Phenotyping & Breeding MARS Dissemination event Madrid, 29 - PowerPoint PPT Presentation

MARS results: Phenotyping & Breeding MARS Dissemination event Madrid, 29 October 2015

Breeding for sharka resistance × Resistant Resistant or Susceptible ‐ Local cultivars ‐ Adapted Segregating Populations Genotyping Phenotyping Market Cultivars resistant to PPV demands

Breeding for sharka resistance Genotyping 43,000 samples Set 1: PGS1‐21/ZP002/PGS1‐24 Resistant or recombinant 23,000 samples Set 2: PGS1‐10/AMMPPG016 AMMPPG021/SSRLG1‐11m52 ‐ Selection Resistant genotypes ‐ Characterization ‐ PPV Resist. validation

Phenotyping the PPV resistance Quarantine shelter Greenhouses Commercial and experimental orchards

Evaluation methods GF305 Cultivar rootstock Inoculum Greenhouse Field and Quarantine Shelter

Phenotypic characterization of population PPV Resistance confirmed by genotype and phenotype Floral biology: ‐ Chill requirements, self‐compatibility, etc. Fructification: ‐ Fruit set, productivity, ripening date, etc. Fruit quality: ‐ Weight, color, firmness, sugar content, acidity, etc. Other agronomical traits: ‐ Vigour, growth habit, level of thining…

Results Partners: 01 INRA & 11 CEP innovation (France) 24 crosses 6,850 screened by markers 11,192 seedlings MAS for PPVRes and self‐fertility 4000 hybrids in orchard 300 pre‐selections 20 selections 4 New apricot cultivars

Results Partners: 02 UMIL & 03 UNIBO & 12 CRPV (Italy) 7 Markers 28 SNPs 317 Accessions Genetic diversity in the Sharka resistance region BO81604311 Harcot 60% Susceptible Goldrich SEO 35% Heterozygous resistant Harlayne 5% Homozygous resistant Lito Perlecot Reale 0.77 0.82 0.88 0.94 1.00 Dice’s coefficient PPV analysis and Agronomical studies 47 selections ‐ Screened 6000 seedlings ‐ 2 selections will be patent in 2016

Results Partner 04: USAMV (Romania) SharCo 244 seedlings Genotyped 7 Markers Phenotyped (80 seedlings) 55 seedling (Resistance alleles) “Pyramiding” 2 crosses: ‐ 50 seedlings SEO NJA 83 Traian

Results Partner 05: FGI Plodiv (Bulgaria) Resistant parents Harlayne NJ A2 11 crosses 348 seedlings Lito Goldrich 105 seedlings (Resistance alleles) 84 new hybrids under genotyping 84 hybrids tranfer to Juglans Regia Several activities of dissemination have been carried out in 2015

Results Partner 06: Mustafa Kemal University (Turkey) 14 Local cultivars × 1,755 Progenies 3 Markers (1st Set) SEO, Stella, Harlayne, Robada 570 progenies with resistant alleles 311 progenies with recombinant alleles Molecular screening studies are still in progress

Results Partner 06: Mendel University (Czech Republic) Harlayne × Betinka 200 seedlings Phenotyped with D and Rec Hybrids H998 Tolerant Resistant Sensitive Uncertain 63 seedlings 117 seedlings 2 seedlings 15 seedlings 20 selections under field evaluation

Results Partner 07: HAO DEMETER former NAGREF (Greece) 10 North American Bebecou & Cultivars Proimo Tyrinthou Screened about 400 seedlings 6 new cultivars: “Pyramiding” ‐ Chariessa ‐ Danais ‐ Neraida ‐ Tyrvi Several crosses ‐ Laias ‐ Niriis 270 seedlings Self‐fertile, Resistant to PPV and Very good agronomical characteristics

Results Partner 08: CEBAS‐CSIC (Spain) O. Red & Goldrich × 360 seedlings PPV‐D + 3 Markers Búlida P., Currot, Palstein, T. Bordaneil 2 nd set of Markers 180 completely evaluated: ‐ Resistant 101 (16 Suscep. alleles) ‐ Susceptible 79 (15 Resist. alleles) “Pyramiding” crosses 6 crosses: ‐ 350 seedlings 5 New apricot selections ‐ 288 phenotyping ‐ 140 genotyping

Results Partner 14: Juglans Regia (Bulgaria) FGI Plodiv 84 hybrids 60 hybrids Under agronomical evaluation Several activities of dissemination have been carried out in 2015 with FGI Plodiv

Results Partner 15: EFE viveros del sureste (Spain) Agronomical evaluation (WP2) ‐ Six apricot cultivars resistant to PPV (Rojo Pasión, Mirlo Blanco, Mirlo Anaranjado, Mirlo Rojo, Murciana and Valorange). ‐ Acquisition of the new five apricot selections resistant to PPV ‐ Growing resistant cultivars PPV ‐ Monitoring and maintenance of new apricot varieties resistant Dissemination (WP3) ‐ Two open day (training workshop)s attended by about 200 people (30th of April 2014, and 22th May 2015). ‐ Promotion of PPV resistant breeding and pre‐breeding material for plantation (Leaflets, advertisements, etc.)

Results Partner 16: Fytoria Karayiannis (Greece) Agronomical evaluation (WP2) Several activities of dissemination POSTER SESSION