Phenotyping & Breeding MARS Dissemination event Madrid, 29 - - PowerPoint PPT Presentation

MARS Dissemination event Madrid, 29 October 2015

Phenotyping & Breeding

MARS results:

Genotyping Phenotyping

×

Resistant

Cultivars resistant to PPV

Breeding for sharka resistance

Resistant or Susceptible

Segregating Populations Market demands

‐ Local cultivars ‐ Adapted

Resistant genotypes

‐ Selection ‐ Characterization ‐ PPV Resist. validation

Breeding for sharka resistance

Genotyping Set 1: PGS1‐21/ZP002/PGS1‐24

43,000 samples

Set 2: PGS1‐10/AMMPPG016 AMMPPG021/SSRLG1‐11m52

23,000 samples

Resistant or recombinant

Greenhouses Quarantine shelter Commercial and experimental orchards

Phenotyping the PPV resistance

GF305 rootstock Inoculum Cultivar

Greenhouse Field and Quarantine Shelter Evaluation methods

PPV Resistance confirmed by genotype and phenotype Floral biology: ‐ Chill requirements, self‐compatibility, etc. Fructification: ‐ Fruit set, productivity, ripening date, etc. Fruit quality: ‐ Weight, color, firmness, sugar content, acidity, etc. Other agronomical traits: ‐ Vigour, growth habit, level of thining…

Phenotypic characterization of population

Results Partners: 01 INRA & 11 CEP innovation (France)

4 New apricot cultivars

11,192 seedlings 6,850 screened by markers 24 crosses 4000 hybrids in orchard 300 pre‐selections 20 selections

MAS for PPVRes and self‐fertility

Results Partners: 02 UMIL & 03 UNIBO & 12 CRPV (Italy) 60% Susceptible 35% Heterozygous resistant 5% Homozygous resistant 47 selections

317 Accessions

7 Markers

PPV analysis and Agronomical studies

Dice’s coefficient 0.77 0.82 0.88 0.94 1.00 BO81604311 Harcot Goldrich SEO Harlayne Lito Perlecot Reale

28 SNPs

Genetic diversity in the Sharka resistance region

‐ Screened 6000 seedlings ‐ 2 selections will be patent in 2016

244 seedlings Genotyped 7 Markers Phenotyped (80 seedlings) 55 seedling (Resistance alleles) “Pyramiding” 2 crosses: ‐ 50 seedlings

Results Partner 04: USAMV (Romania)

SEO NJA 83 Traian SharCo

11 crosses 348 seedlings 105 seedlings (Resistance alleles)

Results Partner 05: FGI Plodiv (Bulgaria)

Resistant parents Harlayne NJ A2 Lito Goldrich

84 new hybrids under genotyping

Several activities of dissemination have been carried out in 2015

84 hybrids tranfer to Juglans Regia

Results Partner 06: Mustafa Kemal University (Turkey)

570 progenies with resistant alleles 311 progenies with recombinant alleles 1,755 Progenies 3 Markers (1st Set)

Molecular screening studies are still in progress 14 Local cultivars × SEO, Stella, Harlayne, Robada

Results Partner 06: Mendel University (Czech Republic)

200 seedlings Phenotyped with D and Rec

Harlayne × Betinka Tolerant Resistant Sensitive Uncertain 63 seedlings 117 seedlings 2 seedlings 15 seedlings Hybrids H998

20 selections under field evaluation

Results Partner 07: HAO DEMETER former NAGREF (Greece)

6 new cultivars: ‐ Chariessa ‐ Danais ‐ Neraida ‐ Tyrvi ‐ Laias ‐ Niriis 10 North American Cultivars Bebecou & Proimo Tyrinthou Self‐fertile, Resistant to PPV and Very good agronomical characteristics

“Pyramiding” Several crosses 270 seedlings Screened about 400 seedlings

Results Partner 08: CEBAS‐CSIC (Spain) 360 seedlings PPV‐D + 3 Markers

• O. Red & Goldrich

× Búlida P., Currot, Palstein, T. Bordaneil 180 completely evaluated: ‐ Resistant 101 (16 Suscep. alleles) ‐ Susceptible 79 (15 Resist. alleles) “Pyramiding” crosses 6 crosses: ‐ 350 seedlings ‐ 288 phenotyping ‐ 140 genotyping

5 New apricot selections

2nd set of Markers

Results Partner 14: Juglans Regia (Bulgaria)

84 hybrids 60 hybrids

Under agronomical evaluation

FGI Plodiv

Several activities of dissemination have been carried out in 2015 with FGI Plodiv

Results Partner 15: EFE viveros del sureste (Spain)

Dissemination (WP3) ‐ Two open day (training workshop)s attended by about 200 people (30th of April 2014, and 22th May 2015). ‐ Promotion of PPV resistant breeding and pre‐breeding material for plantation (Leaflets, advertisements, etc.) Agronomical evaluation (WP2) ‐ Six apricot cultivars resistant to PPV (Rojo Pasión, Mirlo Blanco, Mirlo Anaranjado, Mirlo Rojo, Murciana and Valorange). ‐ Acquisition of the new five apricot selections resistant to PPV ‐ Growing resistant cultivars PPV ‐ Monitoring and maintenance of new apricot varieties resistant

Results Partner 16: Fytoria Karayiannis (Greece)

Several activities of dissemination Agronomical evaluation (WP2)

POSTER SESSION