Bob Goldberg MCDB Retreat December 6, 2019 Dissecting Early Plant Embryo Development
UCLA Plant & Agriculture History
Aerial Photograph of UCLA in 1929 There Were 18,000 Farms in Los Angeles County in 1929!!! From 1901 to 1950 Los Angeles County Was the Largest Agricultural Producing County in the US!!! Bel- Beverly Farms!! Air Hills Hilgard Blvd. Sunset Blvd. Westwood Blvd. Original Agricultural College and Citrus/Avocado Orchard Thelner Hoover Los Angeles Population = 1,238,000 4/11/29
A Sample of LA County Agriculture in the 1930s 600,000 Acres ~30% of LA County Total Area!! Cash Value of $3.1B in 2019 Dollars!!! Crop Acreage Trends For LA County & Southern California, 1925-1954, Published by LA County Board of Supervisors, Compiled by LA County Chamber of Commerce UCLA Library OCLC21700378
Original UCLA College of Agriculture-1929 Kinsey Hall - Agricultural College (Now Humanities Bld.)
Professor Jacob Biale (1902-1989)
Avocado Rootstock Progeny Nursery on the UCLA Campus in 1936 Plant Sciences 106 Fruit Physiology and Storage Problems CA Schroeder, Cal. Avocado Year Book, 1992
Legacy of UCLA Plant Sciences Avocado Research
What Happened to the UCLA College of Agriculture? Aerial Photograph of UCLA in 2008 Bel-Air Beverly Hills UCLA Medical Center - 1955 Former Farms Sunset Blvd. Hilgard Blvd. Westwood Blvd. 11/17/12
Botany Building Designed By Paul Williams Built for $859,000 and Opened in 1959! Bel-Air Beverly Hills Former Farms Sunset Blvd. New Botany Department Hilgard Blvd. Professor & Chair Jacob Biale (1902-1989) Professor Sam Wildman (1912-2004) Professor Bernie Phinney (1917-2009) Original Agricultural College and Citrus/Avocado Orchard Westwood Blvd. 11/17/12
Ninety Three Years of Plant Research at UCLA Bel-Air Beverly Hills Former Farms Sunset Blvd. Hilgard Blvd. Original Agricultural College and Citrus/Avocado Orchard Agriculture Botany Biology Molecular Cell & Developmental Biology 1927-1955 1955-1972 1972-1995 1995 to Present
So……Why Study Seeds?? Water Animals Wind Seeds Protect and Disperse Plant Embryos and Come in Many Shapes and Sizes!
Our Food is Derived From Fifteen Crops & Over Half Produce Seeds For Human and Animal Consumption Non-Seed Crops Seed Crops • Potato • Wheat • Sweet Potato • Rice • Cassava • Corn • Sugar Beet • Barley • Sugar Cane • Sorghum • Banana • Soybean • Common Bean • Coconut • Canola In Some World Populations 75% of Calories Are Derived From Seeds!
Bean Embryo Shortly After Fertilization Post-Fertilization Next Plant Generation Embryo Axis Embryo Mature Embryo Proper Cotyledon Consumed During Suspensor Germination Provides Nutrients & Growth Suspensor Morphological Diversity Regulators Degenerates During Seed Development
Using Comparative Genomics To Uncover Regulatory Networks Programming Suspensor Development Scarlet Common Soybean Arabidopsis Runner Bean Bean Embryo Proper Suspensor Before LCM After LCM Laser Capture Microdissection (LCM) RNA-Seq ChIP-Seq Using Leica LMD System
Identification of Suspensor Transcription Factors That Are Up-Regulated in All Four Plant Species After Fertilization Scarlet Common Runner Bean Bean Soybean Arabidopis Suspensor (SUS) Embryo proper (EP) EP EP SHARED Suspensor- Specific TFs SUS SUS • WOX8/9 • ZFN1 • ARF16 • AGL11 LCM • WRKY6 • AGL9/SEP3 RNA-Seq Profile of EP & SUS • HDG11 • SRS5 • FEZ • ANAC087 • C2C2-Dof TF • ANAC002 • ARF10 • ERF/AP2 TF Suspensor >5x Up-Regulated TFs • CDF3 • GATA9 • WIP2/NTT • C2H2 TF • HB17 WOX8/9 Regulates The Development Of The Basal Scarlet Runner Bean Arabidopsis Embryo Lineage RNAseq In-Situ In-Situ RNAseq Apical EP EP fate SUS SUS ep Basal sus fate AtWOX9 PcWOX9 (Development,2003) (Modified from Developmental Cell,2011)
Does WOX9 Target SHARED Suspensor Up- Regulated TFs? Scarlet Chromatin Runner Bean Soybean ChIP-Seq Immunoprecipitation Libraries (ChIP) 0.5 mm 1 mm WOX9 WOX9 antibody ChIP-Seq with WOX9 antibody WOX9 Gene/TF 305 Genes 828 Genes (32 TFs) (99 TFs)s Chromatin WOX9 Target Genes SHARED Suspensor- Specific TFs Targeted Any WOX9 Target Genes??? by WOX9 • WOX8/9 • ZFN1 ❖ • ARF16 • AGL11 YES! ❖ ❋ ❋ • WRKY6 • AGL9/SEP3 ❖ ❋ ❋ • HDG11 • SRS5 ❋ ❖ ❋ • FEZ • ANAC087 ❖ ❋ v 6 TFs in Scarlet Runner Bean • C2C2-Dof TF • ANAC002 ❖ ❋ • ARF10 • ERF/AP2 TF ❋ ❋ 14 TFs in Soybean • CDF3 • GATA9 ❋ • WIP2/NTT • C2H2 TF • HB17 ❋
Gaining Entry Into the Regulatory Network Controlling Suspensor Differentiation & Function WOX9 Weterings, Plant Cell, 2001 WOX9 SRB At WOX9 WOX9 WOX9 WOX9 WOX9 WRKY6 ARF16 FEZ HDG11 ARF10 Suspensor Differentiation & Function
Many Thanks To….. John Harada Min Chen Julie Pelletier Jer-Young Lin Xiaomeng Wu Kelli Henry Brandon Le Anhthu Bui Lauren Bowman Matteo Pellegrini One Final Comment……
Honors Collegium 70A Genetic Engineering in Medicine, Agriculture, and Law
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