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SCHOTT Lighting and Imaging SCHOTT Microscopy www.lambdaphoto.co.uk T: +44 (0)1582 764334 LE-RS/PSb, 24.05.2011 SCHOTT Lighting and Imaging Contents Contents overview general lighting situations illumination with fiber optics

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

SCHOTT Microscopy

www.lambdaphoto.co.uk T: +44 (0)1582 764334

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

  • overview general lighting

situations

  • illumination with fiber optics
  • direct LED illumination

Contents Contents

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Microscope Microscope Types Types

Stereo Microscope Classic (Light) Microscope

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Types Types of Stereo

  • f Stereo Microscopes

Microscopes

„GREENOUGH“-type „CMO“-type (Common Main Objectíve)

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Contrasting of specimen in stereo - microscopy is highly dependent on ... the choice of the right illumination system!!!

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

INCIDENT LIGHTING INCIDENT LIGHTING

  • BRIGHTFIELD
  • blique illumination

(spotlight, lightline)

  • BRIGHTFIELD

homogeneous illumination (ringlight)

  • DARKFIELD illumination
  • polarization
  • fluorescent illumination
  • co-axial illumination
  • near vertical illumination

Overview Overview Illumination Illumination Techniques Techniques

TRANSMITTED LIGHTING TRANSMITTED LIGHTING

  • BRIGHTFIELD illumination
  • DARKFIELD illumination
  • polarization
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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Incident Incident Light, Light, Ringlight Ringlight -

  • Homogeneous

Homogeneous Illumination Illumination

THEOBROMA CACAO, Cacao bloomer

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Incident Incident Light, Light, Gooseneck Gooseneck ( (Shadowing Shadowing) )

EUPHOLUS

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Incident Incident Ringlight Ringlight vs vs Oblique Oblique Light Line Light Line

ringlight corrosion

  • blique light line
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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Incident Incident Light, Light, Darkfield Darkfield Ringlight Ringlight

EURO coin

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Incident Incident Ringlight Ringlight vs vs Darkfield Darkfield Ringlight Ringlight

ringlight ringlight darkfield

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Incident Incident Ringlight, Ringlight, Polarization Polarization

without polarization polarizer analyzer polarization

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Incident Incident Ringlight, Ringlight, Polarization Polarization

without polarization polarizer analyzer polarization

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Incident Incident Fluorescent Fluorescent Lighting Lighting

excitation Cut-off filter without cut-off filter Cracks at solder joint

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Coaxial Coaxial Incident Incident Lighting Lighting

polished stone

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Near Near Vertical Vertical Illumination, Illumination, Inspection Inspection of

  • f Drilled

Drilled Holes Holes

valve

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Backlight Backlight, , Brightfield Brightfield

bat embryo

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Backlight Backlight, , Darkfield Darkfield

section, rat embryo

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Backlight Backlight, , Polarization Polarization

polarizer analyzer Nylon fibre Cotton fibre

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

  • overview general lighting

situations

  • illumination with fiber optics
  • direct LED illumination

Contents Contents

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Illumination Systems Illumination Systems with with fiber fiber optics

  • ptics

KL Series with halogene bulb KL Series with LED light engine

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Major Application Areas Major Application Areas

  • Stereo-Microscopy
  • Medical Technology
  • Laboratory
  • Machine Vision

Material Science (Industrial) Life Science

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Halogene Halogene Light Sources with Fiber Optics Light Sources with Fiber Optics

KL 1500 compact KL 1500 LCD KL 300 KL 2500 LCD

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

LED Light Sources with Fiber Optics LED Light Sources with Fiber Optics

KL 300 LED KL 1500 LED KL 1500 LED plus KL 2500 LED

NEW

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Spectrum of White Spectrum of White LEDs LEDs

Spectrum blue InGaN LED-Chip yellow Phosphor emission combined spectrum

  • f white LED

400 470 550 700

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Emission Spectrum of KL 1500 LED vs. KL 1500 LCD Emission Spectrum of KL 1500 LED vs. KL 1500 LCD

0,0E+00 5,0E+03 1,0E+04 1,5E+04 2,0E+04 2,5E+04 3,0E+04

350 400 450 500 550 600 650 700 750 800

wavelength [nm]

intensity

KL 1500 LED KL 1500 LCD

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

General Advantages of LED Light Sources General Advantages of LED Light Sources

  • white light (daylight, 5600-6000 K)
  • nearly constant color temperature on dimming
  • high reliability of LEDs (50,000 operating hours)
  • reduced „cost of ownership“ (no bulb changing)
  • low energy consumption (~ 20% compared to halogene)
  • wide range input (world wide selling)
  • DC output (no flickering)
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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Major Advantages of LED Light Sources Major Advantages of LED Light Sources

essential reduction in „cost of ownership“

  • LED lifetime 50,000 h

(70% reduced in brightness)

  • no bulb on stock
  • no service or downtimes
  • low energy consumption
  • approx. 80% of energy savings

(40 W vs 200 W)

Cost comparison KL1500 LCD vs KL1500 LED

weeks / year 52 days / week 5 hrs / shift 8 shifts / day 2 hrs / year 4.160 LED lifetime / hours 50.000 LED lifetime / years 12,02 Bulb lifetime / hours 200 Bulb lifetime / months 0,58 LED wattage / Watt 40

  • Hal. wattage / Watt

200 Price of bulb / € 20 Price of kWh Energy / € 0,20 Savings lifetime per year Nr of bulbs 250,0 20,8 Cost of bulbs / € 5.000 416 Energy / kWh 8.000 666 Energy cost / € 1.600 133 Total / € 6.600 549 Cost of lightsource / € 599 Payback time / years 1,09

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Advantages of LED Light Sources with Fiber Optics Advantages of LED Light Sources with Fiber Optics

  • significantly higher light intensities can be

focussed to a small field of view

  • get more light out of given LED technology

(separate housing ⇒

  • more efficient cooling
  • bigger refelectors
  • more sophisticated optics)
  • installed base of light guides can be used

(just change the light source) ⇒ full flexibility for realizing different illumination techniques

  • no bulky light source housing covers view to specimen
  • compact housing somewhere behind the microscope
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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Advantages of LED Light Sources with Fiber Optics Advantages of LED Light Sources with Fiber Optics

LED Ringlight VisiLED S80-55 at 75 mm working distance compared to gooseneck light guide with KL 1500 LED

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

KL 300 LED

LED light source with single LED light engine

  • brightness equivalent to 30 W halogene light sources
  • usable as standalone or mounted at microscope stands
  • fits to all light guides and accessories of KL 300 series
  • wide range power supply with international clip system
  • no cooling fan (free of noise and vibration)
  • continuous electronical dimming 0...100%
  • separate on/off switch
  • DC driven
  • nearly no color temperature shift on dimming

SCHOTT KL Series

Illumination for Stereo Microscopy

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

KL 300 LED

  • verview mounting adapters

mounting at microscope stand using as standalone

SCHOTT KL Series

Illumination for Stereo Microscopy

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

KL 1500 LED KL 1500 LED

LED light source with multiple LED light engine

  • brightness equivalent to 150 W halogene light sources
  • fits to all light guides and accessories of KL 1500 series
  • inexpensive adapters for other systems available
  • slider for color filters
  • wide range power supply with international clip system
  • very low noise cooling fan
  • continuous electronical dimming 0...100%
  • separate on/off switch
  • DC driven
  • nearly no color temperature shift on dimming

SCHOTT KL Series

Illumination for Stereo Microscopy

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

KL 1500 LED plus KL 1500 LED plus

LED light source with multiple LED light engine

  • basic features like KL 1500 LED
  • light setting linear controllable in 0.1%,

1% and 5% steps

  • display, which indicates brightness

setting, status, or error codes

  • remote controllable with PC (via USB),

foot switch or external remote control

  • electronic shutter function
  • flash function

SCHOTT KL Series

Illumination for Stereo Microscopy

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

KL 2500 LED KL 2500 LED

LED light source with multiple LED light engine

  • basic features like KL 1500 LED
  • brightness equivalent to 250 W halogene light

sources (1000 Lumens out of a Ø 9 mm bundle)

  • light setting linear controllable in 0.1%, 1%

and 5% steps

  • display, which indicates brightness setting,

status, or error codes

  • remote controllable with PC (via USB), foot

switch or external remote control

  • electronic shutter function

available since March 2011

SCHOTT KL Series

Illumination for Stereo Microscopy

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Overview LED Cold Light Sources Overview LED Cold Light Sources

PRICE product name gross [EUR] LED voltage rating LED power rating cooling color tempera- ture max. effective light guide bundle Ø

  • max. light

flux (with light guide Ø) light control display dimming down to 0% filter holder remote control- able foot sw itch plug computer interface

KL 3 0 0 LED

260 9 V 3 W con- vection 5600 K 6 mm 50 lm ( 4.5 m m )

  • KL 1500 LED

599 12 V 24.5 W fan 6000 K 9 mm 380 lm ( 8 mm )

  • KL 1500 LED

plus

714 12 V 24.5 W fan 6000 K 9 mm 380 lm ( 8 mm )

  • KL 2 500 LED

998 24 V 27 W fan 5600 K 9 mm 1000 lm ( 9 mm )

  • PRODUCT DATA

TECHNICAL DATA ILLUMINATIONDATA FEATURES

SCHOTT KL Series

Illumination for Stereo Microscopy

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S CHOTT Lighting and Imaging LE-R S / PS b , 24.05.2011

Articulating arm L = 200 mm 2 x M6 thread P/N 158 345 Holder for flexible light guides P/N 158 330 Halogen filter for focusing lens P/N 158 207 Polarizing filter P/N 158 205 Holder for focusing lens P/N 158 341 Focusing lens P/N 158 210 Base 1) Ø = 100 mm 3 x M6 thread P/N 158 340 Gooseneck light guide 1-arm 3.5 mm optic fiber bundle, 500 mm P/N 170 101 Gooseneck light guide 2-arms 3.5 mm optic fiber bundle, 500 mm P/N 170 202 6-point ringlight Øi = 58 mm, flexible length 900 mm P/N 172 501 6-point ringlight Øi = 66 mm, flexible length 900 mm P/N 172 601 Annular ringlights for KL 200, KL 1500 LCD, KL 2500 LCD Øi = 40 mm P/N 157 040 Analyzer M 49 x 0.75, type CZ P/N 158 505 Analyzer M 52 x 1, type LEICA P/N 158 510 Transmitted light stage; insert diameter 84 mm, for flexible light guide up to Ø 5 mm P/N 122 150 Polarizing filter attachment for TL P/N 158 500 Flexible light guide 1-arm 4.5 mm optic fiber bundle, 600 mm P/N 121 101 Flexible light guide 1-arm 4.5 mm optic fiber bundle, 1000 mm P/N 171 101 Flexible light guide 2-arms 4.5 mm optic fiber bundle, 1000 mm P/N 171 202 Combi light guide 2-arms 4.5 mm flexible, 600 mm 3.5 mm gooseneck, 500 mm P/N 120 402 KL 300 LED P/N 120 300 Mounting bracket P/N 120 250 Mounting bracket for column Ø 25 mm P/N 120 225 for column Ø 29 mm P/N 120 229 for column Ø 32 mm P/N 120 232 for column Ø 35 mm P/N 120 235

System Diagram System Diagram KL 300

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

System Diagram KL 1500 / 2500 System Diagram KL 1500 / 2500

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

  • overview general lighting

situations

  • illumination with fiber optics
  • direct LED illumination

Contents Contents

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Direct Direct LED Illumination Systems LED Illumination Systems

VisiLED Series EasyLED Series

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Unique Selling Points of Unique Selling Points of EasyLED EasyLED Series Series

  • low costs for high quality and functionality
  • no separate controller box
  • free of noise and vibrations (no fan)
  • ergonomic operating

(not necessary to remove eyes from eyepieces)

  • all devices separately dimmable
  • world wide plug (clip system)
  • usage of standard extension cables (→ bridge larger distances from

power supply to illuminator)

SCHOTT EasyLED Series

Illumination for Stereo Microscopy

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Illuminance at Free Working Distance (FWD) of Various Ringlights

10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 10 20 30 40 50 60 70 80 90 100 110 120 130

Free Working Distance [mm] Illuminance [klx]

EasyLED Ringlight EasyLED Ringlight Plus Ringlight Competitor 1 Ringlight Competitor 2 Ringlight Competitor 3

SCHOTT EasyLED Series

Illumination for Stereo Microscopy

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Measuring of Illuminance

Measuring Device: Gigahertz VL-3704-2 photometric detector head, Ø 7 mm P-9710-1 optometer read-out device Measure equipment

SCHOTT EasyLED Series

Illumination for Stereo Microscopy

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

System Diagram System Diagram EasyLED EasyLED Series Series

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Unique Features of VisiLED System

small groups of LEDs can be controlled separately by a controller unit or PC

  • define complex geometric lighting scenarios

→ mix brightfield, darkfield, incident- and back lighting,

light coming from different directions

  • scenario can be saved for documentation and recall
  • easy switching between complex lighting scenarios
  • free of noise and vibrations (no fan)
  • extremely high brightness due to 80 LEDs

SCHOTT VisiLED Series

Illumination for Stereo Microscopy

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Illuminance at Free Working Distance (FWD) of Various Ringlights

50 100 150 200 250 300 350 10 20 30 40 50 60 70 80 90 100 110 120 130

Free Working Distance [mm] Illuminance [klx]

VisiLED S80-25 VisiLED S80-55 EasyLED RL EasyLED RL Plus

SCHOTT VisiLED Series

Illumination for Stereo Microscopy

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

VisiLED VisiLED Incident Lighting Incident Lighting

Slim Ringlight

  • 48 white Ø 3 mm LEDs
  • dimensions:
  • assembling (inner) diameter 66 mm
  • outer diameter 82 mm
  • height 30 mm
  • variable working distance ranges possible

by exchange of focus optics rings

  • brightfield:
  • WD 20 – 40 mm
  • WD 40 – 90 mm
  • darkfield:
  • WD 5 – 20 mm

available since March 2011

SCHOTT VisiLED Series

Illumination for Stereo Microscopy

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Illuminance at Free Working Distance (FWD) of Slim Ringlight

10 20 30 40 50 60 70 80 10 20 30 40 50 60 70 80 90 100 110

Free Working Distance [mm] Illuminance [klx] VisiLED Slim RL, f50 VisiLED Slim RL, f25 VisiLED Slim RL, f10

SCHOTT VisiLED Series

Illumination for Stereo Microscopy

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Overview Overview Darkfield Darkfield Illumination Illumination

VisiLED Slim Ringlight VisiLED S40-10D

SCHOTT VisiLED Series

Illumination for Stereo Microscopy

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Overview Overview Darkfield Darkfield Illumination Illumination

VisiLED S80-55 + S40-10D fiber optical darkfield ringlight

SCHOTT VisiLED Series

Illumination for Stereo Microscopy

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

System Diagram System Diagram VisiLED VisiLED Series Series

Slim Ringlight

(focus optics rings f25 and f50 included) P/N 400 150

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Applications Applications VisiLED VisiLED Incident Lighting Incident Lighting

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Example Example: Back End of : Back End of Bullet Bullet

contrast enhancement by segmented brightfield full circle quarter circle notches and scratches better visible

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Example Example: : Pit Pit

brightening of deep shade

darkfield 1/4 circle from behind additional brightfield 1/4 circle from top

+ through mixed light

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Example Example: : Insect Insect Leg Leg

highlighting certain features through segmentation and darkfield brightfield brightfield, quarter circle darkfield complete circle

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Example Example: : Pyrit Pyrit

highlighting certain features through segmentation and rotation from behind from right from front

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Example Example: Cartridge : Cartridge Surface Surface

avoid reflections on cylindrical objects full circle two quarter circles

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Example Example: : Coin Coin

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Example Example: : Bulb Bulb Jacket Jacket

TLS brightfiled illumination complete circle quarter circle

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Example Example: : Synthetic Synthetic Textile Fiber Textile Fiber

TLS brightfiled illumination complete circle quarter circle

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Applications Applications VisiLED VisiLED Transmitted Transmitted Lighting Lighting, ACT Base , ACT Base

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Example Example: : Bubbles Bubbles in in Adhesive Adhesive Film Film

contrast enhancement

complete circle complete circle + diaphragm from behind

through shadowing with moveable „semi- diaphragm „

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Example Example: : Bubbles Bubbles in in Adhesive Adhesive Film Film

darkfield, complete circle

contrast enhancement through shadowing with moveable „semi- diaphragm „

darkfield, complete circle, dimmed backlight

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Example Example: Zebra : Zebra Fish Fish

brightfield ACT base „semi-diaphragm“ in certain position brightfield, complete circle

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SCHOTT Lighting and Imaging LE-RS/PSb, 24.05.2011

Example Example: Zebra : Zebra Fish Fish

darkfield ACT base „semi-diaphragm“ in certain position darkfield, complete circle

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SCHOTT Lighting and Imaging

for your attention !

Distribution in the UK & Ireland Lambda Photometrics Limited Lambda House Batford Mill Harpenden Herts AL5 5BZ United Kingdom E: info@lambdaphoto.co.uk W: www.lambdaphoto.co.uk T: +44 (0)1582 764334 F: +44 (0)1582 712084

Characterisation, Measurement & Analysis