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Design Task Museum! Museum!

Design  step1 ： Define the city  step2 ： Build a database for the city

Design How to build a bio-database

Design Specific enzyme cutting sites

Design  step3 ： Choose the data  step4 ： Find every possible way  step5 : Find best way

Design How to find every possible routes? Choosen City library 1 2 3 4 5 6 7 8 Choosen Path library

Design Obtain the specific results of pathway  Electrophoresis  DNA sequencing

Design Bio-CPU

Design Logical principles

Design Logical principles BBa_K1341000

Design The advantage of LTT promoter BBa_K1341000 BBa_K091101 The x and y axes form a grid of 36 pairs of inducer concentrations. The concentrations tested were 0, 1, 10, 50, 100, and 200 ng/mL for aTC and 0, 0.001, 0.002, 0.005,0.010, 0.100, and 1.000mM for IPTG behavior is depicted 6 h after induction.

Design Step 1 BBa_K1341001 No.5 Without No.5 Fluorescenc 1 0 e output

Design Step 2 No.5 N0.5&No.8 Fluorescence 1 0 output

Design Step 3 No.5&N0.2&N0.8 N0.5&No.8 Fluorescence 0 0 output

Design Why add IPTG first? Fluorescence No.5&N0.2&N0.8 N0.5& N0.5& output No.8 No.2 Add IPTG 0(green) 1 0 Add aTc 1(red) 0 1 Conditions are ± 100 ng/mL aTc and ± 1mM IPTG, All data is obtained 6 h after induction.

Design Step 4 Add IPTG No.5&N0.2&N0.8 N0.5&No.8 Fluorescence 0 1 output

Design Step 5 Add No.5&N0.2&N0.8 The x and y axes form a grid of 36 pairs of inducer concentrations. Behavior is aTc depicted 6 h after induction. Fluorescence 1 output

Result Result of DNA sequencing

Result Route:2-4-5-7-8 is the right result! 3 6 8 2 4 7 1 5

Human Practice& Help each other

Human Practice  College freshmen “ 50 5089 9 col ollege ge fr fres eshme hmen ”

Human Practice  Military Training

Human Practice  Established community

Human Practice  Sina Blog  APP

Human Practice  CCiC(Central China iGEMers' Consortium )

Help Each Other Communicate with Central China iGEMers' Consortium: BNU-China CAU_China HUST-China, HZAU-China Jilin_China LZU-China, NJU-QIBEBT NUDT_CHINA OUC-China, Peking SCAU-China,SCUT WHU-China WHU-Pharm Repair the debug of iGEM biobrick library: WHU-China WHU-Pharm The questionnaire survey: ETH Zurich HZAU-China LZU-China BIT,SCUT BGU_Israel Michigan

Achievements  Submit 2 well document biobricks LTT GFP switch device:BBa_K1341000 Path way test system:BBa_K1341001 Work as expected  Characterization of an existingBioBrick TTL promoter :BBa_K091101  Solving a bio-navigational probelm  Safety training  Attribute work done by the students .  Help registered iGEM team from another school.  Human practice

Thank you for listening!

P45: control plasmid copy number Relative fluorescence: GFP/luciferase

WIKI

Project

Future work Hierarchical Hierarchical Communication Communication System System