Kingston, Canada Queens Canada iGEM 2011 World Championship - - PowerPoint PPT Presentation

Queens Canada iGEM 2011 World Championship Jamboree Kingston, Canada content


 The Challenge
 Approach & Methods
 Our Solution
 Future Applications
 Accomplishments


the problem

why C. elegans?

• Excellent chemotaxis

mechanism

• Multi-cellular=

multiple processes

• Hardy organism

microinjection

conventional method of BioBrick assembly

1C3 1T3 1A3

kill switch mechanism kill switch mechanism

Target(Gene(

• Characterize(in(C.)elegans((
• Assembly(with(other(enzymes(in(C.)elegans,(

create(full(pathway(

• BioBrick(Registry:(develop(bioremedia8on(

pathways(for(other(chassis((( (

future applications does it work?

transparent fast rate of reproduction

part improvement

• Modify(exis8ng(eCFP(part(
• Test(new(promoters(effec8vely((

applications

Tailings(Pond(

Foreign(GPCR( (

GPCR' Source' Ligand' ADBR2( Mouse( Phenol( Orexin( Human( Toluidine,(4EChlorobenzoic(acid,(( Naphthalene(( OX1R( Mouse( 4EChlorobenzoic(acid( MARM1( Rat( Phenol( NPYR1( Mouse( Naphthalene(

chemotaxis part design- promoter

• Conducted(preferen8al(assay(between(target((

and(control(substances(

chemotactic assay

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results

Part: ODR-7: ADRB2-:UNC-54 (BBa_K631032)

results

! Successfully(expressed(eCFP(in(a(nematode( ! Developed(a(novel(method(of(assembly,(PCR( Liga8on( ! Maintained(balance(in(our(approach(to(the( science(by(considering(human(prac8ces(( (

accomplishments

((

sponsors

Faculty Advisors: Dr. Chin-Sang, Dr. Greer, Dr. Ko, Dr. Bendena, Dr. Ramsay, & Dr. Young. Lab Support: Tony Papanicolaou, Jeff Boudreau and rest of Chin-Sang lab. Past QGEM Team Members: Steve Goldie, Chris Palmer, Geoff Halliday, Harry Zhou and James MacLeod. QGEM 2011: Tony He, Stephanie Zhou, Olivia Margie, Alex Campbell, Allister Smith, David Dai, Sophie Gong, Kevin Chen, Kristina Collavino, Eni Rukaj, Faisal Bakhteyar, Eszter Gereb, Gary Wu, Wei Liu, Anujan Poologaindran. QGEM 2010/2011 Executive Team

acknowledgements

1.(Ahmed(S,(Hodgkin(J.((2000)(MRTE2(checkpoint(protein(is(required(for(germline(immortality(and(telomere(replica8on(in(C.)elegans.(Nature,(403:159E164(( ( 2.(Smelick(C,(Ahmed(S.((2005)(Achieving(immortality(in(the(C.)elegans(germline.(Ageing(Research(Reviews,(4(1):67E82(( ( 3.(Telomere(Shortening(and(Damage((2009)((hQp://www.immortalhumans.com/telomereEshorteningEandEdamage/)( ( 4.(Fire(A,(Xu(S,(Montgomery(MK,(Kostas(SA,(Driver(SE,(Mello(CC.((1998)(Potent(and(specific(gene8c(interference(by(doubleEstranded(RNA(in(Caenorhabdi8s( elegans.(Nature,(391(6669):806E11( ( 5.(Tabara(H,(Grishok(A,(Mello(CC.((1998)(RNAi(in(C.)elegans:(soaking(in(the(genome(sequence.(Science,(282(5388):430E1( ( 6.(Timmons(L,(Fire(A.((1998)(Specific(interference(by(ingested(dsRNA.(Nature,(395(6705):854( ( 7.(Timmons(L.((2006)(Construc8on(of(Plasmids(for(RNA(Interference(and(In(Vitro(Transcrip8on(of(DoubleEStranded(RNA.(Methods(Mol(Biol.(351:109E17.( ( 8.(Simmer(F,(Tijsterman(M,(Parrish(S,(Koushika(SP,(Nonet(ML,(Fire(A,(Ahringer(J,(Plasterk(RHA.((2002)(Loss(of(the(Puta8ve(RNAEDirected(RNA(Polymerase( RRFE3(Makes(C.(elegans(Hypersensi8ve(to(RNAi.(Current(Biology,(12(15):(1317E1319.( ( 9.(Kiyohara(H,(Torigoe(S,(Kaida(N,(Asaki(T,(Iida,(T,(Hayashi(H(and(Takizawa(N.((1994)(Cloning(and(Characteriza8on(of(a(Chromosomal(Gene(Cluster,(pah,(That( Encodes(the(Upper(Pathway(for(Phenanthrene(and(Naphthalene(U8liza8on(by(Pseudomonas(pu8da(OUS82.(Journal(of(Bacteriology,(176:2439E2443.(( ( Graphics'(order(of(appearance)( hQp://s.ngm.com/2009/03/canadianEoilEsands/img/candianEoilEsandsE615.jpg(( hQp://en.wikipedia.org/wiki/GPCR(( hQp://ginkgobioworks.com/support/BioBrick_Assembly_Manual.pdf( hQp://www.ncbi.nlm.nih.gov/pmc/ar8cles/PMC205370/((( hQp://wwwEfgg.eur.nl/ch1/cellbiology/images/jansen1.jpg((

references