MICROALGAE CULTURE (4) MICROALGAE CULTURE (4) BIO301 Prof Michael - PowerPoint PPT Presentation

MICROALGAE CULTURE (4) MICROALGAE CULTURE (4) BIO301 Prof Michael Borowitzka S&C1.48

Limits to Growth • Nutrients – N (as nitrate, ammonia, urea) – P – Fe – Si (diatoms) • Supply of inorganic C (CO 2 /HCO 3 - ) • Mixing (turbulence & shear) • Light (except for heterotrophic culture) • Temperature

Flask Cultures

Flask Cultures

Carbuoy

Tubular Airlift Reactor

Commercial-Scale production Systems

Chlorella production - Taiwan

Chlorella production - Indonesia

Rores - Kolar, Karnataka, India

Spirulina Production Ponds, India

Earthrise Spirulina plant – Calipatria, California Curtesy – Ahma Belay

NatureBeta Dunalialla plant, Eilat, Israel curtesy – Ami Ben Amotz

Ash-Free DW Lipid CaCO 3 Pleurochrysis carterae productivity – Perth, WA

Hutt Lagoon; WA

Whyalla; SA

Dunaliella & β -Carotene • β -Carotene formation depends on (a) total irradiance and (b) salinity • Optimum salinity for growth ~ 22% NaCl • Optimum salinity for β -Carotene formation > 30% NaCl • Nutrient limitation

Tank Culture - Hawaii

Cascade System – Trebon, Czech Repiblic

Covered Ponds USA

Bag culture in Aquaculture Hatchery Bag Culture -

Haematococcus ‘tube’ culture in Israel Bag Culture - Israel curtesy – Ephraim Cohen

1000 L pilot-scale BIOCOIL at Murdoch University

Pilot Biocoil – Luton, UK

Comercial Haematococcus plant: Israel

Photobioreactor Tubular China

Pilot CO 2 bioremediation plant operated by Greenfuel Technologies Corporation, USA at MIT.

Haematococcus plant operated by Algatech Ltd, Kibbuz Ketura, Israel

Alveolar panels

Capital Running Cell Relia- Cost Cost Yield bility Shallow Ponds * (1) ** (2) * * Raceways *** (2) *** ** ** Cascade System *** (2) **** ** **** Tubular Photobioreactor ****** **** *** **** Fermenter ***** (3) ******* ****** ***** 1 Depends on land & water cost as very large pond area required; 2 The range of species which can be cultured is very limited; 3 Heterotrophic culture is VERY much cheaper as no light required.

Algal Physiology, Bioreactor Design, Biochemistry & Engineering & Molecular Process Biology Control Light supply, Gas Photosynthetic efficiency, exchange, Turbulence, Secondary metabolism, Temperature control, ‘Stress’ tolerance, Materials, Harvesting, Strain improvement Downstream processing New applications, Economics marketing, formulation, packaging