Comparison of Planarian ( Dugesia tigrina ) Regeneration Rate in - - PowerPoint PPT Presentation

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May 13, 2023 297 likes •422 views

Comparison of Planarian ( Dugesia tigrina ) Regeneration Rate in Caffeine and Aloe vera Solutions Diane Kwon and Joel Tatum Biology 3B Department of Biological Sciences Saddleback College Mission Viejo, CA 92692 Planarians (Dugesia tigrina) o

SLIDE 1

Comparison of Planarian (Dugesia tigrina) Regeneration Rate in Caffeine and Aloe vera Solutions

Diane Kwon and Joel Tatum Biology 3B Department of Biological Sciences Saddleback College Mission Viejo, CA 92692

SLIDE 2

Planarians (Dugesia tigrina)

Planarians regenerate after cut
279 cuts
2 new planarians identical

to original planarian

Same memories
Planarians sensitive to

concentrated solutions

Spring water only
Tap and DI water may kill

planarians

SLIDE 3

Caffeine and Aloe vera

Caffeine
Stimulant
May inhibit cell growth
Aloe vera
Succulent plant
Stimulate cell growth

and healing

Mucilage tissue (Aloe

vera gel)

SLIDE 4

Hypothesis

Planarian regeneration rate will decrease in caffeine and Aloe vera solutions compared to that of spring water.

SLIDE 5

Materials and Methods

Planarians purchased from Carolina

Biological Supplies (n=30)

3 solutions
Arrowhead spring water (control)
Caffeine (0.00001 M)
Aloe vera (0.00019%)
Planarians fed 3 days before

experiment but not during

Proportion-readjustment

regeneration

Cells self-destruct to provide

energy/nutrients for regeneration

SLIDE 6

Materials and Methods

Transverse cut anterior to

pharynx

Observed transected tail

portion

Head development more

distinct

Experiment duration - 23 days
Oct 26, 2015 to Nov 18, 2015

SLIDE 7

Planarian Regeneration

1. Stage 1: wound healing

Neoblasts: population of

stem cells

2. Stage 2: blastema formation
Cell proliferation

1 2

SLIDE 8

Planarian Regeneration

3. Stage 3: growth 4. Stage 4: differentiation 3 4

SLIDE 9

Image from: http://www.extremetech.com/extreme/161092-decapitated-worms-can-regenerate-their-brains-and-the-memories-stored-inside

SLIDE 10

Results

Single-factor ANOVA

Bonferroni Correction post-hoc test

No significant difference

(p=0.0726)

SLIDE 11

Discussion

Caffeine diverts energy from regeneration
Healing properties of caffeine and Aloe vera in lower concentrations
Below lethal limit
Potential for growth stimulation
Sensitivity of planarians to higher concentration of solutes
Inconsistent regeneration rate through stages of regeneration

SLIDE 12

Acknowledgments

Thank you, Professor Teh, for supplies and guidance. Thank you, Saddleback College Department of Biological Sciences, for providing necessary resources. Thank you, caffeine, for getting us through the project, even though it inhibited planarian regeneration. (Poor little guys…)