DNA D DNA Double bl Helix DNA stands for: DNA stands for: U d - - PowerPoint PPT Presentation

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DNA D DNA Double bl Helix DNA stands for: DNA stands for: U d - - PowerPoint PPT Presentation

DNA D DNA Double bl Helix DNA stands for: DNA stands for: U d Under a Deoxyribose microscope Nucleic DNA looks like DNA looks like Acid a twisted up ladder! Every living thing has DNA. That means you have something in common


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DNA D bl DNA Double Helix

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DNA stands for:

U d

DNA stands for:

  • Deoxyribose
  • Nucleic

Under a microscope DNA looks like

  • Acid

DNA looks like a twisted up ladder! Every living thing has DNA. That means you have something in common with a shark a have something in common with a shark, a flower, a mushroom, and a horseshoe crab!

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Add mitochondrian

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Adenine can only pair with Thymine Adenine can only pair with Thymine Guanine can only pair with Cytosine

Green can only go with Red Purple can only go with Yellow

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Everyone knows we can tell people apart by their fingerprints… th r f ng rpr nts…

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But today we are going to look

Lead into Sea Turtle CSI Scenario description

But today we are going to look more at footprints than fingerprints!

Scenario description Sea Turtle crawl, Dauphin Island

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Scenario

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Mitochondria are structures within cells that convert the energy the energy from food into a form h ll that cells can use.

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  • Only inherited from the mother
  • Natal Homing – female sea turtles

return to the beach where they were return to the beach where they were born

  • Beach footprint – imprint on DNA
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Thymine Guanine Adenine Cytosine

How do we get enough DNA to test this scenario, to find out where that sea turtle meat came from?

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Common CSI and Cop show practice: Common CSI and Cop show practice:

Not enough DNA is gathered to run a Gel Electrophoresis!

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http://users.ugent.be/~avierstr/principles/pcr.html

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Step #1 Denat ration heat p to separate Step #1: Denaturation – heat up to separate

Make 2 separate strands!

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Step #2: Annealing: make primers

3’ 5’

Primer

5’

Primer

5’ 5’ 3’

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Step #3: Extension: G th DNA

3’ 5’

Grow the DNA molecules by adding nucleotides! adding nucleotides!

5’ 5’ 5’ 3’ 5’

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Last Step: Match your DNA to known Haplotype

Haplotype A North Carolina South Carolina Georgia Georgia Florida

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Bio Edit program

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Online virtual lessons & interactive games

Genetic Science Learning Center, The University of Utah

  • http://learn.genetics.utah.edu/content/labs/extraction/
  • http://learn.genetics.utah.edu/content/labs/pcr/
  • http://learn.genetics.utah.edu/content/labs/gel/

http://www.nobelprize.org/educational/ medicine/dna_double_helix/about.html Interactive “matching” game! g g

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