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CEE 697z
Organic Compounds in Water and Wastewater
Cyanotoxins Occurrence & Chemical Analysis
CEE 697z - Lecture #28
Microcystin Occurrence in US
CEE 697z - Lecture #28
Organic Compounds in Water and Wastewater Cyanotoxins Occurrence - - PowerPoint PPT Presentation
Organic Compounds in Water and Wastewater Cyanotoxins Occurrence - - PowerPoint PPT Presentation
Print version CEE 697z Organic Compounds in Water and Wastewater Cyanotoxins Occurrence & Chemical Analysis Lecture #28 CEE 697z - Lecture #28 Microcystin Occurrence in US CEE 697z - Lecture #28 Anatoxin CEE 697z - Lecture #28
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Anatoxin
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Cylindrospermopsin
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Analysis
From: “Analytical Methods for Cyanotoxin Detection and Impacts on Data Interpretation”, Loftin et al., 2010, USG
CEE 697z - Lecture #28
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- Fig. 5 Overview of sample
preparation and analytical methods for the detection of cyanotoxins.
Merel, S., Walker, D., Chicana, R., Snyder, S., Baures, E. and Thomas, O. (2013) State of knowledge and concerns on cyanobacterial blooms and
- cyanotoxins. Environment
International 59, 303-327.
Analysis
CEE 697z - Lecture #28
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Analytical Specificity
From: “Analytical Methods for Cyanotoxin Detection and Impacts on Data Interpretation”, Loftin et al., 2010, USGS
CEE 697z - Lecture #28
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T
- xic Cyanobacteria in Water: A guide to their public health consequences,
monitoring and management, Edited by Ingrid Chorus and Jamie Bartram , 1999 WHO
Selectivity & Sensitivity
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Sample preparation scheme for determining total BMAA in cyanobacteria (D3-BMAA internal standard was added only to samples for quantification)
Example: Analysis of BMAA I
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ESI-MS/MS product ion spectra of (a) BMAA and (b) D3-BMAA
Analysis of BMAA II
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WRF Study on Methods
2007, “Determination and Significance of Emerging Algal
Toxins
Nicholson et al.
Analytes
Anatoxin-a Saxitoxin (STX) & derivatives (PSTs) Cylindrospermopsin (CYN)
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Preferred Method
LC/MS/MS selected HILIC for highly polar PSTs
Hydrophilic interaction liquid chromatography
Prior SPE
procedure
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Selection of SPE phase
Carbograph is non-porous graphitized carbon black. Its surface contains oxygen complexes forming positively charged chemical heterogeneities on surface offering rapid both primary reversed-phase and secondary anion exchange interaction
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CEE 697z - Lecture #28