major Repair. Annually.

Types o pes of tests. sts.

down flow velocity. inflow velocity. airflow smoke patterns. filter leak. light intensity. Vibration. noise level UV light integrity

Ty Types o

• f certificates.

Thank you

Samuel Gakuha Chiera Engineering Technician ILRI Nairobi Kenya Engineering Department s.chiera@cgiar.org Mobilizing biosciences for Africa’s development http://hub.africabiosciences.org