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Update on Guatemala through Reduction on Post-Harvest Losses of Corn Carlos Campabadal, Ph.D. (KSU) Andreia Bianchini, Ph.D. (UNL) Guillermo Gonzalez (SHARE) ( www.reducePHL.org ) May, 2016 Improving Food Security and Food Safety of


  1. Update on Guatemala through Reduction on Post-Harvest Losses of Corn Carlos Campabadal, Ph.D. (KSU) Andreia Bianchini, Ph.D. (UNL) Guillermo Gonzalez (SHARE) ( www.reducePHL.org ) May, 2016

  2. Improving Food Security and Food Safety of Smallholders Farmers in the Western Highlands of Guatemala through Reduction on Post-Harvest Losses of Corn

  3. Guatemala Team • Dr. Carlos Campabadal, KSU (Co-leader) • Dr. Andreia Bianchini, UNL (Co-leader) • Dr. Jason Ellis, KSU (Engagement Leader) • Dr. Heather Ledger-Adams, UNL (Mycotoxin Specialist) • Alejandro Morales-Quiros, KSU (Graduate Student) • Luis Eduardo Sabillon, UNL (Graduate Student) • Rodrigo Mendoza, UNL (Graduate Student) • Elizabeth Vega and Guillermo Gonzalez , SHARE Guatemala (Project Liaison) • Walfer Martinez, SHARE Guatemala (Extension Specialist) • Ada Rocina, Consultant (Equal Gender) • Ana Silvia Colmenar de Ruiz, UVG (Professor & Liaison)

  4. More than 50% of children suffer from malnutrition Guatemala Over the next five years in Guatemala, Feed the Future aims to help an estimated 219,000 vulnerable Guatemalan women, children and family members — mostly smallholder farmers — escape hunger and poverty. More than 166,000 children will be reached with services to improve their nutrition and prevent stunting and Huehuetenango Province child mortality. (69.5% malnutrition)

  5. PHL Innovation Lab Goals • Investigate cultural, social and economic factors , with specific attention to gender issues . • Increase the quantity and quality of stored food staples and dietary diversity, along with country- specific nutrition education, thus increasing access to nutritious food and reducing under nutrition and food insecurity.

  6. Guatemala Project Goals 1. Quantification of the sustainability of corn production practices and mycotoxin presence as a key parameter for grain loss for smallholder farmers in the municipalities of Chiantla and Todos los Santos Cuchumatan (Huehuetenango).

  7. Goal 1 1. Quantification of the sustainability of corn production practices and mycotoxin presence as a key parameter for grain loss for smallholder farmers in the municipalities of Chiantla and Todos los Santos Cuchumatan (Huehuetenango). a) Determine the current grain production and post-harvest practices. ( Done ) b) Determine the current health, nutrition (pending), and hygiene practices. ( Done ) c) Understand, assess, and contribute for improvement to the current equal gender issues related to post-harvest and nutrition. (on going Ada Chavarría) d) Determine the corn toxicological content, moisture and presence of insects.(on going SHARE-UVG)

  8. Toxicological Content Study (fungi, aflatoxins, entomology and moisture content) / On going Recording information per farmer per sample sent. 26 farmers actively supporting the research project provide samples of maize and answer questionnaires regarding gender and economic subjects.

  9. Toxicological Content Analysis (fungi, mycotoxins, entomology and moisture content) / Done 92 samples sent the Universidad del Valle de Guatemala: 34 grain corn and 34 on the cob "C1" and 24 samples of corn husking "C2". "C1" Farmers who produce maize "C2" Farmers who buy maize 14 temperature and relative humidity sensors

  10. Grain Quality Survey Collecting Maize Samples

  11. Grain Quality Survey Collecting Maize Samples

  12. Grain Quality Survey Training of promoters

  13. Toxicology Analysis Grain Quality: Baseline – Moisture – Huehuetenango Region Mycotoxins - Fumonisin > 2 ppm – Insects - Aflatoxin > 20 ppb – Fungal counts Aspergillus & Fusarium presence

  14. Corn Moisture 40.0 35.0 30.0 Initial moisture content (%) Moisture content (%) 25.0 20.0 Average moisture content (%) 15.0 10.0 14% moisture content 5.0 0.0 Altitude A Altitude B Altitude C Altitude A Altitude B Altitude C CHAIN 1 CHAIN 2

  15. Toxin Levels in Corn  FDA Mycotoxin Regulatory Guidance. 2011. A Guide for Grain Elevators, Feed Manufacturers, Grain Processors and Exporters. National Grain and Feed Association Average mycotoxin exposure during harvest and throughout storage in Todos Santos and Chiantla, 2014-2015 season 45.0 14.0 40.0 12.0 35.0 Fumonisin (ppm) Aflatoxin (ppb) 10.0 30.0 Aflatoxin 8.0 25.0 (ppb) 20.0 6.0 Fumonisin 15.0 4.0 (ppm) 10.0 2.0 5.0 0.0 0.0 H St 0 St 30 St 60 St 90 St 0 St 30 St 60 St 90 Chain 1 Chain 2

  16. Toxin Exposure Levels Aflatoxin daily intake for Todos Santos and Chiantla, Aflatoxin daily intake ( μ g / kg bw * day) Corn harvesting season 2014-2015 0.45 Mean, μg 0.40 Aflatoxin/ kg bw * day 0.35 (men) 0.30 Mean, μg 0.25 Aflatoxin/ kg bw * day 0.20 (women) 0.15 0.001 μ g 0.10 Aflatoxin/ kg bw * day 0.05 0.00 Altitude A Altitude B Altitude A Altitude B Altitude B Altitude C Altitude A Altitude B Altitude C Chain 1 Chain 2 Chain 1 Chain 2 Chiantla Todos Santos

  17. Toxin Exposure Levels Fumonisin daily intake for Todos Santos and Chiantla, Fumonisin daily intake ( μ g / kg bw * day) Corn harvesting season 2014-2015 180.00 Mean, μg Fumonisin/ 160.00 kg bw * day 140.00 (men) 120.00 Mean, μg 100.00 Fumonisin/ kg bw * day 80.00 (women) 60.00 2.0 μg 40.00 Fumonisin/ kg bw * day 20.00 0.00 Altitude A Altitude B Altitude A Altitude B Altitude B Altitude C Altitude A Altitude B Altitude C Chain 1 Chain 2 Chain 1 Chain 2 Chiantla Todos Santos

  18. Results Grain Quality Mold Counts in Corn 7.0 6.0 5.0 Harvest 4.0 log(CFU/g) Storage, day 0 3.0 Storage, day 30 2.0 Storage, day 60 1.0 Storage, day 90 0.0 Altitude A Altitude B Altitude C Altitude A Altitude B Altitude C Chain 1 Chain 2

  19. Insect Analysis 0.0% Altitude C 0.0% 0.0% Chain 2 0.0% Altitude B 4.0% Ephestia kuehniella 0.0% (flour moth) 4.0% Altitude A 0.0% 0.0% Sitophilus zeamais 16.0% (maize weevil) Altitude C 8.0% 8.0% Chain 1 12.0% Tribolium sp. Altitude B 4.0% (flour beetle) 0.0% 0.0% Altitude A 0.0% 0.0% 0.0% 2.0% 4.0% 6.0% 8.0% 10.0% 12.0% 14.0% 16.0% 18.0% Incidence on farms (%)

  20. Summary Results Goal 1 • Survey of more than 200 households provided insights on the current and traditional practices used in the region • Baseline data did not indicate that storage had a significant impact in toxin production (differences were not statistically significant) • Exposure levels for population in the Highlands of Guatemala are very concerning

  21. Summary Results Goal 1 • Fungal analysis showed the presence of Aspergillus, Fusarium, Penicillium , and Chladosporium . • Mold counts were on average in the 10 5 CFU/g range • Limited issues with insect infestation • On going: Expanding baseline data by collecting additional samples outside of the farms under evaluation

  22. Guatemala Project Goals 2. Implementation of key-post-harvest techniques to improve grain drying, storage time, reduce mold growth and mycotoxin presence.

  23. Goal 2 2. Implementation of key-post-harvest techniques to improve grain drying, storage time, reduce mold growth and mycotoxin presence. a) Evaluation of their current drying systems versus utilizing “on -shelf ” technologies (current work). b) Evaluation of their current storage practices versus utilizing “on - shelf” technologies (current work). c) Develop best practices and technologies for the reduction of grain quality loss (moisture content, mold, mycotoxin, etc) (current work).

  24. Goal 2 • Current drying practices : Field Attic (accumulated heat & sun) or on top of the roof Solar: husk outside or in bags Hanging seed corn

  25. Traditional Drying Separation of “good” and “bad” corn Traditional drying in the field

  26. Goal 2 • Use “On - shelf” drying practices : STR furnace dryer Furnace type dryer AflaStop Dryers: - Shallow bed developed by UVG students Solar dryer by S4S

  27. Weather Conditions in Huehuetenango • Temperature: 0 to 25 °C • Usually very cloudy • Altitude: 1500 to 3000m

  28. January 2016 • Solar & Furnace Dryer Added a furnace to help with cloudy conditions Drying rate ≈ 0.5% per hour with shelled corn Solar dryer based on the S4S model

  29. January 2016 STR Dryer Drying rate ≈ 1.0% per hour with shelled corn

  30. Drying & Storage Grain Quality Assessment (done): – Harvest, drying, Measuring moisture content with John storage (0, 30, 60 & 90 days) Deere handheld – Extension & Universidad equipment del Valle – Guatemala Measuring ambient temperature & relative humidity conditions during storage Shelling of corn

  31. Goal 2 • Current storage practices: Metal Silo Attic on top of kitchen or room Pile with husk Outdoor in piles Hanging on a beam Bags

  32. Goal 2 • “On - shelf” storage practices : Metal Silos (0.5 to 1 MT) PICS bags Plastic Drums Grain Pro bags

  33. Goal 2 • Potential Work “On - shelf” storage practices : Improved attic with channeled smoke to control pests

  34. On-Going Storage Trials Farmers Altitude Storage Samples Data Data Technolo Loggers Loggers gies Ambient inside storage 15 3 3 129 6 9 1 1 5 11 10 • Grain dried with the traditional method and STR dryer • Use several available on-site storage technologies

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