networks (1 st generation device) Donhee Ham, Harvard University - - PowerPoint PPT Presentation

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networks 1 st generation device
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networks (1 st generation device) Donhee Ham, Harvard University - - PowerPoint PPT Presentation

Mar 30, 2023 125 likes •314 views

CMOS electronics probe inside biological cellular networks (1 st generation device) Donhee Ham, Harvard University Electrogenic cellular networks ~10 11 neurons ~10 9 cardiomyocytes ~10 15 synapses ~10 10 cell-cell connections Dichotomy

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CMOS electronics probe inside biological cellular networks (1st generation device) Donhee Ham, Harvard University

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Electrogenic cellular networks

~1011 neurons ~1015 synapses ~109 cardiomyocytes ~1010 cell-cell connections

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Dichotomy ––– intracellular vs. parallel Patch pipette ––– Intracellular, but not parallel

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Dichotomy ––– intracellular vs. parallel Microelectrode array ––– parallel, but not intracellular

  • M. Ballini et al.,

IEEE JSSC (2014)

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Neuronal network Vertical nanowire array CMOS integrated circuit

CMOS nanoelectrode array ––– Intracellular + parallel

  • Prof. Hongkun Park

(Harvard Chemistry & Physics)

Nature Nanotechnology (2017)

Vertical Nanowires Park lab, Nature Nano. 7, 180 (2012).

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Tianyang Ye (Park lab) Jeffrey Abbott

CMOS nanoelectrode array ––– Intracellular + parallel

Nature Nanotechnology (2017)

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Nano-bio array for neurotechnology (with Prof. Park)

Nature Nanotechnology (2017)

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CMOS IC chip (1024 active site array)

Nature Nanotechnology (2017)

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Vertical nanoelectrodes on the surface + packaging

9 nanoelectrodes per pixel Packaged device Cardiomyocyte tissue in vitro cultured on top Nature Nanotechnology (2017)

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Vertical nanoelectrodes

Nature Nanotechnology (2017)

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Post fabrication steps

Nature Nanotechnology (2017)

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Pixel circuit & electrode characterization

Nature Nanotechnology (2017)

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Single myocyte intracellular recording & stimulation

Nature Nanotechnology (2017)

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Why Now? Scalable Nano-Bio Interfacing

Extracellular recording Intracellular recording

Nature Nanotechnology (2017)

Parallel + intracellular recording from 235 cardiomyocytes

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Parallel + intracellular recording from 235 cardiomyocytes

Nature Nanotechnology (2017)

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1 ×

Nature Nanotechnology (2017)

Parallel + intracellular recording – another example

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1/5×

Nature Nanotechnology (2017)

Drug-screening –– Network-level intracellular investigation

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Tianyang Ye (Park) Jeffrey Abbott Marsela Jorgolli (Park) Ling Qin

  • Prof. Hongkun

Park