MICROALGAE CULTURE (4) BIO301 Dr Navid Moheimani Prof Michael - - PowerPoint PPT Presentation

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MICROALGAE CULTURE (4) BIO301 Dr Navid Moheimani Prof Michael - - PowerPoint PPT Presentation

Jan 14, 2023 3.47k likes •3.94k views

MICROALGAE CULTURE (4) BIO301 Dr Navid Moheimani Prof Michael Borowitzka Flask Cultures Flask Cultures Carbuoy Tubular Airlift Reactor Commercial-Scale production Systems Open ponds Hutt Lagoon; WA Whyalla; SA Dunaliella &

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MICROALGAE CULTURE (4)

BIO301 Dr Navid Moheimani Prof Michael Borowitzka

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Flask Cultures

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Flask Cultures

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Carbuoy

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Tubular Airlift Reactor

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Commercial-Scale production Systems

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Open ponds

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Hutt Lagoon; WA

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Whyalla; SA

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Dunaliella & β-Carotene

  • β-Carotene formation depends on (a) total

irradiance and (b) salinity

  • Optimum salinity for growth ~ 22% NaCl
  • Optimum salinity for β-Carotene formation

> 30% NaCl

  • Nutrient limitation
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Chlorella production - Taiwan

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Chlorella production - Indonesia

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Rores - Kolar, Karnataka, India

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Spirulina Production Ponds, India

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Earthrise Spirulina plant – Calipatria, California Curtesy – Ahma Belay

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Pleurochrysis carterae productivity – Perth, WA Ash-Free DW Lipid CaCO3

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Tank Culture - Hawaii

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Cascade System – Trebon, Czech Repiblic

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Closed Photobioreactors

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Covered Ponds USA

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Bag culture in Aquaculture Hatchery Bag Culture -

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curtesy – Ephraim Cohen Haematococcus ‘tube’ culture in Israel Bag Culture - Israel

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1000 L pilot-scale BIOCOIL at Murdoch University

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Pilot Biocoil – Luton, UK

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Comercial Haematococcus plant: Israel

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Pilot CO2 bioremediation plant operated by Greenfuel Technologies Corporation, USA at MIT.

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Haematococcus plant

  • perated by Algatech Ltd,

Kibbuz Ketura, Israel

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Alveolar panels

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Capital Cost Running Cost Cell Yield Relia- bility Shallow Ponds *(1) * * ** (2) Raceways *** ** ** ***(2) Cascade System **** ** **** ***(2) Tubular Photobioreactor ****** **** *** **** Fermenter ******* ***** (3) ****** *****

1Depends on land & water cost as very large pond area required; 2The range of species which can be cultured is very limited; 3Heterotrophic culture is VERY much cheaper as no light required.

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Economics Bioreactor Design, Engineering & Process Control Algal Physiology, Biochemistry & Molecular Biology Photosynthetic efficiency, Secondary metabolism, ‘Stress’ tolerance, Strain improvement Light supply, Gas exchange, Turbulence, Temperature control, Materials, Harvesting, Downstream processing New applications, marketing, formulation, packaging

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Isolation & Screening

  • Algae strain result of a

20 year isolation & screening programme

  • Strains isolated from

WA saline water sources and screened for suitability for large scale production

  • Detailed studies on

growth, limits to growth and lipid production of most promising spp

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Ideally can release oil (Botryococcus braunii) Rapid Growth High lipid content Temperature optimum High photosynthetic efficiency Ability to tolerate high irradiances Salinity Shear tolerance Non-sticky Grows in selective environment Tolerate high O2 Heavy and large cells Weak or no cell wall Lipid composition

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Why the Pilbara?

  • Sunshine & Climate
  • Land
  • Water
  • Sources of CO2
  • Infrastructure
  • High local demand for

diesel

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Rio Tinto Power Station Algae Pilot Plant (0.01 km2)

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100,000 bbl algal oil production (~ 10% of Australia’s daily consumption) 6.53 km2

Algae Pilot Plant (0.01 km2)

3.25 km 2 km

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100,000 bbl algal oil production (~ 10% of Australia’s daily consumption) 6.53 km2

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Lipid productivity t.ha-1.y-1 Soybeans 0.44 Safflower 0.77 Sunflower 0.95 Rapeseed 1.18 Oil Palm 5.89 Our Alga in Pilbara, WA ~36.00

Comparison of lipid productivity