Organic Compounds in Water and Wastewater Cyanotoxins Occurrence - - PDF document

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CEE 697z

Organic Compounds in Water and Wastewater

Cyanotoxins Occurrence & Chemical Analysis

CEE 697z - Lecture #28

Print version

Lecture #28

Microcystin Occurrence in US

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Anatoxin

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Cylindrospermopsin

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Analysis

From: “Analytical Methods for Cyanotoxin Detection and Impacts on Data Interpretation”, Loftin et al., 2010, USG

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• Fig. 5 Overview of sample

preparation and analytical methods for the detection of cyanotoxins.

Merel, S., Walker, D., Chicana, R., Snyder, S., Baures, E. and Thomas, O. (2013) State of knowledge and concerns on cyanobacterial blooms and

• cyanotoxins. Environment

International 59, 303-327.

Analysis

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Analytical Specificity

From: “Analytical Methods for Cyanotoxin Detection and Impacts on Data Interpretation”, Loftin et al., 2010, USGS

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• xic Cyanobacteria in Water: A guide to their public health consequences,

monitoring and management, Edited by Ingrid Chorus and Jamie Bartram , 1999 WHO

Selectivity & Sensitivity

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Sample preparation scheme for determining total BMAA in cyanobacteria (D3-BMAA internal standard was added only to samples for quantification)

Example: Analysis of BMAA I

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ESI-MS/MS product ion spectra of (a) BMAA and (b) D3-BMAA

Analysis of BMAA II

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WRF Study on Methods

 2007, “Determination and Significance of Emerging Algal

Toxins

 Nicholson et al.

 Analytes

 Anatoxin-a  Saxitoxin (STX) & derivatives (PSTs)  Cylindrospermopsin (CYN)

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Preferred Method

 LC/MS/MS selected  HILIC for highly polar PSTs

 Hydrophilic interaction liquid chromatography

 Prior SPE

 procedure

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Selection of SPE phase

Carbograph is non-porous graphitized carbon black. Its surface contains oxygen complexes forming positively charged chemical heterogeneities on surface offering rapid both primary reversed-phase and secondary anion exchange interaction

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