MICROALGAE CULTURE (4) MICROALGAE CULTURE (4) BIO301 Prof Michael - - PowerPoint PPT Presentation

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MICROALGAE CULTURE (4) MICROALGAE CULTURE (4) BIO301 Prof Michael - - PowerPoint PPT Presentation

Sep 23, 2022 844 likes •1.2k views

MICROALGAE CULTURE (4) MICROALGAE CULTURE (4) BIO301 Prof Michael Borowitzka S&C1.48 Limits to Growth Nutrients N (as nitrate, ammonia, urea) P Fe Si (diatoms) Supply of inorganic C (CO 2 /HCO 3 - )

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MICROALGAE CULTURE (4) MICROALGAE CULTURE (4)

BIO301 Prof Michael Borowitzka S&C1.48

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Limits to Growth

  • Nutrients

– N (as nitrate, ammonia, urea) – P – Fe – Si (diatoms)

  • Supply of inorganic C (CO2

/HCO3

  • )
  • Mixing (turbulence & shear)
  • Light (except for heterotrophic culture)
  • Temperature
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Flask Cultures

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Flask Cultures

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Carbuoy

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Tubular Airlift Reactor

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Commercial-Scale production Systems

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Chlorella production - Taiwan

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Chlorella production - Indonesia

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Rores - Kolar, Karnataka, India

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Spirulina Production Ponds, India

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Earthrise Spirulina plant – Calipatria, California Curtesy – Ahma Belay

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NatureBeta Dunalialla plant, Eilat, Israel curtesy – Ami Ben Amotz

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Pleurochrysis carterae productivity – Perth, WA Ash-Free DW Lipid CaCO3

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Hutt Lagoon; WA

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Whyalla; SA

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Dunaliella & β-Carotene

  • β-Carotene formation depends on (a) total

irradiance and (b) salinity

  • Optimum salinity for growth ~ 22% NaCl
  • Optimum salinity for β-Carotene formation

> 30% NaCl

  • Nutrient limitation
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Tank Culture - Hawaii

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Cascade System – Trebon, Czech Repiblic

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Covered Ponds USA

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Bag culture in Aquaculture Hatchery Bag Culture -

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curtesy – Ephraim Cohen Haematococcus ‘tube’ culture in Israel Bag Culture - Israel

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1000 L pilot-scale BIOCOIL at Murdoch University

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Pilot Biocoil – Luton, UK

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Comercial Haematococcus plant: Israel

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Tubular Photobioreactor China

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Pilot CO2 bioremediation plant operated by Greenfuel Technologies Corporation, USA at MIT.

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Haematococcus plant

  • perated by Algatech Ltd,

Kibbuz Ketura, Israel

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Alveolar panels

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Capital Cost Running Cost Cell Yield Relia- bility Shallow Ponds *(1) * * ** (2) Raceways *** ** ** ***(2) Cascade System **** ** **** ***(2) Tubular Photobioreactor ****** **** *** **** Fermenter ******* ***** (3) ****** *****

1Depends on land & water cost as very large pond area required; 2The range of species which can be cultured is very limited; 3Heterotrophic culture is VERY much cheaper as no light required.

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Economics Bioreactor Design, Engineering & Process Control Algal Physiology, Biochemistry & Molecular Biology Photosynthetic efficiency, Secondary metabolism, ‘Stress’ tolerance, Strain improvement Light supply, Gas exchange, Turbulence, Temperature control, Materials, Harvesting, Downstream processing New applications, marketing, formulation, packaging