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Accurate Computer-assisted Cell Culture using Ultrasounds

Yoa

• ann PAG

AGEAUD D – M2BI

13/12/2016

1838 : P.F. Verhulst - 1er Model Croissance Cellulaire 

𝑒𝑂 𝑒𝑢 = 𝑠𝑂

1951 : G. Gey - Cellule HeLa 2007 : 1ère culture cellulaire 3D par ultrasons 2008 : 1ère culture cellulaire 3D par lévitation magnétique 2009 : 1ère culture cellulaire 3D par impression

Histoire

Rendements trop aléatoire Nécrose rapide des cellules (Culture 3D) Contaminations Erreurs de Manipulation (Humaine)

Solution :

Automatiser et Standardiser les Protocoles !

Problématiques

Les Ondes Stationnaires (1975)

Les Ultrasons : 2 Approches

Le Vortex Acoustique (2016)

Transversale  Longitudinale :

Buckminster Füller (1975)

 Organisation cellulaire  Perméable aux Ultrasons

Matrice Isotrope de Vecteurs (IVM)

Modèle 3D d’une IVM en PCL

50 µm

Le Robot

Panneaux d’Emetteurs Ultrasons (x4) Cartouche de Cellules Pinces Acoustiques (x10) Ecran (Interface) Caméras - Microscopes (x2)

Le Logiciel : Workflow

Programme de Manipulation des Pinces et des Panneaux Entrée : Tableau Coordonnées 3D Cellules, Matrice et Nœuds de pression Sortie : Panneaux et Pinces puis Caméras-Microscopes Pinces Acoustiques Panneaux d’émetteurs ultrasonores Caméras- Microscopes Interface Machine Entrée : DOI ou Protocole + Lancement du Protocole Programme de Text Mining Entrée : Protocole de Springer Protocols Sortie : Paramètres du protocole + Paramètres ultrasons Programme d’Imagerie & Contrôle Qualité Entrée : Lancement Protocole + Piles d’Images Sortie : Tableaux Coordonnées 3D + Scores Qualité + Caméras

Equipes de Recherches

Equipe 1 – « Acoustique et manipulation de cellule unique »

• Pince Acoustique  Cellule Unique dans IVM

Equipe 2 – « Bioinformatiques et culture cellulaire assistée »

• Développement des Programmes + Conception du Robot

Equipe 3 – « Biophysique des ultrasons »

• Effets des Ultrasons sur les Cellules

Calendrier Prévisionnel

12 Trimestres (36 mois)

Equipes Phases

1 2 3 4 5 6 7 8 9 10 11 12 1 1 3 2 2 3 4

Budget Prévisionnel

Frais de fonctionnement

Administratif Diverse (Salaires, Charges, Sécurité, Impôts)

250 000 €

Tests et Prototypage

60 000 €

Brevetage

6 000 € Frais d’équipement

Matériel d’Acoustique

40 000 €

Matériel Informatique

30 000 €

Matériel de Biologie (Wet-Lab)

60 000 € Montant Total 446 000 €

MERCI POUR VOTRE ATTENTION !

Diapositives de Réponses aux Questions

Classification des modèles non-linéaires utilisés en culture cellulaire.

Al-Rubeai, Animal Cell Culture, 10:265.

Atala, Essentials of 3d Biofabrication and Translation, chap. 13.

Diagramme d’utilisation des polymères naturels (rouge) et synthétiques (bleu), dans le domaine de l’impression 3D Biologique.

Ochiai, Takayuki, and Rekimoto, “Three-Dimensional Mid-Air Acoustic Manipulation by Ultrasonic Phased Arrays.”

Baresch, Thomas, and Marchiano, “Observation of a Single-Beam Gradient Force Acoustical Trap for Elastic Particles.”

Bazou et al., “Long-Term Viability and Proliferation of Alginate-Encapsulated 3-D HepG2 Aggregates Formed in an Ultrasound Trap.”

3D bioplotter system. The plotting material is dispensed into a liquid medium layer by layer.

Atala, Essentials of 3d Biofabrication and Translation, p. 158

REFERENCES

Simone Gilgenkrantz, “Regard Sur Soixante Années de Culture de Cellules HeLa,” Histoire Des Sciences Médicales XLVIII, no. 1 (2014). Ian A. Cree, ed., Cancer Cell Culture, vol. 731, Methods in Molecular Biology (Totowa, NJ: Humana Press, 2011), http://link.springer.com/10.1007/978-1-61779-080-5. Kursad Turksen, ed., Bioreactors in Stem Cell Biology, vol. 1502, Methods in Molecular Biology (New York, NY: Springer New York, 2016), http://link.springer.com/10.1007/978-1-4939-6478-9. Scott H. Randell and M. Leslie Fulcher, eds., Epithelial Cell Culture Protocols, 2nd ed, Methods in Molecular Biology 945 (New York: Humana Press ; Springer, 2012). Anthony Atala, Essentials of 3d Biofabrication and Translation (Boston, MA: Elsevier, 2015). Diego Baresch, Jean-Louis Thomas, and Régis Marchiano, “Observation of a Single-Beam Gradient Force Acoustical Trap for Elastic Particles: Acoustical Tweezers,” Physical Review Letters 116,

• no. 2 (January 11, 2016), doi:10.1103/PhysRevLett.116.024301.

Diego Baresch, “Pince Acoustique: Piégeage et Manipulation D’un Objet Par Pression de Radiation D’une Onde Progressive” (Paris 6, 2014), http://www.theses.fr/2014PA066542. Yoichi Ochiai, Hoshi Takayuki, and Jun Rekimoto, “Three-Dimensional Mid-Air Acoustic Manipulation by Ultrasonic Phased Arrays,” arXiv.org, n.d.

• D. Bazou et al., “Long-Term Viability and Proliferation of Alginate-Encapsulated 3-D HepG2 Aggregates Formed in an Ultrasound Trap,” Toxicology in Vitro 22, no. 5 (August 2008): 1321–31,

doi:10.1016/j.tiv.2008.03.014. Mohamed Al-Rubeai, ed., Animal Cell Culture, vol. 9, Cell Engineering (Cham: Springer International Publishing, 2015), p. 297, http://link.springer.com/10.1007/978-3-319-10320-4. Thomas Robert Malthus, Parallel Chapters from the First and Second Editions of an Essay on the Principle of Population (Macmillan, 1909). Turksen, Bioreactors in Stem Cell Biology. Atala, Essentials of 3d Biofabrication and Translation, 6. Al-Rubeai, Animal Cell Culture, 10:265. Atala, Essentials of 3d Biofabrication and Translation, chap. 13.

Glauco R. Souza et al., “Three-Dimensional Tissue Culture Based on Magnetic Cell Levitation,” Nature Nanotechnology 5, no. 4 (April 2010): 291–96, doi:10.1038/nnano.2010.23. Jennifer R. Molina et al., “Invasive Glioblastoma Cells Acquire Stemness and Increased Akt Activation,” Neoplasia 12, no. 6 (June 2010): 453–IN5, doi:10.1593/neo.10126. Neenu Singh et al., “Potential Toxicity of Superparamagnetic Iron Oxide Nanoparticles (SPION),” Nano Reviews 1, no. 0 (September 21, 2010), doi:10.3402/nano.v1i0.5358. Ali S. Arbab et al., “Characterization of Biophysical and Metabolic Properties of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles and Transfection Agent for Cellular MR Imaging,” Radiology 229, no. 3 (December 2003): 838–46, doi:10.1148/radiol.2293021215. Farideh Namvar et al., “Cytotoxic Effect of Magnetic Iron Oxide Nanoparticles Synthesized via Seaweed Aqueous Extract,” International Journal of Nanomedicine, May 2014, 2479, doi:10.2147/IJN.S59661. Marcela Gonzales et al., “Cytotoxicity of Iron Oxide Nanoparticles Made from the Thermal Decomposition of Organometallics and Aqueous Phase Transfer with Pluronic F127,” Contrast Media & Molecular Imaging 5, no. 5 (September 2010): 286–93, doi:10.1002/cmmi.391. Yu Pan, Matthias Bartneck, and Willi Jahnen-Dechent, “Cytotoxicity of Gold Nanoparticles,” in Methods in Enzymology, vol. 509 (Elsevier, 2012), 227. Stefaan J.H. Soenen and Marcel De Cuyper, “How to Assess Cytotoxicity of (Iron Oxide-Based) Nanoparticles. A Technical Note Using Cationic Magnetoliposomes,” Contrast Media & Molecular Imaging 6, no. 3 (May 2011): 153–64, doi:10.1002/cmmi.415. Stefaan J.H. Soenen et al., “Cytotoxic Effects of Iron Oxide Nanoparticles and Implications for Safety in Cell Labelling,” Biomaterials 32, no. 1 (January 2011): 195–205, doi:10.1016/j.biomaterials.2010.08.075.

Daniele Foresti et al., “Investigation of a Line-Focused Acoustic Levitation for Contactless Transport of Particles,” Journal of Applied Physics 109, no. 9 (2011): 93503. Ochiai, Takayuki, and Rekimoto, “Three-Dimensional Mid-Air Acoustic Manipulation by Ultrasonic Phased Arrays.”

• D. Foresti, M. Nabavi, and D. Poulikakos, “On the Acoustic Levitation Stability Behaviour of Spherical and Ellipsoidal Particles,” Journal of Fluid Mechanics 709 (October 2012): 581–92,

doi:10.1017/jfm.2012.350. Bazou et al., “Long-Term Viability and Proliferation of Alginate-Encapsulated 3-D HepG2 Aggregates Formed in an Ultrasound Trap.” Baresch, Thomas, and Marchiano, “Observation of a Single-Beam Gradient Force Acoustical Trap for Elastic Particles.” Yoichi Ochiai, Takayuki Hoshi, and Jun Rekimoto, “Pixie Dust: Graphics Generated by Levitated and Animated Objects in Computational Acoustic-Potential Field,” ACM Transactions on Graphics 33, no. 4 (July 27, 2014): 1–13, doi:10.1145/2601097.2601118. Baresch, Thomas, and Marchiano, “Observation of a Single-Beam Gradient Force Acoustical Trap for Elastic Particles.” Jean-Louis Thomas and Antoine Riaud, “How to Generate a Swirling Surface Acoustic Wave?,” Fait d’Actu, December 2015, INSP-Institut des Nanosciences de Paris edition. Baresch, “Pince Acoustique.”

• A. Schuster et al., “Cell Specific Ultrasound Effects Are Dose and Frequency Dependent,” Annals of Anatomy - Anatomischer Anzeiger 195, no. 1 (January 2013): 57–67,

doi:10.1016/j.aanat.2012.03.008.

• R. Buckminster Fuller, Synergetics (Pacific Tape Library, 1975)