William and Mary iGEM 2014 Calcium http://arthritida.com / - - PowerPoint PPT Presentation

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William and Mary iGEM 2014 Calcium http://arthritida.com / - - PowerPoint PPT Presentation

William and Mary iGEM 2014 Calcium http://arthritida.com / http://www.coa.edu/stodd/oceanweb/bio2/bio2lectures/Lecture7/sld031.htm


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William and Mary iGEM 2014

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Calcium

http://www.coa.edu/stodd/oceanweb/bio2/bio2lectures/Lecture7/sld031.htm http://arthritida.com/ http://www.understandinganimalresearch.org.uk/assets/document/37A5997B-D2A9-B867-D7E46F273A86638A/annual-report-for-20.pdf http://openi.nlm.nih.gov/detailedresult.php?img=2689218_1750-1326-4-20-1&req=4 http://www.lifetechnologies.com/order/catalog/product/T3605

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The Calcium Kit:

Tools for the In Vivo Sensing and Manipulation of Calcium

http://www.mpi.nl/people/dediu-dan/DediuDGFS2014Day2web.pdf

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Motivation

Video courtesy of Wendy Herbst at College of William and Mary

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Road to Synthetic Biology

+

Image courtesy of Wendy Herbst at College of William and Mary

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Rationale

http://www.mpi.nl/people/dediu-dan/DediuDGFS2014Day2web.pdf

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Project Phases

Induction Visualization Recording

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Phase I: Induce Ca2+ Influx

Channelrhodopsin: Dimerized, closed state

http://www.ebi.ac.uk/pdbe-srv/view/entry/3ug9/quaternary.html

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Phase I: Induce Ca2+ Influx

http://www.openoptogenetics.org/index.php?title=Channelrhodopsins

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Phase I: Induce Ca2+ Influx

Na+ H+ Ca2+

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Phase II: Visualize Ca2+ Presence

http://www.lifetechnologies.com/content/dam/LifeTech/migration/en/images/ics-

  • rganized/brands/molecular-probes.par.71542.image.400.416.1.g001108-gif.gif
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Phase II: Visualize Ca2+ Presence

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3586699/figure/F3/

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Phase III: Record Ca2+ Spikes

Ca2+ Ca2+ Ca2+

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Completed: Phase I

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ChR1 vs CatCh

CatCh ChR1

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Begun: Phase II

http://journal.frontiersin.org/Journal/10.3389/fnmol.2013.00002/full

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Goal: Phase III

http://2010.igem.org/Team:Paris_Liliane_Bettencourt/Project/Memo-cell http://2011.igem.org/Team:Groningen

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Future Directions: The Calcium Kit

http://archiv.aktuelle-wochenschau.de/2013/w51/woche51.html http://journal.frontiersin.org/Journal/10.3389/fnmol.2013.00002/full http://2010.igem.org/Team:Paris_Liliane_Bettencourt/Project/Memo-cell

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Future Directions: W&M iGEM

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Acknowledgements

Undergraduate Science Education Grant to the College of William & Mary William & Mary Dean of Arts & Sciences

  • Dr. Dennis Manos, Vice Provost for Research and Graduate Studies

The Charles Center

We would like to thank Dr. Peter Hegemann and Dr. James Collins for providing plasmids

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Questions?