Wild Blueberries & Health Research Update WBPANS November 18, - PowerPoint PPT Presentation

Wild Blueberries & Health Research Update WBPANS November 18, 2016

Fruits, Vegetables and Human Health Research Publications From To Publications per Year Plant Foods 1980 1990 0.4 & Human Health 1991 2000 12 2001 2006 68 Berries and 2007 2012 176 especially blueberries 2013 2016 > 558 lead a major trend in food & health research

Blueberry Health and Nutrition Research

Discussion Topics: A Consumer Focused Plan • Topline Research Learnings • Key Change in Thinking • Early Blueberry Health Research Marketing Objectives & Strategies • Target Audience Focus • Topline Messaging Strategy Blueberry Antioxidants Fight Oxidative Stress • Creative Expression of New Campaign • Building The Fully Integrated Plan • Influencer Efforts (PR & Social) • Key Trade Initiatives • Online Focus • 2014 Budget Overview, 2013 Remaining • 2014 Activity Summary Calendar

Free Radical Theory of Aging OXIDATIVE STRESS, ANTIOXIDANT PROTECTION HEALTH & DISEASE OXIDATIVE STRESS Oxidative damage to DNA, lipids, • protein molecules in the body Damage to biomolecules leads to • stress, disease, aging • Important Balance in the Body: • Oxidative Stress vs Antioxidant Protection • Blueberries have a high antioxidant capacity

Wild Blueberries & Antioxidant Capacity

Evidence for Health Benefits How is it gathered? Test tubes Populations Animals in vitro Epidemiological Human trials e.g. ORAC in vivo Clinical Evidence from Strong Evidence Strong Evidence Complementary & a living system mechanistic evidence

BERRIES Blueberries Anthocyanins Anthocyanins are Flavonoids HUMAN HEALTH

Where do we get our dietary anthocyanins? • Population (epidemiological) studies score anthocyanin intake based only on strawberry and blueberry intake. • The world’s strawberry production is 12-times greater than its blueberry production. • Blueberries contain > 20-times more anthocyanin than strawberries.

Discussion Topics: Evidence in this review… A Consumer Focused Plan Population studies • Topline Research Learnings or • Key Change in Thinking Blueberries • Marketing Objectives & Strategies Animal studies • Target Audience Focus or • Topline Messaging Strategy or and • Creative Expression of New Campaign Clinical studies • Building The Fully Integrated Plan Anthocyanins • Influencer Efforts (PR & Social) or • Key Trade Initiatives in vitro studies • Online Focus • 2014 Budget Overview, 2013 Remaining • 2014 Activity Summary Calendar

Brain Protection by Blueberries and Anthocyanins

1999 In cognitive and Motor Tests Blueberries protect rats from brain aging Journal of Gerontology 11 (3): 298 – 300. Blueberries reverse the effects of brain aging

Anthocyanins and Brain Function during Aging Population Studies

Selected animal studies reporting neuroprotection by blueberries SEARCH: blueberr* AND brain = 79 publications • Blueberry diets Increased success of neural grafts (Bickford et al. 2006) • Blueberries protect against oxidative stress-induced learning impairment (Duffy et al, 2008) • Blueberries increased success of neural grafts (McGuire et al. 2007) • Blueberry supplementation attenuates microglial activation in intraocular hippocampal grafts to aged hosts (Willis et al. 2010) • Blueberry diets reduced incidence of experimental autoimmune encephalomyelitis (Xin et al. 2012) • Blueberry diets protect the hippocampus after 56 Fe irradiation (Shukitt-Hale et al. 2013) Blueberries fight stresses: Traumatic, oxidative, inflammatory, disease

2010 Apr 14;58(7):3996-4000 * p<0.05 * * California Verbal Verbal Paired Associate Journal of Gerontology 11 (3): 298 – 300. Learning Test Learning Test

Clinical Study With Children European Journal of Nutrition, 2016

Discussion Topics: A Consumer Focused Plan • Topline Research Learnings Cardiovascular Protection • Key Change in Thinking • Marketing Objectives & Strategies Anti-Diabetes Protection • Target Audience Focus • Topline Messaging Strategy • Creative Expression of New Campaign • Building The Fully Integrated Plan Protection against Metabolic Syndrome • Influencer Efforts (PR & Social) • Key Trade Initiatives • Online Focus • 2014 Budget Overview, 2013 Remaining • 2014 Activity Summary Calendar

Markers of Metabolic Syndrome  High risk for Type 2 Diabetes  High risk for Cardiovascular Disease  Abdominal obesity  Elevated plasma lipids  Elevated blood pressure  Elevated insulin resistance  Elevated blood coagulation factors  Elevated plasma markers of inflammation Metabolic Syndrome Cardiovascular Disease

Blood vessels: More than just plumbing Vascular Endothelium, Vascular Smooth Muscle • Control of blood pressure • Control of blood glucose • Control of “other deliveries” • Control of blood clotting • Extensive ‘cross talk’ with blood and vessel wall Vascular dysfunction due to hypertension, atherosclerosis and inflammation

Anthocyanins Benefit Human Heart Health Population Studies

Human Clinical Research Target Population = Post-menopausal Women

First published online July 21, 2010; doi:10.3945/jn.110.124701 N=66; Age~50 yr; BMI~38; 8 week Journal of Gerontology 11 (3): 298 – 300.

21 young healthy men; wild BB powder drink Acute Effects of Blueberry Intake on Blood Flow in Humans

William T. Cefalu, M.D. Botanical Research Center Pennington Biomedical Research Center Louisiana State University System Journal of Gerontology 11 (3): 298 – 300.

Blueberries lower pig plasma cholesterol Effect of Dose on Blood Total Cholesterol 0.35 2.50 P=0.004 0.30 Total Cholesterol (mmol/L) 2.45 ACN (mg C3GE/g feed) 0.25 2.40 0.20 11.7% 2.35 0.15 2.30 BB Anthocyanins 0.10 2.25 0.05 0.00 2.20 0 1% 2% 4% Dose Blueberry Dose

P<0.05 * 1600 WBC/mm 2 1200 50 μ m 50 μ m * Control Diet Blueberry Diet 800 Control Blueberry Histological marker of Inflammation

Inflammatory mediators Low fat Diet High fat diet High fat diet + BB

Blueberries and Vision Human clinical study Animal study Population studies are underway

Discussion Topics: A Consumer Focused Plan • Topline Research Learnings • Key Change in Thinking • Marketing Objectives & Strategies Blueberry Anthocyanins are Absorbed and • Target Audience Focus • Topline Messaging Strategy Metabolized by the Body • Creative Expression of New Campaign • Building The Fully Integrated Plan • Influencer Efforts (PR & Social) • Key Trade Initiatives • Online Focus • 2014 Budget Overview, 2013 Remaining • 2014 Activity Summary Calendar

Anthocyanins in the Body Recent Findings Food Anthocyanins Anthocyanin products & metabolites in the body

• Our body retains anthocyanin metabolites • Anthocyanins are found in body tissues • Anthocyanin metabolites can enter the brain

Discussion Topics: Emerging Major ‘Take Home Messages ’ A Consumer Focused Plan • Topline Research Learnings • Key Change in Thinking • Anthocyanins affect the vascular system to • Marketing Objectives & Strategies • Target Audience Focus benefit health in various ways • Topline Messaging Strategy • Our body’s exposure to anthocyanins is complex • Creative Expression of New Campaign • Building The Fully Integrated Plan • Influencer Efforts (PR & Social) • Key Trade Initiatives • Online Focus • 2014 Budget Overview, 2013 Remaining • 2014 Activity Summary Calendar

Discussion Topics: Wild Blueberry Health Research Continues to Grow A Consumer Focused Plan • Topline Research Learnings Conferences in 2017 • Key Change in Thinking • Marketing Objectives & Strategies Berry Health Benefits Symposium • Target Audience Focus • Topline Messaging Strategy International Conference on Polyphenols and Health • Creative Expression of New Campaign 20 th Bar Harbour Wild Blueberry Summit • Building The Fully Integrated Plan • Influencer Efforts (PR & Social) • Key Trade Initiatives • Online Focus • 2014 Budget Overview, 2013 Remaining • 2014 Activity Summary Calendar

Wild Blueberry Health Research Continues to Grow clinicaltrials.gov • US/CANADA = 71% # Topic • Interventional = 97% Anti-ox & anti-inflammation 9 • BB juice or powder = 41% Bioavailability: pharmacology, metabolomics 5 • BB + 1 ingredient = 9% Cancer 3 • Multi-component Cardiovascular: endothelial function, blood 10 pressure, dyslipidemia, hyperglycemia supplements = 50% Gout 1 • Study size, n = 10-200 Metabolic syndrome: Appetite , 9 hypertension, obesity, diabetes, insulin sensitivity, • Study end date: hyperglycemia – ‘04 - ’06 = 4 Neuroscience: memory, aged-related memory, 12 cognitive decline, gene expression, headache, migraine – ‘07 - ’09 = 4 Vision 1 – ‘10 - ’13 = 17 Clinicaltrials.gov – 2014 & later = 9 SEARCH ‘Blueberries’ = 34 clinical trials

Bar Harbor Research Summit Since 1997

Thank You for Your Kind Attention Wilhelmina (Willy) Kalt wilhelmina.kalt@agr.gc.ca