BioART & Colr Coli presented by iGEMOSAKA @ iGEM 2009 MIT - - PowerPoint PPT Presentation

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bioart amp colr coli presented by igemosaka igem 2009 mit
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BioART & Colr Coli presented by iGEMOSAKA @ iGEM 2009 MIT - - PowerPoint PPT Presentation

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BioART & Colr Coli presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th Introduction BioART 1933 - Alexander Fleming Germ Painting 2000 - Eduardo Kac GFP Bunny BioART beyond the aesthetics

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BioART & Colr Coli

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Introduction

BioART

1933 - Alexander Fleming “Germ Painting” 2000 - Eduardo Kac “GFP Bunny”

BioART beyond the aesthetics

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Object

BioART BioSCIENCE

Making biosystem as biomedia ・Color ・Signal ・Motility ex) Making art works by using biomedia ・New tool ・New expression ・New ethics ex) Request Stimulus

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Bio media

COLOR SIGNAL MOTILITY

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

COLOR

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Pigment

Color

Fluorescent protein

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

GREEN

GFP

RED

RFP

YELLOW

YFP

CYAN

CFP

ORANGE

mOrange

Color : Fluorescent1

Fluorescent protein’s color p(lac)

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Color : Fluorescent2

RED GREEN CYAN

GFP RFP

YELLOW

YFP CFP

ORANGE

mOrange

Fluorescent protein’s color p(Tet)

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Color : Pigment

Carotenoid biosynthesis pathway

Lycopene ß-Carotene Xanthophyll Phytoene CrtB GGPP CrtE FPP CrtI CrtY CrtZ

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Color : Pigment

Carotenoid Orange

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Color : Future work

Japanese Traditional Colors

AKABENI HIWAMOEGI RINDOU YAMABUKI SEIRAN

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Color : Future work

Color gradation using Signal and Motility’s function

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

SIGNAL

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Signal

For Communication with Environment or Other Cells

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Signal : Quorum sensing

R gene I gene AHL R Protein I Protein Complex Activation Promoter

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Signal : Object

Sender Receiver AHL Function

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Signal : Characterization

LuxI LasI RhlI CinI LuxR LasR RhlR CinR

Sender

p(Tet) Inducer

Receiver

p(Tet) Receiver Inducible Promoter GFP

Incubated at 37˚C for 24 hours Fluorescence measured at 488/513nm

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Signal : Result

Sender - Receiver Response Fluorescence / OD

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Signal : Future work

Cross Talk

LuxI LasI Rhll CinI LuxR LasR RhlR CinR

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

MOTILITY

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Protonic Nanomachine Project, ERATO

Self assembly of rotor

Flagellar protein export apparatus

Self assembly of stator Propeller Elongation Flagellar Swimming Hook and Junction formation

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Motility : Procedure

Our Target

Previous Target Rotational Motor Flagellar Assembly! Protein secretion by flagellar export apparatus Flagellar Assembly FliH

Substrate

FliH expression in the absence of FliI

(Minamino, 2008)

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Motility : Results

Swarming Motility Flagellar Protein Export

Inhibition of Flagellar Assembly FliH

constitutive

The motility can be controlled by expressing FliH!

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

MODELING

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Cell concentration AHL concentration

Modeling : Equation

Growth rate Production rate

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Ex) Triangle model

Modeling : Simulation

Each cell regulates others as circulation

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Modeling : Signal and Motility

Distance Distance Cell Concentration Cell Concentration

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Without regulation With regulation

Modeling : Results

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Summary

  • 1. Assembled 10 fluorescence protein parts for germ painting.
  • 2. Assembled 1 pigment parts for germ painting.
  • 3. Devised color gradation systems and developed simulation for it.
  • 1. Assembled 4 senders and 4 receivers parts.
  • 2. Characterization of 4×4 combination of sender-receiver parts.
  • 3. Modeling the cell motility and development of simulation of triangle model.
  • 1. Developed a new approach to control the motility.
  • 2. Construction and characterization new parts, FliH.
  • 3. Confirmation of expected effect of our parts on swarming motility and protein export.

COLOR SIGNAL MOTILITY

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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Human Practice

Bio Art Synthetic Biology

Artistic usage of biotechnology will open the potential of it. Bio art can put the forefront science in front of the public. Bio art raise many bioethical issues that are hidden in social system. To say that “what is life” , collaboration

  • f scientist and artist must be needed.
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presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th

Thank you!!

To see our works, ・Poster: Lobby 13, B38 on Sunday ・Web Gallery: www.microbialart.com ・E-mail: igemosaka@gmail.com