cell HIV receptor (CD4) - PDF document

Co-receptor diagnosis – the molecular perspective Corus: Co-receptor usage as a marker for specific HIV diagnostics with high sensitivity 1 � Daniel.Hoffmann@uni-due.de 2008 c HIV–cell interaction necessary for entry � � � � � � co−receptor (CCR5 or CXCR4) cell � � HIV �� �� � � �� �� � � receptor (CD4) ��� ��� � � ��� ��� � � V3 ��� ��� gp120 2 � Daniel.Hoffmann@uni-due.de 2008 c

Therapeutic blocking of co-receptor ◮ Interaction of co-receptor and HIV necessary for cell-entry ⇒ Blocking of co-receptor as therapy! ◮ If HIV uses CXCR4: do not use CCR5-blockers! ⇒ What co-receptor is used by HIV in patient? ⇒ Specific diagnosis! Moreover: ◮ early diagnosis of X4 ◮ diagnosis with complex quasi-species mixtures of X4/R5 3 � Daniel.Hoffmann@uni-due.de 2008 c Idea for diagnostic assay R5 R5 R5 X4 R5 R5 X4 X4 R5 R5 "X4" ACGGGCA... R5 X4−virus R5 binding amplification, X4 peptide sequencing, affinity diagnosis sequence analysis blood sample separation 4 � Daniel.Hoffmann@uni-due.de 2008 c

Core of corus: the peptides 5 � Daniel.Hoffmann@uni-due.de 2008 c Acknowledgement ◮ BMBF/DLR for funding in programme “Molecular Diagnostics” ◮ Partners: ◮ Rolf Kaiser and group, Inst. for Virology, U Cologne ◮ Stefan Reuter (Mark Oette) and group, University Clinic Düsseldorf ◮ Jutta Eichler and group, Helmholtz Zentrum für Infektionsforschung, Braunschweig ◮ Ursula Dietrich and group, Georg-Speyer-Haus, Frankfurt ◮ Siemens Diagnostics ◮ U Duisburg-Essen, esp. Niko Dybowski & Manuel Prinz 6 � Daniel.Hoffmann@uni-due.de 2008 c