[PPT] - Compact Dry LS Fe Featu ture Compact Dry L LS Com ompact Dry PowerPoint Presentation

SLIDE 1

Detection of Listeria spp.

Compact Dry LS

SLIDE 2

Com

mpact Dry LS

S is s a si simplified m medium t to

identify

List steria sp

spp. by the c

com

mbination
n of
f se

selective age gents s and chrom

mog
genic su

subst strates. s.

Sample : food and environmental s sample pH : 7.2±0.2 incubation : at 35 or 37℃±1℃, 24hr incubation, if necessary, additional 24hr colony color: light blue / blue colonies storage : one year after manufacturing at Room temperture patent : pending

Fe Featu ture

Compact Dry L LS

SLIDE 3

An Any foo

od,

, beverage ge and water is s pos

ssi

sible as s sa sample.

Sample : S Solid food Liquid food Raw m material Animal feed Be Beverage Wa Water Environmental test ( (swab)

Id Identification

n and e

enumeration

n
f
f List

steria sp spp. Prod

duced by IS

ISO 9001 regi gist stered factor

ry

Be Benefi fit

Compact Dry L LS

SLIDE 4

Exa xamp mple le of

f natu

tura rall lly con

nta

tami minati tion

n

Listeri ria a spp. forms light blue/blu lue e colonies s on the Compact t Dry LS. S. *identi tify fy Listeria spp. only, NOT identify fy L. monocyt ytoge

gene

nes. s.

↑ Close-up ↓ *photos : The samples are commercial fish sausages from two different companies.

Compact Dry L LS

SLIDE 5

Pr Proc

ced

edur ure

(applied ed with ISO SO11290-2) 2)

Compact Dry L LS

Light blue/blue colonies

Resuscitation for 1hr at 20℃ BPW

#05131 Buffered Peptone Water “Nissui” 300g

Sampling from surfaces using swabs Test portion (food and liquid)

Diluent for the preparation

f the initial suspension

Compact Dry LS at 35 or 37℃ for 24hr

Identification and enumeration of Listeria spp.

Confirmation of Listeria spp. :

inoculation on TSYEA and incubation for 16 – 24 hr at 35C or 37C;
catalase reaction;
Gram staining;
motility test (if necessary).

Confirmation of Listeria monocytogenes :

haemolysis test;
carbohydrate utilization;
CAMP test.

Resuscitation for 1hr at 20℃

SLIDE 6

Se Sele lecti tivit ity of

f se

sele lecte ted d st strain ins

Inoculation of 107-109cfu/mL, incubation : 37degreeC, 24hr (+:growth,-:no-growth, cell color:colony color, number: CFU)

strain（more than two） SCD CD LS Oxford ALOA strain（more than two） SCD CD LS Oxford ALOA logCFU /mL

Colony color logCFU/mL Colony color logCFU/mL Colony color logCFU/mL

logCFU /mL

Colony color logCFU/mL Colony color logCFU/mL Colony color logCFU/mL

L. monocytogenes （8）

8.5 ＋ 9.1 ＋ 9.2 ＋ 9.0

S. xylosus

6.6 －－＋ >5.0

L. grayi （2）

8.5 ＋ 8.8 ＋ 8.5 ＋ 8.0

S. thermophilus

7.6 －－－

L. innocua （2）

9.4 ＋ 9.4 ＋ 9.4 ＋ 9.4

C. albicans （2）

6.6 －＋ >6.0 －

L. ivanovii [slow growth]

9.4 ＋ 9.0 ＋ 8.8 ＋ 9.3

S. cerevisiae

7.0 －－－

L. welshimeri （2）

9.1 ＋ 9.0 ＋ 8.9 ＋ 9.2

A. hydrophila

8.5 －－－

B. cereus

5.7 －－－

A. denitrificans

8.5 －－－

B. licheniformis

7.1 －＊ >5.0 ＋ >3.0 ＋ >5.0

A. xylosoxidans

7.6 ＋ >7.0 ＋ >7.0 －

B. subtilis

6.6 －－－

A. faecalis

8.7 －－－

C. minutissimum

7.1 －－－

C. amalonaticus

8.8 －－－

C. renale

7.7 －－－

C. freundii

8.5 －－－

C. xerosis

7.5 －－－

C. koseri

8.4 －－－

E. avium

7.5 －＊ >6.0 －－

E. aerogenes

8.1 －－－

E. durans

7.4 －－－

E. amnigenus

8.1 －－－

E. casseliflavus

7.5 －＊ >6.0 －－

E. cloacae

8.6 －－－

E. faecalis （2）

8.5 －＊ >7.0 －－

E. intermedius （2）

8.6 －－－

E. faecium

8.5 －－－

E. sakazakii

7.7 －－－

E. gallinarum

6.8 －－－

E. blattae

8.3 －－－

E. hirae

7.7 －＊ >6.0 －－

E. coli （4）

8.4 －－－

E. mundtii

7.9 －＊ >7.0 －－

E. coli O157（3）

8.6 －－－

E. raffinosus

7.0 －＊ >6.0 －－

E. fergusonii （2）

8.0 －－－

L. lactis

6.7 －－－

E. hermannii

8.3 －－－

L. citreum

8.1 －－－

H. alvei

8.5 －－－

L. mesenteroides

8.0 －－－

K. oxytoca

8.5 －－－

M. luteus

7.5 －－－

K. ozaenae

8.6 －－－

P. acidilactici

7.9 －－－

K. pneumoniae

8.4 －－－

S. aureus （5）

7.8 －－＋ >6.0

K. ascorbata

7.5 －－－

S. aureus MRSA

8.0 ＋ >5.0 ＋ >5.0 ＋ >6.0

K. cryocrescens

7.5 －－－

S. auricularis

6.6 －－＋ >4.0

M. morganii

8.7 －－－

S. capitis

8.2 －－－

P. mirabilis

8.5 ＋ >7.0 －－

S. epidermidis （2）

7.3 －－－

P. vulgaris

8.7 －－－

S. haemolyticus

6.5 －－－

P. aeruginosa （4）

8.5 ＋ >7.0 －－

S. hominis

7.3 －－－

P. putida

7.9 －－－

S. intermedius

7.5 －－＋ >5.0

R. aquatilis

7.9 －－－

S. lentus

7.3 －－－

S. Typhimurium

8.8 －－－

S. saprophyticus

7.4 －＋ >5.0 ＋ >4.0

S. Choleraesuis

8.8 －－－

S. sciuri

7.5 －－＋ >5.0

S. fonticola

7.3 －－－

S. simulans

7.8 －－－

S. liquefaciens

8.0 －－－

S. warneri

7.5 －－－

S. marcescens （2）

7.8 ＋ >7.0 －－ *:The plate color is changed, no growth

Compact Dry L LS

SLIDE 7

at 37 degree C, 24hr incubation

（Log ： LogCFU/g ） food L.monocytog

genes

inoc

culation

ion (10x/g) CFU/g food L.monocytog

genes

inoc

culation

ion(1 (10x/g) CFU/g CD LS Oxfo ford rd ALOA CD LS Oxfo ford rd ALOA Natural ral cheese 1.0 85 85 50 50 100 barbecued pork 2.0 200 150 200 2.0 240 450 250 3.0 1675 1550 1650 3.0 3500 3450 3200 4.0 14150 12000 11750 cheese 1.0 170 150 150 Sausage (1) 1.0 120 50 50 100 2.0 175 150 300 2.0 220 200 200 3.0 1035 1100 1000 3.0 4950 4350 6150 Italian cheese 2.0 85 85 100 100 Sausage (2) 1.0 115 100 100 3.0 1070 1700 1450 2.0 150 150 200 4.0 9300 7000 5250 Cooked d young bamboo 2.0 120 100 150 Chame memb mbert rt cheese 2.0 305 450 50 50 3.0 790 950 1200 3.0 1610 1950 1900 Salted cod roe 1.0 90 90 100 100 4.0 15950 14850 17200 2.0 135 250 300 Smoked cheese (1) 1.0 125 50 50 50 50 3.0 3500 2850 4000 2.0 140 100 100 Cod roe in soy sauce 2.0 110 100 100 3.0 805 600 800 3.0 185 100 100 Smoked cheese (2) 2.0 105 100 150 Vinegare ared jellyfi yfish 1.0 90 90 50 50 50 50 2.0 125 100 50 50 2.0 165 200 250 Cheese and salami mi 1.0 130 200 150 3.0 745 950 1850 2.0 300 350 450 Drie ied young sardin ines 1.0 105 50 50 50 50 Fish sausage 3.0 935 1050 1050 2.0 145 100 300 4.0 10650 9150 9750 3.0 1745 2300 2050 4.0 8850 10050 9550 whit itebait bait 2.0 170 100 150 sala lami mi 1.0 100 100 50 50 3.0 1995 1700 1950 2.0 135 100 250 4.0 25800 24250 25350 3.0 3300 1450 2300 Salmon

n flake

2.0 180 100 250 Sliced ham (1) 1.0 100 50 50 100 3.0 1450 850 1400 2.0 730 1000 700 4.0 16800 12550 14200 3.0 11050 10250 10800 Mixed d salad 1.0 130 50 50 50 50 Sliced ham (2) 2.0 220 50 50 250 2.0 130 50 50 50 50 Raw ham 1.0 135 100 100 3.0 465 500 400 2.0 310 200 400 Vegetable ble 1.0 400 150 200 3.0 1510 2050 2300 2.0 545 450 250 Roast ham 2.0 225 100 100 3.0 2105 2900 2950 3.0 2020 2100 1900 Macaron roni salad 3.0 135 100 200 4.0 22600 21500 21650 Black soybean 2.0 180 50 50 100

Compact Dry L LS

Inoc

cula

late ted pack k of

f Lis

iste teri ria mo monoc

cyto

toge genes

SLIDE 8

y = 0.893x + 0.3695 r = 0.973 n = 70 1,00 1,50 2,00 2,50 3,00 3,50 4,00 4,50 5,00 1,00 2,00 3,00 4,00 5,00 Compact Dry LS (LogCFU/g) ISO11290-2 (ALOA agar)(LogCFU/g)

Compact Dry LS vs. ISO11290-2 (ALOA agar)

Co Corr rrel elat atio ion n Dat ata

Compact Dry L LS

SLIDE 9

y = 0.9074x + 0.28 r = 0.965 n = 70 1,00 1,50 2,00 2,50 3,00 3,50 4,00 4,50 5,00 1,00 2,00 3,00 4,00 5,00 Compact Dry LS (LogCFU/g) ISO11290-2 (Oxford agar)(LogCFU/g)

Compact Dry LS vs. ISO11290-2 (Oxford agar)

Co Corr rrel elat atio ion n Dat ata a (2 (2)

Compact Dry L LS