our goal to encourage growth and expansion of the igem
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Our goal: to encourage growth and expansion of the iGEM competition. - PDF document

May 06, 2023 •289 likes •378 views

06/11/2010 Our goal: to encourage growth and expansion of the iGEM competition. What are the limits of the most common One cell = one compartment. iGEM chassis ( E. coli )? 1 06/11/2010 Plasmid capacity. Movement speed and range. 2

  1. 06/11/2010 Our goal: to encourage growth and expansion of the iGEM competition. What are the limits of the most common One cell = one compartment. iGEM chassis ( E. coli )? 1

  2. 06/11/2010 Plasmid capacity. Movement speed and range. 2

  3. 06/11/2010 Bacteria ( E. coli ) C. elegans Agar plates Easy maintenance Short life cycle Antibiotic selection Glycerol stocking Familiar? SID-2 (dsRNA transporter) A property of many promoters 3

  4. 06/11/2010 promoter brick coding brick terminator • Superior locomotion • Extensive sensory capabilities • Take advantage of E. coli parts 4

  5. 06/11/2010 Inter-tissue communication (e.g. nervous system to muscular system) Ion channels: • Channelrhodopsin-2 (ChR2) • Halorhodopsin (NpHR) Tissue-Specific Constitutive pMec-7 mechanosensory neurons pGpd-2 > pRab-7 > pSip-1 pOdr-10 AWA olfactory neuron pStr-220 AWB olfactory neuron pStr-1 AWB chemosensory neuron Inducible pOdr-1 AWC chemosensory neuron pOsm-10 ASH osmosensory neuron pHsp-3 (enhanced by chemical stress) pFlp-1 AVA sensory neuron pSra-10 AVB sensory neuron 5

  6. 06/11/2010 eGFP Green fluorescence marker eCFP Cyan fluorescence marker eYFP Yellow fluorescence marker mCherry Red fluorescence marker STOP ChR2 Channelrhodopsin-2 unc-54 3' UTR contains poly-A signal NpHR Halorhodopsin BBa_K309000 pMec-7 Promoter BBa_K309012 unc- T erminator BBa_K309001 pSra-10 Promoter BBa_K309013 pOsm-10 Promoter BBa_K309002 pGpd-2 Promoter BBa_K309014 pOdr-1 Promoter BBa_K309003 pStr-1 Promoter BBa_K309015 NpHR::eCFP T agged effector BBa_K309004 ChR2::eYFP T agged effector BBa_K309016 eGFP Reporter BBa_K309005 mCherry Reporter BBa_K309017 pStr-220 Promoter BBa_K309006 pSip-1 Promoter BBa_K309018 eYFP Reporter BBa_K309007 ChR2 Effector BBa_K309019 NpHR Effector BBa_K309009 pOdr-10 Promoter BBa_K309020 eCFP Reporter BBa_K309010 pFlp-1 Promoter BBa_K309021 pStr-220::eCFP Full construct BBa_K309011 pHsp-3 Promoter BBa_K309022 pOdr-10::eCFP Full construct 6

  7. 06/11/2010 ALML PLML AVM BBa_K309023 pGpd-2::eCFP Full construct BBa_K309024 pSip-1::eCFP Full construct PVM ALMR PLMR BBa_K309025 pFlp-1::eCFP Full construct BBa_K309026 ChR2::eYFP T agged effector + terminator BBa_K309027 pHsp-3::eCFP Full construct Part:BBa_K309032 BBa_K309028 pOsm-10::eCFP Full construct pMec-7::eCFP::unc-54 3' UTR BBa_K309029 mCherry Reporter + terminator BBa_K309030 eYFP Reporter + terminator BBa_K309031 pOdr-1::eCFP Full construct BBa_K309032 pMec-7::eCFP Full construct PLMR Kozak sequence around start codon GCC ACC AUG G Optimizes translation 7

  8. 06/11/2010 • WormBase huge gene databank • WormBook thorough research review/textbook • WormAtlas where to start with worms • WormMethods protocols • GFPWorm where promoters function • Promoterome known minimal promoters • UTRome and UTRdb 3' UTRs • Feasibility? • Dr. Ian Chin-Sang, and all of our other advisors • • VWR, for discounts on reagents • Fundamental parts • Octane Biotech, for their generous • New standard contribution • WormGuide • -going • Go forth! support • And you, for watching. 8

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