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Project supervision
- The research was performed in the Laboratory of
Radiobiology, JINR, under the supervision
- f Dr Polina Kutsalo
for calibration curve creation for potential retrospective dose - - PowerPoint PPT Presentation
for calibration curve creation for potential retrospective dose - - PowerPoint PPT Presentation
Chromosomal analysis for calibration curve creation for potential retrospective dose estimation Adamska Klaudia Pinc Michal Wachowski Marcin Project supervision The research was performed in the Laboratory of Radiobiology, JINR, under
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Table of content:
- Aim of the project
- Background info
- The experiment
- Results
- Conclusion
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The aim of the project:
- Estimation of absorbed dose, based on
chromosome observation
- Creation of a curve basing on radiation-
induced unstable chromosomal aberrations
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Background info:
Chromosome
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Background info:
Normal Chromatid break Chromosome fragment Acentric ring
Centric ring with fragment Translocation
Chromatid exchange Dicentric with fragment Reciprocal exchange
Chromosome aberrations
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Microscopic image of metaphase chromosomes with visible dicentric
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The experiment:
Colcemid 46h
Fixation
48-50h Image visible under light microscope
Culture of Irradiated blood
BrdU 0-24 h
PHA
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The experiment – lymphocytes isolation
Peripheral Blood Mononuclear Cell
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Results (our team’s results)
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Results (control – dr. Kutsalo’s results)
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Conclusion
- The amount and severity of aberration is
certainly dose-dependant
- It is possible to estimate the dose
retrospectively, using the curve created at the basis of samples irradiated with known dose and radiation type
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Acknowledgements:
- Chen et al., 2011. Biological dose estimation for accidental
supra-high dose gamma-ray exposure. Radiation Measurements 46, 837-841.
- Gotoh, 2005. Simple biodosimetry method for use in cases of
high-dose radiation exposure that scores the chromosome number of Giemsa-stained drug-induced prematurely condensed chromosomes (PCC). Int. J. Radiat. Biol., vol 81.
- IAEA, 2011. Cytogenic dosimetry: Applications in Prepardness
for and Response to Radiation Emergencies.
- Khvostunov et al., 2011. Recent experience in applying the
cytogenetic dosimetry assay. Radiation Measurements 46, 832-836.
- Nettelbeck and Rabus, 2011. Nanodosimetry: The missing link
between radiobiology and radiation physics? Radiation Measurements 46, 893-897.
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