assessment and mitigation of aflatoxins and fumonisins in
play

Assessment and Mitigation of Aflatoxins and Fumonisins in Animal - PowerPoint PPT Presentation

Assessment and Mitigation of Aflatoxins and Fumonisins in Animal Feeds in Rwanda Kizito Nishimwe 1,2 , Erin Bowers 1 , Jean de Dieu Ayabagabo 2 , Richard Habimana 2 , Samuel Mutiga 3 and Dirk Maier 1 1 Iowa State University, USA 2 University of


  1. Assessment and Mitigation of Aflatoxins and Fumonisins in Animal Feeds in Rwanda Kizito Nishimwe 1,2 , Erin Bowers 1 , Jean de Dieu Ayabagabo 2 , Richard Habimana 2 , Samuel Mutiga 3 and Dirk Maier 1 1 Iowa State University, USA 2 University of Rwanda, Rwanda 3 BecA-ILRI, Kenya 4 th Annual Innovation Platform Meeting April 2, 2019 Kigali, Rwanda Kigali, Rwanda April 26, 2018 Photo Credit Goes Here

  2. Dr. Erin Bowers, Co-PI Dr. Dirk Maier, PI Mr. Kizito Nishimwe, Co-PI Mr. Jean de Dieu Ayabagabo Mr. Richard Habimana, UR, Collaborator UR, Collaborator Dr. Samuel Mutiga, BecA-ILRI, Collaborator

  3. Dr. Dirk Maier, Lab technicians, and Enumerators

  4. MYCOTOXINS OF CONCERN Aflatoxins … • Toxic metabolites produced by a variety of molds ( A. flavus, A. parasiticus) • Among the most carcinogenic substances known Fumonisins … • Toxic metabolites produced by Fusarium spp. o Neural tube defects o involved in equine leukoencephalomalacia (ELEM; moldy maize poisoning); a devastating neurologic disease of horses caused by eating feed or hay contaminated with fumonisin mycotoxins  Humans and animals are exposed to mycotoxins by consumption of food and feeds contaminated by mycotoxins (especially maize)

  5. Sources of Aflatoxin Exposure https://paepard.blogspot.com/2018/04/increasing-awareness-of-and-concerted.html

  6. https:// agrilinks .org/aflatoxins

  7. Carry-Over of Aflatoxin B1 to Aflatoxin M1 AFB1 ---> AFM1 Aflatoxin contamination Contaminated milk with AFM1 Consumers’ exposure Consequences Low milk production

  8. Association between maternal aflatoxin EAST AFRICA REGION CONTEXT exposure during pregnancy and adverse birth outcomes Outbreak of acute (Laura et al., 2018) aflatoxicosis: more 100 deaths Aflatoxigenic aspergilli in (Lewis et al., 2005) maize grain (Ismail et al., 2003) Low level of Aflatoxin contamination in soybean (Niyibituronsa B1 and M1 contamination et al., 2017) of animal feed and milk Aflatoxin in peanut (Kang ’ ethe and Lang ’ a, (Ndung ’ u et al., 2005) 2009) Mycotoxin types in some foodstuff Maize and groundnuts Aflatoxin B1 in maize contributed to 1,847+ (Umereweneza et al., flour 514 and 158+52 HCC 2018) (Nishimwe et al., 2017) (hepatocarcinoma) cases per annum, respectively (Wambui et al., 2016) Outbreak of an acute aflatoxicosis in Tanzania during 2016 Range (10 – 51,100 ppb) Aflatoxin contamination of (Kamala et al 2013) cereal-based complementary food (Rushunju et al 2013)

  9. CONSEQUENCES OF MYCOTOXIN CONTAMINATION Inappropriate policy LACK OF DATA Impact on human and animal health Risk Impairment of Rwandan underestimated products on international market

  10. RESEARCH OBJECTIVES Assessment and Mitigation of Aflatoxin and Fumonisin in Feeds in Rwanda 2. Establish mycotoxin 1. Asses prevalence of 3. Raise awareness of 4. Provide input to lab and staff capacity aflatoxins and mycotoxin contamination regulatory framework at University of fumonisins in feeds and prevention . regarding mycotoxin policy Rwanda . + Assess presence of aflatoxin M1 in farm milk samples

  11. METHODS  Targeted Population  Area of study  Dairy farmers  All 30 districts of Rwanda Questionnaire  Criteria: Use of supplements to feed animal additional to forage  Rounds  At least 2 cows  Samples collected in 6 rounds  Poultry farmers  Feed processors  Period  Feed vendors  March – October 2017  Grain (maize) mills  Methods of analysis  Feed samples: ELISA (HELICA, USA)  Milk samples: Fluorometry (VICAM, USA)  Methods validation  External validation: BecA-ILRI  Internal validation: Use of Reference Materials (Romer Labs, Austria)

  12. Dairy Producer

  13. Poultry Layer Producer

  14. Local Feed Manufacturer

  15. Commercial Feed Manufacturer

  16. Feed Vendors

  17. Feed Vendors

  18. Maize Miller

  19. OBJECTIVE 2: ESTABLISH MYCOTOXIN LAB & STAFF CAPACITY AflaTest Basic Equipment Microplate reader for ELISA analysis Romer Mill Grinder Sample cost: $15 - $25 vs >$150 HPLC Sample Cost Established mycotoxin lab with rapid mycotoxin tests at U of Rwanda Department of Food Science & Technology for research and extension and trained staff to operate it

  20. OBJECTIVE 3: RAISE AWARENESS OF MYCOTOXIN CONTAMINATION AND PREVENTION • Teaching materials (in Kinyarwanda) developed to raise awareness among farmers during the last (6 th ) round • Poster (in Kinyarwanda) displayed at sector/district level raising mycotoxin awareness in general public • Seminars and trainings organized at University of Rwanda

  21. Training on mycotoxin analysis (Aug 2017) Enumerators teaching farmers about mycotoxins using teaching materials Seminar at University of Rwanda (Jan 2017)

  22. OBJECTIVE 4: PROVIDE INPUT TO REGULATORY FRAMEWORK ON MYCOTOXIN POLICY • Rwanda Standards Board (RSB) develops and maintains official standards • Found one RSB standard regarding mycotoxin regulation • Cattle feed supplements — Specification: RS 100: 2017

  23. RECOMMENDATIONS AND SCALING PLANS • Feed formulation o Explore potential of mycotoxin binders in feed rations o Blending with clean grain or other ingredients o Considering different species sensitivity and age • Awareness / Education o Develop & implement training for dairy/poultry farmers, feed processors/vendors, maize millers including ingredient quality & management, feed ration formulation, mycotoxin mitigation o Deliver awareness reminders via communication channels (radio, TV, social media, phone…) o Dry and store feeds properly

  24. RECOMMENDATIONS AND SCALING PLANS (2) • Mycotoxin Testing Service o Mycotoxin lab to receive & analyze feed/ feed ingredient samples o Promote availability of mycotoxin testing service  Cost effectiveness • Intervention / Mitigation o A year-round surveillance and early detection system in the Rwanda feed value chain o Risk-based policies and standards for different species and mycotoxins in feed ingredients and mixed feeds o Initiate collaboration among the different stakeholders (Public, Academia and Private sectors)

  25. TAKE-HOME MESSAGE • Commercial feeds are highly contaminated with aflatoxins. More research needed on forages like grass and hay. • Maize bran is the major feed ingredient used and primary cause of aflatoxin presence in feeds • Low level of aflatoxin awareness among producers • Lack of comprehensive mycotoxin standards for grains, ingredients and feeds • Mycotoxin lab using ELISA (grains, ingredients, feeds) and Fluorometry (milk) can be used for low cost analysis and quick results

  26. ACKNOWLEDGMENTS • LSIL – University of Florida • Partner Institutions o University of Rwanda, Rwanda o Iowa State University (ISU), USA o BecA-ILRI, Kenya • Participants • Borlaug Higher Education for Agricultural Research and Development (BHEARD) Program • Enumerators

  27. www.feedthefuture.gov

Download Presentation
Download Policy: The content available on the website is offered to you 'AS IS' for your personal information and use only. It cannot be commercialized, licensed, or distributed on other websites without prior consent from the author. To download a presentation, simply click this link. If you encounter any difficulties during the download process, it's possible that the publisher has removed the file from their server.

Recommend


More recommend