iGEM at William & Mary Caroline Golino (CAMS, 17) John Marken - - PowerPoint PPT Presentation

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iGEM at William & Mary Caroline Golino (CAMS, 17) John Marken - - PowerPoint PPT Presentation

Mar 25, 2024 674 likes •910 views

iGEM at William & Mary Caroline Golino (CAMS, 17) John Marken (Mathematics, 17) Margaret Saha (Biology) The new field of synthetic biology promises to change health care, computer technology, the production of biofuels, and more.

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iGEM at William & Mary

Caroline Golino (CAMS, ’17) John Marken (Mathematics, ‘17) Margaret Saha (Biology)

“The new field of synthetic biology promises to change health care, computer technology, the production of biofuels, and

  • more. Students participating in the International Genetically

Engineered Machine (iGEM) competition are on the front lines

  • f this revolution.” [Cell, 26 Jan. 2017]
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Genes consist of modular parts

Promoter Ribosome Binding Site Protein Coding Region Terminator

Icons: Newcastle iGEM 2010

The design (or redesign) and construction of new biological entities such as enzymes, genetic circuits, and cells.

What is Synthetic Biology?

  • - - - -
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Genes (and their modular parts) can be wired into circuits Environmental Signal

Genetic Circuit Processing Cellular Response

Genetic circuits can be coupled to cellular pathways

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What is iGEM (international Genetically Engineered Machine)?

  • iGEM maintains collection of standardized biological parts
  • iGEM hosts annual competition in synthetic biology
  • Students solve real world problems
  • Build genetically engineered biological systems
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2014

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2014 Summary

Submitted Channelrhodopsin-1 and Channelrhodopsin-2 in the BioBrick backbone to iGEM Registry

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2014 Results

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What is Noise?

Expression Expression Time Time

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Noise Measurement Results

pLlacO-1 pLac λ pR Time

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2015 Results

Justin Knight, iGEM Foundation

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Images adapted from Lestas et al 2010, Nature

Application of modular and additive control

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Images adapted from Lestas et al 2010, Nature

Application of modular and additive control

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Images adapted from Lestas et al 2010, Nature

Application of modular and additive control

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Multi-State Response Input Output

Modifying circuit behavior

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Multi-State Response Input Output Shifting Sensitivity Input

Modifying circuit behavior

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Multi-State Response Input Output Scaling Magnitude Shifting Sensitivity Input Input

Modifying circuit behavior

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Multi-State Response Input Scaling Magnitude Shifting Sensitivity Input Input

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2016 Results

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Broader Impacts of iGEM @ W&M:

  • Ideas for new COLL courses
  • Collaboration with W&M “Maker Movement”
  • Synergy with W&M Engineering and Data Science

initiatives

  • Outreach to local schools
  • Partnerships with industry

The BioMakerSpace in ISC3

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The Future of Synthetic Biology: Engineering a Biological Revolution …

Mali et al. 2013, Science Galanie et al. 2015, Science Din et al. 2016, Nature Park et al. 2016, Science Roybal et al. 2016, Cell Pardee et al. 2016, Cell