A New genera tjon of hy giene moni t oring LuciPac A3 Importr: - - PowerPoint PPT Presentation

A New generatjon of hygiene monitoring LuciPac A3

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Importør: Impex Produkter AS Gamle Drammensvei 107 1363 HØVIK

• Tel. 22 32 77 20

info@impex.no www.impex.no

ATP is the universal energy molecule found in all living things.

What’s ATP（Adenosine TriPhosphate）?

Microorganisms Food Residues

ATP ATP ATP ATP

ATP is an indicator

• f uncleanness and

cleaning efficacy.

ATP ATP ATP ATP ATP ATP

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ATP Rapid Hygiene Monitoring ・Invisible uncleanness can be quantified in “Anytime”, “Anywhere” and by “Anyone” in 30 sec.

• Uncleanness record management
• Enhancing employee’s awareness of hygiene

・Risk for both food poisoning and allergen contamination can be checked at the same time.

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ATP ADP AMP

ATP: Adenosine triphosphate

✓ATP is a molecule found in all living cells and serves as an indicator of overall surface hygiene and cleaning efficacy ✓ATP is easily converted into ADP and/or AMP by biological metabolism or by decomposition at high temperature and pH

ATP Hygiene Monitoring

Decomposition of ATP derivatives

A3 detects ATP+ADP+AMP offering higher sensitivity to find microorganisms and biofilms that others miss. Just as fast and easy to use as conventional tests, but its advanced chemistry produces superior detection to uncover contaminants that you may be missing.

Why A3 assay (ATP+ADP+AMP)?

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• ATP
• ADP
• AMP
• A3 assay (ATP+ADP+AMP)

Storage condition : 80 ºC (176 ºF) Detection :

✓ ATP is easily decomposed into ADP and AMP ✓ A3 assay provides consistent results and high sensitivity

Stability of ATP

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PPi: pyrophosphate PEP: phosphoenolpyruvate PPDK: pyruvate orthophosphate dikinase PK: pyruvate kinase

Oxyluciferin + PPi + CO2 D-luciferin + O2

Mg2+

ATP

Luciferase

AMP

PEP + PPi Pyruvate + Pi

Mg2+

AMP

PPDK

ADP

PK

PEP Pyruvate

Mg2+

Recycling A3 assay by Kikkoman Technology

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Kikkoman 3M Hygiena Neogen Charm

BioControl

Instruments Devices Detection Targets ATP +ADP +AMP

ATP ATP ATP ATP ATP

Players on ATP Hygiene Monitoring Test

✓ All players except for Kikkoman detect ATP alone ✓ A3 is able to detect all ATP derivatives (ATP+ADP+AMP)

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Sausage Pork (Raw)

ATP vs A3 ~Meat and Egg~

A3 is a good index of meat & egg residues

Chicken (Raw)

ATP A3

Bacon Beef (Raw)

ATP A3

1,000 2,000 3,000 4,000

ATP A3

牛肉（生）

10,000 20,000 30,000

ソーセージ

1,000 2,000 3,000 4,000 5,000

ATP A3

鶏ムネ肉

100,000 200,000 300,000 400,000 500,000

ベーコン

ATP A3

500 1,000 1,500 2,000 2,500

豚肉（生）

ATP A3 ATP A3

Whole egg (Raw)

ATP A3

300 600 900 1,200

生卵

Relative Light Units (RLU)

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Shrimp (Raw) Sea bream (Raw)

ATP vs A3 ~Seafood~

A3 is a good index of seafood residues

Tuna (Raw)

ATP A3

Salmon (Raw)

ATP A3 ATP A3 ATP A3 ATP A3

Oyster (Raw)

1,000 2,000 3,000 4,000

ATP A3

マグロ

1,000 2,000 3,000

ATP A3

サーモン生

2,500 5,000 7,500 10,000

ATP A3

鯛生

ATP A3

Tuna (Canned)

10,000 20,000 30,000 40,000 50,000

ツナ缶

10,000 20,000 30,000 40,000 50,000 60,000 70,000

生カキ

Relative Light Units (RLU)

100,000 200,000 300,000 400,000 500,000

エビ 9

Ice Cream Yogurt

ATP vs A3 ~Dairy~

A3 is a good index of dairy residues

Milk (Raw)

ATP A3

Whipped Cream

ATP A3 ATP A3 ATP A3 ATP A3

Cheddar cheese

ATP A3

Camembert cheese

100 200 300 400

ATP A3

牛乳

50 100 150

ATP A3

チェダー

50 100 150 200 ホイップクリーム 10,000 20,000 30,000

ヨーグルト

200 400 600 800

アイス

10,000 20,000 30,000 40,000 50,000 60,000 70,000

チーズ

Relative Light Units (RLU)

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Walnuts Cashew

ATP vs A3 ~Nuts~

A3 is a good index of nuts residues

Peanuts

ATP A3

Almond

ATP A3 ATP A3 ATP A3 ATP A3

Hazel nuts

ATP A3

Pistachio

4,000 8,000 12,000 16,000

ATP A3

ピーナッツ

4,000 8,000 12,000 16,000

ATP A3

アーモンド

4,000 8,000 12,000 16,000

カシュー

1,000 2,000 3,000 4,000

くるみ

4,000 8,000 12,000

ATP A3

ヘーゼル

10,000 20,000 30,000 40,000

ATP A3

ピスタ

Relative Light Units (RLU)

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Tomato Banana

ATP vs A3 ~Fruits & Vegetables~

A3 is a good index of fruits & vegetables residues.

ATP A3 ATP A3 ATP A3

Orange

ATP A3

Sesame

4,000 8,000 12,000 16,000

オレンジ

10,000 20,000 30,000

ATP A3

トマト

Soybeans

ATP A3

1,000 2,000 3,000 4,000 5,000

食パン

Potato chips

ATP A3

4,000 8,000 12,000

ATP A3

バナナ

10,000 20,000 30,000 40,000 50,000

ポテチ

10,000 20,000 30,000

白ごま

Relative Light Units (RLU)

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Chocolate Yeast extract

ATP vs A3 ~Fermented foods & fungi~

A3 is a good index of fermented foods & fungi residues.

Bread

ATP A3

Beer

ATP A3 ATP A3 ATP A3 ATP A3

Wine vinegar

ATP A3

Mushroom

1,000 2,000 3,000 4,000

ビール

1,000 2,000 3,000 4,000 5,000

食パン

25,000 50,000 75,000 100,000

ATP A3

酵母エキス

1,000 2,000 3,000 4,000 5,000

チョコレート

5,000 10,000 15,000 20,000

ATP A3

マッシュ

Relative Light Units (RLU)

100 200 300 400 500

ワイン酢 13

Data shows A3 has higher sensitivity

• n detection of food residue than either Hygiena or 3M

Kikkoman A3 vs. Hygiena vs. 3M in food

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Luminescence Intensity (RLU)

A3 vs. Hygiena vs. 3M in Thai Beer

Data shows A3 has higher sensitivity to beer than either Hygiena or 3M

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Sanitation verification by ATP, ADP, and AMP

Before washing Water washing Hot water washing Detergent washing (1st) Detergent washing (2nd)

200RLU

Detection of ATP alone is not enough for sanitation monitoring 16

ATP A3 Fair Fair Excellent

Microorganism Food residue

Excellent Excellent Excellent

Superiority of A3

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Superiority of A3

ATP A3 Fair Fair Excellent

Food residue Microorganism

Excellent Excellent Excellent

A3 can also detect breeding ground of bacteria

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A3 vs Protein assay ~Peanuts~

1 10 100 1,000 10,000 100,000 1,000,000 0.001 0.01 0.1 1 10 100 発光量 タンパク量 (μg/assay) Relative Light Units (RLU) LOD of Bradford Protein assay ： 0.1 μg

The sensitivity of A3 method for peanuts was equal to or better than that of protein assay.

• ： A3
• ： ATP

Protein (μg/assay)

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Protein (μg/assay)

• ： A3
• ： ATP

1 10 100 1,000 10,000 100,000 1,000,000 0.01 0.1 1 10 100 発光量 タンパク量 (μg/assay)

A3 vs Protein assay ~Shrimp~

Relative Light Units (RLU)

The sensitivity of A3 method for shrimp was better than that of protein assay.

LOD of Bradford Protein assay ： 0.1 μg

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Chemicals in samplers

✓ Unwanted chemicals are contained in many products or on swabs, which can cause trace surface contamination, but A3 uses a dry swab that contains no trace contaminants

Kikkoman 3M Hygiena Neogen Charm BioControl

None (dry swab) Chlorhexidinedi gluconate Humectant (SDS info) Benzethonium Chloride (SDS info) Does not contain any hazardous substances at the concentration limits given in Regulation(EC) No.1272.2008 and OSHA HazCom Standard 29 CFR 1910.1200 (SDS info) No show (but pre-moisten) Biofilm breaking agent (leaflet info)

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Conclusion ✓ ATP is easily decomposed into ADP/AMP Assays that rely on ATP alone can produce false negatives for surface cleanliness ✓ A3 assay (ATP+ADP+AMP) detects more – providing high sensitivity and more accurate sanitation verification ✓ LuciPac A3 is sensitive enough for allergen monitoring programs, incl. nuts and eggs ✓ A3 does not leave trace chemical contamination

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500 1000 1500 2000 2500 3000 3500 4000 4500 ATP A3

Chicken

500 1000 1500 2000 2500 ATP A3

Pork

5000 10000 15000 20000 25000 30000 ATP A3

Sausage

500 1000 1500 2000 2500 3000 3500 4000 ATP A3

Tuna

5000 10000 15000 20000 25000 30000 ATP A3

Shrimp

20000 40000 60000 80000 100000 120000 140000 160000 180000 ATP A3

Squid

10000 20000 30000 40000 50000 60000 ATP A3

Canned tuna

200 400 600 800 1000 1200 ATP A3

Egg

2000 4000 6000 8000 10000 12000 14000 16000 18000 ATP A3

Peanut

5000 10000 15000 20000 25000 ATP A3

Dried mango

Luminescence Intensity (RLU)

ATP and A3 detection in food products

✓ Conventional ATP tests do not detect many food products ✓ High Sensitivity LuciPac A3 shows a high level of detection

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Luminescence Intensity (RLU)

✓ LuciPac A3 is more sensitive in beverage detection

ATP and A3 detection in beverages

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