/ SUN UN Nanomaterials in a perspective of effects on ecosystem services and ecological risk Presented by: Janeck J Scott-Fordsmand (AU) Wor ork b by: y: JJ Sc Scott-Fordsmand (AU) , E , E Simensin (UNIV IVIE IE ), T , T F Fernandes (HWU) , J JM N Navas,( ,(IN INIA), , R Hand ndy ( (UoP), TFM Roelofs (VUA), H Hund und-Rinke ke (IM IME), M MJB Amor orim ( (UAVR). Sustainable Nanotechnologies
/ ECOSYSTEM SERVICES Clean water – Sewage sludge function Food production – Soil-, water-function, trophic transfer Biodiversity – Species sensitivity, loss Gene Pool – Epigenetic etc ECOLOGICAL RISK Ecology beyond directly serving to human mankind
/ TARGETS IN SUN Develo lop t tools ls to e o estima mate long ng-term e effects inclu luding repe peated e expos posures, focusing on bio-accumulation, secondary poisoning and trophic transfer Develo lop lo long-term predict ctive t tools ba based on n short-term expe perime ments using gene expression HTP/-content tests tools Develo lop r risk e k evalu luation tools ls for l long-term s rm scenari rios (and suggest risk mitigation approaches), including scenarios with multiple stressors
/ EX EXAMP AMPLES LES
/ INITIAL S SCR CREE EENING G
/ INITIAL SCREENING Protocol optimization for dispersion. Characterization of dispersed NMs in test medium Interference of NMs with measurements e.g. cytotoxicity assays. In vivo and In vitro cytotoxicity tests
/ INITIAL SCREENING NM characterisation in test medium DLS MWCNTs DLS Ts TE TEM M Cu CuO NP P EDX Cu ED CuO NP P DLS DLS Cu CuO NP NP TE TEM M MW MWCNTs Ts ED EDX MWCNTs Ts ED EDX WCCo Co NP P TE TEM M WCCo CCo NP P DLS DLS WCCo Co NP NP TEM TE M Org Pi Pig EDX Org Pi ED Pig Red DLS DLS Org Pi Pig Red Red DLS F DLS Fe 2 O 3 Pi Pig TEM F TE M Fe 2 O 3 Pi Pig EDX Fe X Fe 2 O 3 Pi Pig
/ TESTs IN VITRO AND IN VIVO CuO NM Toxicity in Cell line Assay RTL-W1
/ TESTs IN VITRO AND IN VIVO CuO NM Toxicity in single species test
/ TESTs ON SLUDGE ACTIVITY Simulation in model wastewater treatment plants
/ LONG T G TERM M AN AND R REP EPEATED EXPOSU SURE T TOOLS OOLS
/ LONG TERM VS REPEATED CuO inhibition of microbial respiration Blue single exposure /Yellow repeated exposure
/ FOOD CHAIN - TROPHIC TRANSFEER Predato tor Pray ay Decom ompos ose r Mult lti- spec sp ecies Mut utua ualism Competi titon on Mic icroorga ganism
/ LONG ONG-TERM P M PREDICTIONS BASED ON ON SHOR HORT-TERM TERM EXPER ERIMENTS
/ IN VITRO BASE FOR PREDICTION CuO and MWCNTs inhibition in in vitro assays
/ IN VIVO BASE FOR PREDICTION NMs inhibtion in in vivo enchytraeid tests
/ EPIGENETIC PREDICTION E. crypticus & F. candida multigeneration Survival, Reproduction, Epigenetics, Gene expression F. candida E. crypticus
/ RISK K EVALUATION T N TOOLS OOLS FOR OR LONG ONG-TERM M SCENA NARIOS OS
/ HAZARD PREDICTION Species sensitivity distributions
/ WHAT SUN IS DELIVERING TOOL OOLS In vivo tools estimating long-term risks of nanomaterials in vitro and rapid high-throughput content tools to estimate long-term consequences Integrated probabilistic based risk evaluation Tool ools a and dat ata t a tar argeted s scenar arios r relevan ant and i nd important eco ecosystem em ser ervices
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