TEAM TU-EINDHOVEN MRI AND BACTERIA AS DIAGNOSTIC TOOLS THE ROAD - - PowerPoint PPT Presentation

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TEAM TU-EINDHOVEN MRI AND BACTERIA AS DIAGNOSTIC TOOLS THE ROAD - - PowerPoint PPT Presentation

Oct 24, 2023 38 likes •550 views

TEAM TU-EINDHOVEN MRI AND BACTERIA AS DIAGNOSTIC TOOLS THE ROAD TO OUR SOLUTION MRI scan of latter stages of brain cancer Photograph by Zephyr/Science Photo Library TUMOR IMAGING 2007 National Geographic Society CHEMICAL EXCHANGE

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TEAM TU-EINDHOVEN

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MRI AND BACTERIA AS

DIAGNOSTIC TOOLS

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THE ROAD TO OUR SOLUTION

TUMOR IMAGING

MRI scan of latter stages of brain cancer Photograph by Zephyr/Science Photo Library 2007 National Geographic Society

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CHEMICAL EXCHANGE SATURATION TRANSFER (CEST)

ORGANIC CONTRAST AGENTS

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CHEMICAL EXCHANGE SATURATION TRANSFER (CEST)

ORGANIC CONTRAST AGENTS

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CHEMICAL EXCHANGE SATURATION TRANSFER (CEST)

ORGANIC CONTRAST AGENTS

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CHEMICAL EXCHANGE SATURATION TRANSFER (CEST)

ORGANIC CONTRAST AGENTS

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CHEMICAL EXCHANGE SATURATION TRANSFER (CEST)

ORGANIC CONTRAST AGENTS

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CHEMICAL EXCHANGE SATURATION TRANSFER (CEST)

ORGANIC CONTRAST AGENTS

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THE ROAD TO OUR SOLUTION

BACTERIA BASED CANCER THERAPIES

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Our Solution

TEAM TU-EINDHOVEN

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Our Solution

TEAM TU-EINDHOVEN

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Our Solution

TEAM TU-EINDHOVEN

Tumor mor Region gion (Hypox ypoxic c Environ vironme ment) t)

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TEAM TU-EINDHOVEN

Our Solution

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TEAM TU-EINDHOVEN

The Sensor The Chassis Contrast Agents Safety

Detect the hypoxic environment Generate contrast on an MRI scanner Integrate the sensor and contrast agents Ensure patient safety

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The Sensor

TEAM TU-EINDHOVEN

Safety The Chassis Contrast Agents The Sensor

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TEAM TU-EINDHOVEN

The Sensor

Safety The Chassis Contrast Agents The Sensor

Shift to low

  • xygen concentration
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TEAM TU-EINDHOVEN

The Sensor

Safety The Chassis Contrast Agents The Sensor

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TEAM TU-EINDHOVEN

The Sensor

Safety The Chassis Contrast Agents The Sensor

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TEAM TU-EINDHOVEN

The Sensor

Safety The Chassis Contrast Agents The Sensor

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  • High Lysine Ratio

TEAM TU-EINDHOVEN

Contrast Agents

Define chassis Safety The Chassis Contrast Agents The Sensor

1PJN

  • Practicality
  • Molecular

Dynamics Simulations

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TEAM TU-EINDHOVEN

// 1ETF // 1G70 // 1PJN // Human Protamine 1 // Poly Arginine-Glycine // Poly Arginine-Serine // Poly Threonine-Lysine // Poly Lysine-Serine

Define chassis Safety The Chassis Contrast Agents The Sensor

THE SELECTION

Contrast Agents

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AEROBIC EXPRESSION NATIVE PROTEINS ARE CLEARLY EXPRESSED

TEAM TU-EINDHOVEN

Contrast Agents

Define chassis Safety The Chassis Contrast Agents The Sensor

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TEAM TU-EINDHOVEN

Contrast Agents

Define chassis Safety The Chassis Contrast Agents The Sensor

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TEAM TU-EINDHOVEN

Contrast Agents

Define chassis Safety The Chassis Contrast Agents The Sensor

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THE BIOBRICKS

TEAM TU-EINDHOVEN

The Chassis

19 NEW BIOBRICKS:

  • A FUNCTIONAL ANAEROBIC PROMOTER

(BBa_K1123000)

  • 9 CEST PRODUCING PROTEINS

(BBa_K1123013 – BBa_K1123021)

  • 9 COMPLETE TUMOR VISUALIZATION CONSTRUCTS

(BBa_K1123001 – BBa_K1123009)

Safety The Chassis The Sensor Contrast Agents

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The Safety Locks

TEAM TU-EINDHOVEN

Define chassis Safety The Chassis The Sensor Contrast Agents

AVOIDING AN IMMUNE SYSTEM RESPONSE Outer ter Membrane Membrane Periplas Periplas m Cytop toplas las mic c Membr mbrane ane

Illustration by David S. Goodsell, the Scripps Research Institute.

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The Safety Locks

TEAM TU-EINDHOVEN

THE KILL SWITCH

Define chassis Safety The Chassis The Sensor Contrast Agents

Herpes Simplex Virus Thymidine Kinase- Prodrug Ganciclovir System

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TEAM TU-EINDHOVEN

THE KILL SWITCH MODEL

Define chassis Safety The Chassis The Sensor Contrast Agents

The Safety Locks

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BACTERIA AS DIAGNOSTIC TOOLS:

WILL SOCIETY ACCEPT THE IDEA?

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// Bringing the world of synthetic biology into everybody’s home. // Removing the reservations people have about synthetic biology by removing faulty data. // Collaborating and collecting data across the globe.

TEAM TU-EINDHOVEN

SYNTHETIC FACTS

Human Practices

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TEAM TU-EINDHOVEN

SYNTHETIC FACTS

Human Practices

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SYNTHETIC FACTS

21 Initial Facts To Be Checked. 12 Main Topics. 10 Facts Are Officially Checked, Uploaded And Available.

TEAM TU-EINDHOVEN

Human Practices

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TEAM TU-EINDHOVEN

Human Practices

SyntheticFacts.tue.nl

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FINAL CONCLUSIONS

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TEAM TU-EINDHOVEN

We have established the foundations for a new bacterial application. “Synthetic Facts” will continue to give reliable and unbiased information.

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TEAM PICTURE!!!

Special Thanks To…

// dr. Maarten Merkx // dr. ir. Tom de Greef // dr. ir. Natal van Riel // ir. Stijn Aper // Sagitta Peters MSc. // Industrial Designer Mariana Felix // ir. Stephanie Hectors // prof.dr. Klaas Nicolaij // dr. ir. Koen Pieterse

TEAM TU-EINDHOVEN

Acknowledgements

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TEAM TU-EINDHOVEN

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SUPPLEMENTAL SLIDES

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The Sensor

TEAM TU-EINDHOVEN

Global design of the FNR-single and FNR-tandem promoter Reactions of the FNR-tandem promoter

Safety The Chassis Contrast Agents The Sensor

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TEAM TU-EINDHOVEN

The Sensor

Safety The Chassis Contrast Agents The Sensor

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TEAM TU-EINDHOVEN

The Sensor

Safety The Chassis Contrast Agents The Sensor

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CEST PRINCIPLES

Contrast Agents

TEAM TU-EINDHOVEN

Define chassis Safety The Chassis Contrast Agents The Sensor

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Tumor Targeting Kill Switch Environmental Sensor Detectable by CEST MRI Escherichia Coli

The Chassis

TEAM TU-EINDHOVEN

Define chassis Safety The Chassis Contrast Agents The Sensor

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The Safety Locks

TEAM TU-EINDHOVEN

Define chassis Safety The Chassis The Sensor Contrast Agents

AVOIDING AN IMMUNE SYSTEM RESPONSE

Rough Cytoplasm

Journal of Endotoxin Research, Vol. 7, No. 3 , 2001

Polysacchar ide Surface Layer Outer Membran e Cytoplasm ic Membrane O-Antigen of LPS K Capsule Lipid A of Lipopolysacch aride (LPS) Peripla sm

Smooth Rough

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THE KILL SWITCH MODEL

TEAM TU-EINDHOVEN

Define chassis Safety The Chassis The Sensor Contrast Agents

The Safety Locks

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THE ALTERNATIVE KILL SWITCH

TEAM TU-EINDHOVEN

The Safety Locks

Define chassis Safety The Chassis The Sensor Contrast Agents

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TEAM TU-EINDHOVEN

Modeling Overview

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TEAM TU-EINDHOVEN

Modeling: Decoy Sites

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TEAM TU-EINDHOVEN

Modeling: Integral Model

0 Decoy Sites 25 Decoy Sites 50 Decoy Sites 75 Decoy Sites 100 Decoy Sites 125 Decoy Sites 150 Decoy Sites 175 Decoy Sites 200 Decoy Sites