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Background Background Background Background Design Task Museum! - - PowerPoint PPT Presentation
Background Background Background Background Design Task Museum! - - PowerPoint PPT Presentation
Background Background Background Background Design Task Museum! Museum! Design step1 Define the city step2 Build a database for the city Design How to build a bio-database Design Specific enzyme cutting sites Design
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Background
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Background
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Background
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Museum! Museum! Task
Design
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- step1：Define the city
- step2：Build a database for
the city
Design
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Design
How to build a bio-database
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Design
Specific enzyme cutting sites
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- step4：Find every
possible way
- step5 : Find best way
- step3：Choose the data
Design
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1 2 3 4 5 6 7 8 Choosen City library Choosen Path library
Design
How to find every possible routes?
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Electrophoresis DNA sequencing
Design
Obtain the specific results of pathway
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Design
Bio-CPU
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Design
Logical principles
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BBa_K1341000
Design
Logical principles
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BBa_K091101
The x and y axes form a grid of 36 pairs of inducer concentrations. The concentrations tested were 0, 1, 10, 50, 100, and 200 ng/mL for aTC and 0, 0.001, 0.002, 0.005,0.010, 0.100, and 1.000mM for IPTG behavior is depicted 6 h after induction.
BBa_K1341000
Design
The advantage of LTT promoter
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BBa_K1341001
No.5 Without No.5 Fluorescenc e output 1
Design
Step 1
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No.5 N0.5&No.8 Fluorescence
- utput
1
Design
Step 2
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No.5&N0.2&N0.8 N0.5&No.8 Fluorescence
- utput
Design
Step 3
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Conditions are ±100 ng/mL aTc and ±1mM IPTG, All data is obtained 6 h after induction.
Fluorescence
- utput
No.5&N0.2&N0.8 N0.5& No.8 N0.5& No.2 Add IPTG 0(green) 1 Add aTc 1(red) 1
Design
Why add IPTG first?
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Add IPTG No.5&N0.2&N0.8 N0.5&No.8 Fluorescence
- utput
1
Design
Step 4
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Add aTc No.5&N0.2&N0.8 Fluorescence
- utput
1
The x and y axes form a grid of 36 pairs
- f inducer concentrations. Behavior is
depicted 6 h after induction.
Design
Step 5
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Result
Result of DNA sequencing
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1 2 4 5 3 6 8 7
Result
Route:2-4-5-7-8 is the right result!
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Human Practice& Help each other
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College freshmen
“50
5089 9
col
- llege
ge fr fres eshme hmen”
Human Practice
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Human Practice
Military Training
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Established community
Human Practice
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Sina Blog APP
Human Practice
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CCiC(Central China iGEMers' Consortium)
Human Practice
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Help Each Other
Communicate with Central China iGEMers' Consortium: BNU-China CAU_China HUST-China, HZAU-China Jilin_China LZU-China, NJU-QIBEBT NUDT_CHINA OUC-China, Peking SCAU-China,SCUT WHU-China WHU-Pharm Repair the debug of iGEM biobrick library: WHU-China WHU-Pharm The questionnaire survey: ETH Zurich HZAU-China LZU-China BIT,SCUT BGU_Israel Michigan
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Achievements
Submit 2 well document biobricks
LTT GFP switch device:BBa_K1341000 Path way test system:BBa_K1341001 Work as expected
Characterization of an existingBioBrick
TTL promoter :BBa_K091101
Solving a bio-navigational probelm Safety training Attribute work done by the students . Help registered iGEM team from another school. Human practice
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Thank you for listening!
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P45: control plasmid copy number Relative fluorescence: GFP/luciferase
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WIKI
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Project
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Hierarchical Hierarchical Communication Communication System System