Z. Aspridou , M. Kakagianni, D. Dimakopoulou- Papazoglou, K. - - PowerPoint PPT Presentation

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Z. Aspridou , M. Kakagianni, D. Dimakopoulou- Papazoglou, K. - - PowerPoint PPT Presentation

Sep 25, 2022 19 likes •221 views

Aristotle University of Thessaloniki Faculty of Agriculture, Forestry and Natural Environment School of Agriculture Laboratory of Food Microbiology and Hygiene Z. Aspridou , M. Kakagianni, D. Dimakopoulou- Papazoglou, K. Koutsoumanis 1 st

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1st Symposium on Space Educational Activities 9-12 December, Padova

Aristotle University of Thessaloniki Faculty of Agriculture, Forestry and Natural Environment School of Agriculture Laboratory of Food Microbiology and Hygiene

  • Z. Aspridou, M. Kakagianni, D. Dimakopoulou-

Papazoglou, K. Koutsoumanis

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“Spin Your Thesis 2015” ESA campaign Microbiology and Hygiene Team (MAH)

MAH Team Spin Your Thesis 2015

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  • Lab. Food Microbiology and

Hygiene Aristotle University of Thessaloniki

  • Dep. Food Science and Technology

MAH Team Spin Your Thesis 2015

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Microbiology and Hygiene Team (MAH)

Scientific Interests

  • Microbiological quality and safety of

fresh and processed food products

  • Predictive microbiology
  • Quantitative microbial risk assessment

Growth Inactivation Biofilms Growth limits

Resistance Motility

Hyper -gravity

MAH team proposal

MAH Team Spin Your Thesis 2015

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The effect of hyper-gravity on i) the heat resistance of vegetative mesophilic cells and spores of thermophilic bacteria and ii) the microbial swimming motility Microbial resistance Heat D value Microorganism specific Microbial swimming motility Microorganism specific Microscopic examination Various types of motility

  • Invasion and virulence of the pathogens
  • Sensitivity to other stresses
  • Biofilm formation
  • Cross protection phenomena
  • Dispersal and colonization of niches
  • Competition phenomena
  • Virulence factor
  • Biofilm formation

MAH Team Spin Your Thesis 2015

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The effect of hyper-gravity on i) the heat resistance of vegetative mesophilic cells and spores of thermophilic bacteria and ii) the microbial swimming motility Microbial resistance Heat D value Microorganism specific

MAH Team Spin Your Thesis 2015

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The effect of hyper-gravity on i) the heat resistance of vegetative mesophilic cells and spores of thermophilic bacteria and ii) the microbial swimming motility

MAH Team Spin Your Thesis 2015

Vegetative cells Spores of thermophiles

1 2 3 4 5 6 7 8 9 10 10 20 30 40 50 60 70

Log CFU/ml Time (min)

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The effect of hyper-gravity on i) the heat resistance of vegetative mesophilic cells and spores of thermophilic bacteria and ii) the microbial swimming motility Microbial swimming motility Microorganism specific Microscopic examination Various types of motility

  • Dispersal and colonization of niches
  • Competition phenomena
  • Virulence factor
  • Biofilm formation

MAH Team Spin Your Thesis 2015

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The effect of hyper-gravity on i) the heat resistance of vegetative mesophilic cells and spores of thermophilic bacteria and ii) the microbial swimming motility

MAH Team Spin Your Thesis 2015

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Objective 1: Heat resistance

Salmonella enterica ser. Agona Geobacillus stearothermophilus

Type of microorganism Temperature Gravity Mesophilic (planktonic) 57 and 65°C 10xg, 20xg Thermophilic (spores) 121 °C 20xg

Survival data

Initial population: N0 Final population: Nresidual

MAH Team Spin Your Thesis 2015

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Objective 2: Motility

Pseudomonas fluorescens

Type of microorganism Temperature Gravity Mesophilic (planktonic) ambient 1-20xg

Time lapse videos Cells’ speed, displacement distributions

MAH Team Spin Your Thesis 2015

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MAH Team Spin Your Thesis 2015

Objective 1

Need for consumables Shipment Proper storage Need for equipment Time limitation

Objective 2

Experimental set up Check of the system functionality Sample preparation inoculum and integrity Time limitation Organization Collaboration

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Objective 1: Heat resistance

MAH Team Spin Your Thesis 2015

Difference in the survival under various g levels Different behavior of the survivors Sequential exposure - Gravity to Heat stress

Low to moderate hyper-gravity levels as a stress

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Objective 2: Motility

MAH Team Spin Your Thesis 2015

1g 2g

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Objective 2: Motility

MAH Team Spin Your Thesis 2015

8g 2g sequential

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Objective 2: Motility ImageProPlus

MAH Team Spin Your Thesis 2015

Speed Direction Step Tumbling Runs Energy

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Low to moderate levels of hyper-gravity

  • Stress for the microorganisms
  • Microbial response mechanisms
  • Microbial energy
  • Explanation of microbial behavior
  • Design new processes (Disinfection)
  • Selection of microorganisms-

Technological and functional properties

  • Terrestrial and extraterrestrial application

MAH Team Spin Your Thesis 2015

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MAH Team – Spin Your Thesis 2015

MAH Team Spin Your Thesis 2015

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European Space Agency Education Office and Aristotle University of Thessaloniki for their support European Space Agency Education Office for the scholarship Members of the ESA LDC and LIS laboratory for their help Acknowledgements

MAH Team Spin Your Thesis 2015

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1st Symposium on Space Educational Activities 9-12 December, Padova

Aristotle University of Thessaloniki Faculty of Agriculture, Forestry and Natural Environment School of Agriculture Laboratory of Food Microbiology and Hygiene

  • Z. Aspridou, M. Kakagianni, D. Dimakopoulou- Papazoglou, K.

Koutsoumanis

Thank you for your attention