VITRIFICATION MARTINA FILOV REPROFIT INTERNATIONAL S.R.O. - - PowerPoint PPT Presentation

THE ART of EGG VITRIFICATION

MARTINA FILOVÁ REPROFIT INTERNATIONAL S.R.O.

Vitrification - principals

• TS= 1,0M trehalose, T= 37⁰C
• DS= 0.5M trehalose

Kitazato close and open system

System &Product variety

Systems:

• 1. Fully open system – Cryotop, Cryotech, Cryolock
• 2. Open cooling and close storage system – CryotopSC, OPS

3.Semi-closed cooling – Cryohook, Vitrolife, Cryotop 4.Closed thin walled , narrow capillaries- Cryotip, Cryopette 5.Carrier tools sealed into a container - High security virtrification kit, Vitrisystems -- close

Media -- Kitazato Vitrolife Irvine Sage

Trustworthiness - Reliability

Commercial availability ≠ reliability

Validation in the Lab – satisfactory, adequate ???

Short time - 10 – 20 cases Eggs - immature (MI,GV) FTF after IVF Survival – main paramater ! poor development no blastulation

Kitazato - Close

CLOSE

• 6.11.2017- 7.2.2018

24 donors – 216 oocytes 198 warmed out Results

• unsatisfactory

( inconsistent)

Is cooling and warming rate the main factor??

The magic of making bread

Factors applying to successful vitrification

Temperature

Humidity

Loading - crucial step

Kitazato – close/open

CLOSE/OPEN - 21.2.2018- 20.3.2018 12 donors – 105 oocytes 105 warmed out

Close Open

• o/cycle

36/7

5,1

53/10

5,3

SR (%) 26/36

72%

50/53

94%

FR (%) 11/26

42%

44/50

88%

BR (%) 8/26

30%

28/50

56%

UR ICSIoo (%) 3/26

12%

14/50

28%

UR Vitri Oo (%) 3/36

9%

14/53

26%

Open system study

OPEN - 10.4.2018 68 donors – 802 oocytes 437 warmed out

Results

Pregnancy rate

Biosafety

All medical intervention means a certain risk to the patient, it is duty of the health service provider to make the right decision between the possible benefits and the chances of harm

Thank you!