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Why I love my job: The enabling influence of breeding We are here - - PowerPoint PPT Presentation
Why I love my job: The enabling influence of breeding We are here - - PowerPoint PPT Presentation
Why I love my job: The enabling influence of breeding We are here Stan Allen ABC, VIMS Biomass pyramid Breeding Genetic services Multiplier population Brood stock services Hatchery production Commercial grow out Biomass pyramid Breeding
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Biomass pyramid
Hatchery production Commercial grow out Multiplier population Breeding Genetic services Brood stock services Economic multiplier
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Breeding Genetic services: MASS SELECTION
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MASS SELECTION: Lines to Superlines in 2008
LINE ORIGIN GENES DDBY DEBY D FBST DEBY D,X ADMO DEBY D,W ADMOW DEBY D,W ADXB DEBY,XB D,X ADXBW DEBY,XB D,X BXB XB X BXBW XB X CAMX XB,CAM X,L ZOBOY OBOY L ZOBOYX OBOY L ZLGT LGT L ZLGTK LGT L ZLGTW LGT L YDBLA DEBY,LGT D,L
LA XB DBY
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MASS SELECTION: DR + growth rate
X X X
York Kinsale Lynnhaven Low High High Lola hANA DBY LA XB
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Breeding Genetic services: FAMILY SELECTION
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FAMILY SELECTION: Since 2010 ♂ ♀ x
brothers and sisters
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FAMILY SELECTION: Test sights
X X X X
Lynnhaven York high Lewisetta Cambridge low
~ 150 families
- ~150,000 oysters
- ~750 reps deployed
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FAMILY SELECTION: Correlation low (HPL) vs. high (VIMS) salinity TOTAL WEIGHT HIGH SALINITY TOTAL WEIGHT LOW SALINITY
Genetic correlation rg = 0.7
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- 1.5
- 1
- 0.5
0.5 1 1.5
- 1.5
- 1
- 0.5
0.5 1 Survival Low salinity (GSD) Survival High salinity (GSD)
SURVIVAL: high - low salinity sites
SURVIVAL HIGH SALINITY SURVIVAL LOW SALINITY FAMILY SELECTION: Correlation low (HPL) vs. high (VIMS) salinity
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Breeding Genetic services: TETRAPLOID SELECTION
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2n Breeding legacy
20+ years 4 years
4n
disease resistance
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4n breeding objectives Survival Growth rate
TETRAPLOID SELECTION: The fathers of triploids
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FAMILY SELECTION: Test sights
X X
York Cambridge
~ 50 families
- ~50,000 oysters
- ~250 reps deployed
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Multiplier population Brood stock services: MASS SELECTION
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MULTIPLIER POPULATION: Mass Selection
discards selected distributed to hatcheries
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Multiplier population Brood stock services: FAMILY SELECTION TETRAPLOID SELECTION
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Top families Diploid: Lily, Hnry Tetraploid: FYR MULTIPLIER POPULATION: family breeding
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Mass selection 2n 4n Family selection 2n 4n DEBY XB Lola hANA HNRY LILY 4GEN 4GNL 4NGN (2019) 4FYR (2019?) Brood stock available from ABC
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Tetraploid Diploid 5 10 15 20 25 30 07 08 09 10 11 12 13 15 14 18 16 17 Thousands Year Brood stock distributed by ABC
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LINE S SELECTION SITE ORIGIN ATTRIBUTES NOTES XB
Founder population strarted by Allen at HSRL using Haskin lines in 1990s, then selection in VA, new material added in 2008 Mass selection for MSX, continuous exposure to ambient Dermo, seems to favor higher salinity
DEBY-Lynn
Wild Del Bay oysters selected at VIMS in York by Burreson group in 80s, new material added in 2008 Mass selection for MSX, continuous exposure to ambient Dermo, general all purpose across salinity ranges
hANA
Founders from three wild populations in Louisiana ca. 2000-2004, new material added in 2008 Mass selection for MSX, continuous exposure to ambient Dermo, grows (too?) fast, mortality in some places Discontinued starting 2018
HNRY
Founders from wild and selected populations ca. 2005, family selection program, top families form terminal brood stock population Family selection for high survival in high salinity, continuous exposure to ambient Dermo, co- selected for shape, growth rate, meat yield
Released 2017
DEBY-Lew ~15 York, then Coan R.
Wild Del Bay oysters selected at VIMS in York by Burreson group in 80s, new material added in 2008 Mass selection for MSX before low salnity selection began in 2008, low ambient Dermo exposure, not in high demand Discontinued starting 2018
Lola
Founders from three wild populations in Louisiana ca. 2000-2004, new material added in 2008 Mass selection for MSX up to 2008, then continuously held at low salinity, low exposure to Dermo, widely popular at various salinity
LILY
Founders from wild and selected populations ca. 2005, family selection program, top families form terminal brood stock population Family selection for high survival in low salinity, low exposure to ambient Dermo, co-selected for shape, growth ratem, meat yield
Released 2017
18-25 York R. and Lynnhaven Coan R. Va Choptank R. Md 8-15 DIPLOID
All brood stock (2n)
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LINE S SELECTION SITE ORIGIN ATTRIBUTES NOTES 4GEN --> 4NGN
Original tetraploid line derived from spawns of disease resistant oysters ca. early 2000s and propagated for ~10 generations
Highly domesticated, disease resistant,some inbreeding, variable growth rates
4GNL
4VBOY tetraploids (with Louisiana germ plasm) are hybridized with 4GENs to make F1 brood stock More robust, more uniform, must be made anew each generation requiring maintenance of 4VBOY line Released 2017
8-12 Choptank R. Md
Breeding for usual traits, teraploid stability at high salinity
18-22 York R. Va
Breeding for usual traits, teraploid stability at low salinity Founder populations from various old and new tetraploid lines contributed to tetraploid family breeding program Likely line release 2019
18-25 York R. and Lynnhaven TETRAPLOID 4FML