Principles of Microscopy II: Super-resolution Humberto Cabrera - - PowerPoint PPT Presentation

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Principles of Microscopy II: Super-resolution Humberto Cabrera - - PowerPoint PPT Presentation

Jun 04, 2023 9 likes •230 views

Principles of Microscopy II: Super-resolution Humberto Cabrera Venezuelan Institute for Scientific Research International Centre for Theoretical Physics Preparatory School to the Winter College on Optics: Advanced Optical Techniques for Bio-

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Principles of Microscopy II: Super-resolution Humberto Cabrera

Venezuelan Institute for Scientific Research International Centre for Theoretical Physics

Preparatory School to the Winter College on Optics: Advanced Optical Techniques for Bio- imaging

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Diffraction limit of imaging techniques

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The diffraction limit

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The diffraction limit of light

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The diffraction limit

Molecules within the región < 200 nm are not discernible

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STED microscopy

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Experimental setup

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Confocal versus STED microscopy

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Applications of STED microscopy

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STED, RESOLFT microscopy

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STED, RESOLFT microscopy

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Total internal reflection fluorescence (TIRF) microscopy

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Principles of TIRF microscopy

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Experimental setup

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Experimental setup

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Experimental setup

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Experimental setup

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Experimental setup

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Experimental setup

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Applications

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Thanks