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Organic Compounds in Water and Wastewater Cyanotoxins Occurrence - PowerPoint PPT Presentation

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Print version CEE 697z Organic Compounds in Water and Wastewater Cyanotoxins Occurrence & Chemical Analysis Lecture #28 CEE 697z - Lecture #28 Microcystin Occurrence in US CEE 697z - Lecture #28 Anatoxin CEE 697z - Lecture #28

  1. Print version CEE 697z Organic Compounds in Water and Wastewater Cyanotoxins Occurrence & Chemical Analysis Lecture #28 CEE 697z - Lecture #28

  2. Microcystin Occurrence in US CEE 697z - Lecture #28

  3. Anatoxin CEE 697z - Lecture #28

  4. Cylindrospermopsin CEE 697z - Lecture #28

  5. From: “ Analytical Methods for Cyanotoxin Detection and Analysis Impacts on Data Interpretation ”, Loftin et al., 2010, USG CEE 697z - Lecture #28

  6. Analysis Fig. 5 Overview of sample preparation and analytical methods for the detection of cyanotoxins. Merel, S., Walker, D., Chicana, R., Snyder, S., Baures, E. and Thomas, O. (2013) State of knowledge and concerns on cyanobacterial blooms and cyanotoxins. Environment International 59, 303-327. CEE 697z - Lecture #28

  7. Analytical Specificity From: “ Analytical Methods for Cyanotoxin Detection and Impacts on Data Interpretation ”, Loftin et al., 2010, USGS CEE 697z - Lecture #28

  8. Selectivity & Sensitivity T oxic Cyanobacteria in Water: A guide to their public health consequences, CEE 697z - Lecture #28 monitoring and management, Edited by Ingrid Chorus and Jamie Bartram , 1999 WHO

  9. Example: Analysis of BMAA I Sample preparation scheme for determining total BMAA in cyanobacteria (D 3 -BMAA internal standard was added only to samples for quantification) CEE 697z - Lecture #28

  10. Analysis of BMAA II CEE 697z - Lecture #28 ESI-MS/MS product ion spectra of ( a ) BMAA and ( b ) D 3 -BMAA

  11. WRF Study on Methods  2007, “Determination and Significance of Emerging Algal Toxins  Nicholson et al.  Analytes  Anatoxin-a  Saxitoxin (STX) & derivatives (PSTs)  Cylindrospermopsin (CYN) CEE 697z - Lecture #28

  12. Preferred Method  LC/MS/MS selected  HILIC for highly polar PSTs  Hydrophilic interaction liquid chromatography  Prior SPE  procedure CEE 697z - Lecture #28

  13. Selection of SPE phase Carbograph is non-porous graphitized carbon black. Its surface contains oxygen complexes forming positively charged chemical heterogeneities on surface offering CEE 697z - Lecture #28 rapid both primary reversed-phase and secondary anion exchange interaction

  14. CEE 697z - Lecture #28

  15. CEE 697z - Lecture #28

  16. CEE 697z - Lecture #28

  17. CEE 697z - Lecture #28

  18.  To next lecture CEE 697z - Lecture #28

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