Harvesting solar power with anthocyanins iGEM Team TU Darmstadt 2014 - - PowerPoint PPT Presentation

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Harvesting solar power with anthocyanins iGEM Team TU Darmstadt 2014 - - PowerPoint PPT Presentation

Sep 29, 2023 360 likes •583 views

Harvesting solar power with anthocyanins iGEM Team TU Darmstadt 2014 Electricity & Poverty 8 UN Millennium Development Goals to fight hunger & poverty Access to electricity leads to Additional hours spent learning by children

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SLIDE 1

Harvesting solar power with anthocyanins

iGEM Team TU Darmstadt 2014

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SLIDE 2

Electricity & Poverty

2

Access to electricity leads to

  • Additional hours spent learning by children
  • Additional productivity
  • Improved health
  • Increased household net income

8 UN Millennium Development Goals to fight hunger & poverty

Africa at night in 2014

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SLIDE 3

3

DSCs (or Grätzel cells) are

  • Cheaper
  • Don’t need rare metals
  • More environment-friendly

than classical solar cells DSCs can

  • Utilize diffuse light
  • Light from every angle
  • Be used in warm regions

Dye-sensitized solar cells (DSC)

  • vs. classical solar cells
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SLIDE 4

Dye-sensitized solar cells

4

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SLIDE 5

Anthocyanins

  • Flavonol derived plant pigments
  • Found in higher plants
  • Can be used effectively in Grätzel cells
  • Heterologous production in E. coli feasible

5

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SLIDE 6

Application scenario

6

Local partner Villagers Government / NGOs Local industry DSC producer Local microcredit bank

Purchasing machines Developing concepts Providing capital Cooperation Purchasing DSCs Lending money Training Selling DSCs

Conclusions:

  • 1. Project will be applied in hot rural areas
  • 2. Adjusting our pathway
  • 3. Examining “soft” aspects by a techno-moral vignette
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SLIDE 7

Meeting the experts

7

Visiting Prof. Dr. Grätzel and Dr. Toby Meyer of Solaronix

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SLIDE 8
  • Broad variety of anthocyanins in

plants

  • Production yield in plants not

predictable

  • Easy extraction from E. coli
  • No unwanted side products
  • Higher yield in shorter time with

less space consumption

Why in E. coli?

8 pelargonidin

Katsumuto et al., 2007

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SLIDE 9

The pathway

9

Central branching point Splitting of

  • ur pathway
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SLIDE 10

iGEM Uppsala 2013 BBa_K1497001 BBa_K1497000 BBa_K1033000 BBa_K1033001 chi chi 4-cl cl chs chs tal R R R R

From tyrosine to naringenin

naringenin producing operon (BBa_K1497007) 10

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SLIDE 11

Naringenin biosensor (1)

11 naringenin FdeR homodimer naringenin bound to FdeR homodimer

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SLIDE 12

A: Naringenin biosensor with CFP as reporter (BBa_K1497022) B: Naringenin biosensor with mKate as reporter (BBa_K1497021) C: Naringenin biosensor without a reporter (BBa_K1497019) D: Naringenin biosensor with GFP as reporter (BBa_K1497020)

Naringenin biosensor (2)

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GFP and mKate biosensor combined have a broader range!

GFP mKate

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SLIDE 13

Quantification of naringenin production

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Quick comparison of different constructs! T7 operon: ≈ 3 µM

  • Const. operon: ≈ 1 µM

after 16 h of incubation

500 1000 1500 2000 2500 3000 3500 4000

Relative Fluorescence Units (RFU)

  • Neg. control

T7 naringenin operon (BBa_K1497017)

  • Const. naringenin operon

(BBa_K1497016)

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SLIDE 14

BBa_K1497011 BBa_K1497010 BBa_K1497009

From naringenin to pelargonidin

14 dfr dfr f3h f3h ans ans R R R BB BBa_K1497023

No glycosylation -> extraction with organic solvents possible!

pelargonidin

pelargonidin extracted with dichloromethane

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SLIDE 15

DFR ANS

Metabolic channeling

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protein scaffold by iGEM Team SJTU-BioX-Shanghai 2012

SH3 GBD PDZ

naringenin pelargonidin

F3H

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SLIDE 16

Modularization

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Construction of scaffold variations by BglII Brick cloning strategy

See SCUT 2014 for S. cerevisiae

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SLIDE 17
  • Elastic Network Models
  • Linear Response Theory
  • All Atom Molecular Dynamics

Elastic Network Model

Dry lab to wet lab

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Modeling lead to shortened anthocyanidin synthase (eANS, BBa_K1497002)

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SLIDE 18
  • Modularizable Grätzel cell holder
  • Printed with bio-degradable PLA
  • First test: ≈ 0.6 mA and 150 mV !
  • See the models at our poster

Mission accomplished!

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SLIDE 19

Achievements

  • Characterizing 23 of 33 BioBrick parts

sent to the registry

  • Construction of a functional

pelargonidin pathway in E. coli

  • Engineering and improving of ANS by in

silico protein multi-scale modeling

  • Introducing three functional naringenin

biosensors

  • Introducing an improved protein scaffold
  • Describing a novel Policy & Practices

approach

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SH3 GBD PDZ

pelargonidin extracted with dichloromethane

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SLIDE 20

More achievements!

20

Visit our Wiki for:

  • Our Safety approach
  • More modeling data
  • More scaffold improvements
  • Open Hardware models (3D-printer

STL files)

  • Open Software packages for R
  • Our full application scenario &

techno-moral vignette

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SLIDE 21

Acknowledgements

21 The Team

Rico Ballmann Sebastian Barthel Malte Blumenroth Thomas Dohmen Max Dombrowsky Kai Fenzl Tobias Gabriel Sascha Hein Niklas Hummel Carmen Klein Kai Kucharzewski Benjamin Mayer Christian Mende Laurin Monnheimer Sebastian Palluk Sven Rumpf Fabian Rohden Daniel Sachs Renè Sahm Christian Sator Andreas Schmidt Christian Sürder Michael Sürder Bastian Wagner Alex Wyllie

Advisors

  • Prof. Dr. Heribert Warzecha

Sven Jager

Thank you!

  • Prof. Dr. Katja Schmitz
  • Prof. Dr. Kay Hamacher
  • Prof. Dr. Heinz Koeppl
  • Prof. Dr. Jörg Simon
  • Prof. Dr. Adam Bertl
  • Prof. Dr. Gerhard Thiel

PD Dr. Tobias Meckel Sabine Fräbel Henning Pennekamp Jascha Volk

  • Dr. Melanie Kern
  • Dr. Stefan Martens

Alexander Schlauer Barbara Wolf Anne Einhäupl

  • Prof. Dr. Michael Grätzel
  • Dr. Toby Meyer
  • Prof. Dr. Alfred Nordmann

Wieke Betten Charlotte Kaspar