William and Mary iGEM 2014 Calcium http://arthritida.com / - PowerPoint PPT Presentation

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William and Mary iGEM 2014 Calcium http://arthritida.com / http://www.coa.edu/stodd/oceanweb/bio2/bio2lectures/Lecture7/sld031.htm

William and Mary iGEM 2014
Calcium http://arthritida.com / http://www.coa.edu/stodd/oceanweb/bio2/bio2lectures/Lecture7/sld031.htm http://www.understandinganimalresearch.org.uk/assets/document/37A5997B-D2A9-B867-D7E46F273A86638A/annual-report-for-20.pdf http://openi.nlm.nih.gov/detailedresult.php?img=2689218_1750-1326-4-20-1&req=4 http://www.lifetechnologies.com/order/catalog/product/T3605
The Calcium Kit: Tools for the In Vivo Sensing and Manipulation of Calcium http://www.mpi.nl/people/dediu-dan/DediuDGFS2014Day2web.pdf
Motivation Video courtesy of Wendy Herbst at College of William and Mary
Road to Synthetic Biology + Image courtesy of Wendy Herbst at College of William and Mary
Rationale http://www.mpi.nl/people/dediu-dan/DediuDGFS2014Day2web.pdf
Project Phases Induction Recording Visualization
Phase I: Induce Ca 2+ Influx Channelrhodopsin: Dimerized, closed state http://www.ebi.ac.uk/pdbe-srv/view/entry/3ug9/quaternary.html
Phase I: Induce Ca 2+ Influx http://www.openoptogenetics.org/index.php?title=Channelrhodopsins
Phase I: Induce Ca 2+ Influx Na + H + Ca 2+
Phase II: Visualize Ca 2+ Presence http://www.lifetechnologies.com/content/dam/LifeTech/migration/en/images/ics- organized/brands/molecular-probes.par.71542.image.400.416.1.g001108-gif.gif
Phase II: Visualize Ca 2+ Presence http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3586699/figure/F3/
Phase III: Record Ca 2+ Spikes Ca 2+ Ca 2+ Ca 2+
Completed: Phase I
ChR1 vs CatCh ChR1 CatCh
Begun: Phase II http://journal.frontiersin.org/Journal/10.3389/fnmol.2013.00002/full
Goal: Phase III http://2010.igem.org/Team:Paris_Liliane_Bettencourt/Project/Memo-cell http://2011.igem.org/Team:Groningen
Future Directions: The Calcium Kit http://archiv.aktuelle-wochenschau.de/2013/w51/woche51.html http://journal.frontiersin.org/Journal/10.3389/fnmol.2013.00002/full http://2010.igem.org/Team:Paris_Liliane_Bettencourt/Project/Memo-cell
Future Directions: W&M iGEM
Acknowledgements Undergraduate Science Education Grant to the College of William & Mary William & Mary Dean of Arts & Sciences Dr. Dennis Manos, Vice Provost for Research and Graduate Studies The Charles Center We would like to thank Dr. Peter Hegemann and Dr. James Collins for providing plasmids
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