Near Real-Time Deconvolution for 3D Fluorescence Microscopy Marc - - PowerPoint PPT Presentation
Near Real-Time Deconvolution for 3D Fluorescence Microscopy Marc Bruce, PhD CEO, Microvolution GPU Accelerated Deconvolution Software After 3-D Deconvolution Other Deconvolution 16 Seconds 10.7 Min Thin Filaments Are Missing Preserves Thin
Microvolution offers faster and more accurate deconvolution
Thin Filaments Are Missing Preserves Thin Filaments: 16 sec
Image Courtesy of Molecular Devices
Other Deconvolution 10.7 Min After 3-D Deconvolution 16 Seconds
Sample Objective
Illumination Dichroic mirror
Emission
intensity light
fluorescence
reduces resolution of small
Derived from Wikipedia, “Light sheet fluorescence microscopy”
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http://www.leica-microsystems.com/science-lab/deconvolution
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Iterative algorithm operating under the assumptions of Poisson- distributed noise
faster than old methods
deconvolution
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Seconds
Old methods GPU accelerated
showed low contrast
post-deconvolution
seen
Miskolci, V., Hodgson, L. & Cox, D. Using Fluorescence Resonance Energy Transfer-Based Biosensors to Probe Rho GTPase Activation During Phagocytosis. Methods in Molecular Biology 1519, 125-43 (2017)
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Tong Zhang and Puifai Santisakultarm, Salk Institute
Be Before 2048 x x 2048 x x 51 Aft fter 3D D De Deconvolution 8.5 .5 sec econds
Image Courtesy of Technical Instruments
Dennis Hughes, MD, PhD, Assoc Prof of Pediatrics, Jared Mortus, Lead Researcher, Laurence Cooper, MD, PhD, Professor of Pediatrics, George McNamara, PhD, Senior Research Scientist, The Children’s Cancer Hospital, MD Anderson Cancer Center, Houston, TX
Wid idefie ield Im Image Be Before 1024 x x 1024 x x 34 Aft fter 3D D De Deconvolution 1.6 .6 sec econds
Derived from Wikipedia, “Light sheet fluorescence microscopy”
Ligh Light Shee eet Im Image Be Before 946 x x 768 x x 321 Aft fter 3D D De Deconvolution 22.5 .5 sec econds
Dong-Yuan Chen in the Bilder lab, UC Berkeley
LLS LLSM Be Before 1167 x x 512 x x 401 Aft fter 3D D De Deconvolution 10.2 .2 sec econds
VisiTech
In Instant SIM IM Im Image Be Before 1446 x x 1131 x x 14 Aft fter 3D D De Deconvolution 4.9 .9 sec econds
Siegel, N., Lupashin, V., Storrie, B. & Brooker, G. High-magnification super-resolution FINCH microscopy using birefringent crystal lens interferometers. Nature Photonics 10, 802-808 (2016)
Aft fter Propagation and 3D D De Deconvolu lution 10 sec econ
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TX1 K2200 970M K40 P5000 GP100