cell HIV receptor (CD4) - - PDF document

Co-receptor diagnosis – the molecular perspective

Corus: Co-receptor usage as a marker for specific HIV diagnostics with high sensitivity

1 c Daniel.Hoffmann@uni-due.de 2008

HIV–cell interaction necessary for entry

(CCR5 or CXCR4) receptor V3

• cell

co−receptor gp120 (CD4)

HIV

• 2

c Daniel.Hoffmann@uni-due.de 2008

Therapeutic blocking of co-receptor

◮ Interaction of co-receptor and HIV necessary for cell-entry

⇒ Blocking of co-receptor as therapy!

◮ If HIV uses CXCR4: do not use CCR5-blockers!

⇒ What co-receptor is used by HIV in patient? ⇒ Specific diagnosis! Moreover:

◮ early diagnosis of X4 ◮ diagnosis with complex quasi-species mixtures of X4/R5

3 c Daniel.Hoffmann@uni-due.de 2008

Idea for diagnostic assay

R5 R5 R5 R5 R5

X4 X4

R5

X4−virus binding peptide affinity separation amplification, sequencing, sequence analysis

X4 X4

R5 R5 R5

ACGGGCA... blood sample "X4" diagnosis

4 c Daniel.Hoffmann@uni-due.de 2008

Core of corus: the peptides

5 c Daniel.Hoffmann@uni-due.de 2008

Acknowledgement

◮ BMBF/DLR for funding in programme “Molecular

Diagnostics”

◮ Partners:

◮ Rolf Kaiser and group, Inst. for Virology, U Cologne ◮ Stefan Reuter (Mark Oette) and group, University Clinic

Düsseldorf

◮ Jutta Eichler and group, Helmholtz Zentrum für

Infektionsforschung, Braunschweig

◮ Ursula Dietrich and group, Georg-Speyer-Haus, Frankfurt ◮ Siemens Diagnostics ◮ U Duisburg-Essen, esp. Niko Dybowski & Manuel Prinz 6 c Daniel.Hoffmann@uni-due.de 2008