SUN UN Nanomaterials in a perspective of effects on ecosystem - - PowerPoint PPT Presentation

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Sustainable Nanotechnologies

SUN UN

Nanomaterials in a perspective of effects on ecosystem services and ecological risk

Presented by: Janeck J Scott-Fordsmand (AU)

Wor

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by: y: JJ Sc Scott-Fordsmand (AU) , E , E Simensin (UNIV IVIE IE ), T , T F Fernandes (HWU) , J JM N Navas,( ,(IN INIA), , R Hand ndy ( (UoP), TFM Roelofs (VUA), H Hund und-Rinke ke (IM IME), M MJB Amor

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(UAVR).

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ECOSYSTEM SERVICES

Clean water – Sewage sludge function Food production – Soil-, water-function, trophic transfer Biodiversity – Species sensitivity, loss Gene Pool – Epigenetic etc

ECOLOGICAL RISK

Ecology beyond directly serving to human mankind

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TARGETS IN SUN

Develo lop t tools ls to e

• estima

mate long ng-term e effects inclu luding repe peated e expos posures, focusing on bio-accumulation, secondary poisoning and trophic transfer Develo lop lo long-term predict ctive t tools ba based on n short-term expe perime ments using gene expression HTP/-content tests tools Develo lop r risk e k evalu luation tools ls for l long-term s rm scenari rios (and suggest risk mitigation approaches), including scenarios with multiple stressors

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EX EXAMP AMPLES LES

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INITIAL S SCR CREE EENING G

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INITIAL SCREENING

Protocol optimization for dispersion. Characterization of dispersed NMs in test medium Interference of NMs with measurements e.g. cytotoxicity assays. In vivo and In vitro cytotoxicity tests

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INITIAL SCREENING

NM characterisation in test medium

TE TEM M WCCo CCo NP P TE TEM M Org Pi Pig Red TE TEM F M Fe2O3 Pi Pig ED EDX WCCo Co NP P ED EDX Org Pi Pig Red EDX Fe X Fe2O3 Pi Pig DLS DLS WCCo Co NP NP DLS DLS Org Pi Pig Red DLS DLS F Fe2O3 Pi Pig TE TEM M Cu CuO NP P TE TEM M MW MWCNTs Ts ED EDX Cu CuO NP P ED EDX MWCNTs Ts DLS DLS Cu CuO NP NP DLS DLS MWCNTs Ts

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TESTs IN VITRO AND IN VIVO

CuO NM Toxicity in Cell line Assay RTL-W1

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TESTs IN VITRO AND IN VIVO

CuO NM Toxicity in single species test

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TESTs ON SLUDGE ACTIVITY

Simulation in model wastewater treatment plants

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LONG T G TERM M AN AND R REP EPEATED EXPOSU SURE T TOOLS OOLS

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LONG TERM VS REPEATED

CuO inhibition of microbial respiration

Blue single exposure /Yellow repeated exposure

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FOOD CHAIN - TROPHIC TRANSFEER

Mult lti- sp spec ecies

Predato tor Pray ay Competi titon

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Mic icroorga ganism Mut utua ualism Decom

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r

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LONG ONG-TERM P M PREDICTIONS BASED ON ON SHOR HORT-TERM TERM EXPER ERIMENTS

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IN VITRO BASE FOR PREDICTION

CuO and MWCNTs inhibition in in vitro assays

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IN VIVO BASE FOR PREDICTION

NMs inhibtion in in vivo enchytraeid tests

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• F. candida
• E. crypticus

EPIGENETIC PREDICTION

• E. crypticus & F. candida multigeneration

Survival, Reproduction, Epigenetics, Gene expression

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RISK K EVALUATION T N TOOLS OOLS FOR OR LONG ONG-TERM M SCENA NARIOS OS

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Species sensitivity distributions

HAZARD PREDICTION

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WHAT SUN IS DELIVERING

TOOL OOLS

In vivo tools estimating long-term risks of nanomaterials in vitro and rapid high-throughput content tools to estimate long-term consequences Integrated probabilistic based risk evaluation

Tool

• ols a

and dat ata t a tar argeted s scenar arios r relevan ant and i nd important eco ecosystem em ser ervices