Does DNA methylation facilitate phenotypic plasticity in marine - - PowerPoint PPT Presentation

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Does DNA methylation facilitate phenotypic plasticity in marine - - PowerPoint PPT Presentation

Aug 20, 2022 242 likes •872 views

Does DNA methylation facilitate phenotypic plasticity in marine invertebrates? Steven Roberts School of Aquatic and Fishery Sciences robertslab.info @sr320 Shameless plug onsnetwork.org/eimd @EIMD_fhl Shameless plug onsnetwork.org/eimd

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Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?

Steven Roberts School of Aquatic and Fishery Sciences robertslab.info @sr320

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Shameless plug

  • nsnetwork.org/eimd

@EIMD_fhl

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Shameless plug

  • nsnetwork.org/eimd

@EIMD_fhl

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Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?

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Open Science

  • You are free to Share!
  • Our lab practices open notebook

science

  • Data, Preprints, Proposals, Slidedecks available

FigShare, GitHub, and lab website (robertslab.info)

These slides plus links @ robertslab.info

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commercially important traits

Lab Background

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commercially important traits

modified from Lab Background

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modified from

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modified from

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modified from

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Today

modified from 1 2 3

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Histone Modification Epigenetics short RNAs DNA Methylation

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Photo credit: Flickr, Creative Commons, he-boden

Epigenetics

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Epigenetics

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m m DNA Methylation

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Function?

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Non- Vertebrates?

landscape and function is very different than what is observed in vertebrates

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Non- Vertebrates?

landscape and function is very different than what is observed in vertebrates

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Non- Vertebrates?

landscape and function is very different than what is observed in vertebrates

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Absent in several model

  • rganisms
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Function? Oysters?

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mosaic

associated with gene bodies

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Roberts and Gavery 2012

in silico analysis

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Roberts and Gavery 2012

in silico analysis

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Roberts and Gavery 2012

in silico analysis

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Two Lineages

  • Sperm +

Larvae (72h & 120h) Claire Olson Whole Genome BS-Seq

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Claire Olson

Differentially Methylated Loci predominant in Transposable Elements

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Claire Olson

github.com/sr320/olson-dev

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Summary

  • Sparsely (~16 %) methylated genome
  • Gene body methylation
  • Function specific
  • TEs are not hypermethylated across

genome

  • Preliminary evidence indicates DMRs

are predominant in TEs

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Role?

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In species that experience a diverse range of environmental conditions, processes have evolved to increase the number of potential phenotypes in a population in order to improve the chances for an individual’s survival.

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Roberts and Gavery 2012

in silico analysis

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Stochastic Variation housekeeping response to change

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Mackenzie Gavery

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housekeeping response to change

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Targeted Regulation

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Mackenzie Gavery

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Mackenzie Gavery

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Transposable Elements providing increased diversity?

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modified from Beginning to test these Hypotheses

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Very new data Environment and gene expression stochastic or targeted?

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Very new data Environmental impact (Estrogens)

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Very new data Ocean Acidification Selection Environmental Impact Katie Lotterhos

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Very new data Heritability Plasticity Local Adaptation Genetics versus Epigenetics

Common Garden Experiment

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Acknowledgements Mackenzie Gavery Claire Ellis Sam White Bill Howe Dan Halperin

EPA

STAR

DNA methylation

robertslab.info slides, data & more @

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