Update for the Policy Group Meeting EM rootstock club Felicidad - - PowerPoint PPT Presentation

Update for the Policy Group Meeting EM rootstock club

Felicidad Fernández Fernández

East Malling Rootstock Club - update

• Update on pest and disease screening
• Update on Marker Assisted Selection test
• Discuss set up for next pear trials
• Incorporation of EUFRIN trials to EMRC

Pest and Disease screening 2016

• Canker (Neonectria ditissima):

– Cut shoot test in growth cabinet for advanced selections – Same genotypes have been grafted and will be planted out in high-inoculum grower sites (AHDB)

Pest and Disease screening 2016

• Canker (Neonectria ditissima):

Pest and Disease screening 2016

• Canker (Neonectria ditissima):

Pest and Disease screening 2016

• Canker (Neonectria ditissima):

5 yrs BBSRC IPA project awarded – starts Jan 2017

– Understand the mechanism of the resistance(s) in scion and rootstock germplasm – Improve characterisation of pathogen (sequencing) – Develop high-throughput screening methods for germplasm – ID new sources of resistance and examine inheritance – Identify molecular markers for MAB

Pest and Disease screening 2016

• Canker (Neonectria ditissima):

– Looking at segregations in some rootstocks populations – Separate germination from breeding seed lots – GH experiement:

• Sep – Nov 2016
• 20 seedlings from each cross
• Choose possible mapping pop.

Female FB resistance Male FB resistance M587 C.G.202 R5 resistant x AR295-6 R5 medium tolerance 98 25 Neonectria ditissima M589 Evereste Ev resistant x C.G.30 R5 resistant 100 77 Neonectria ditissima M597 Evereste Ev resistant x C.G.202 R5 resistant 100 88 Neonectria ditissima M598 Evereste Ev resistant x AR295-6 R5 medium tolerance 100 96 Neonectria ditissima Family Seeds sown Purpose Plant potted Cross

Pest and Disease screening 2016

• Phytophthora cactorum:

– Tests in seedlings following published method (verify pathogenicity of isolates) – 3 tray inoculated: segregation – 1 tray non inoculated: none dead!

Female Male M589 Evereste x C.G.30 200 Phytophthora cactorum Family Seeds sown Purpose Cross

Pest and Disease screening 2016

• Phytophthora cactorum:

– Tests in HWC (more inoculum than for seedlings)

– HWC randomized in root trainers – Inoculated 14/04/2016 – 15ml of inoculum – ~ 2 x 10-4 zoospores of P. cactorum – 2/3 isolate 418 & 1/3 of isolate 295

Pest and Disease screening 2016

• Phytophthora cactorum:

First observation 26/04 Fourth observation 13/05 Control Control M547-41 M546-110 M545-145 M482-54 M547-41 M546-110 M545-145 M482-54 M549-94 M482-153 M432-217 M482-17 M549-94 M482-153 M432-217 M482-17 M547-72 M482-158 M482-11 AR809-3 M547-72 M482-158 M482-11 AR809-3 AR10-3-9 R104 M432-250 M547-1 AR10-3-9 R104 M432-250 M547-1 M547-8 M482-65 M482-175 B24 M547-8 M482-65 M482-175 B24 REP 1 REP 1 M547-1 M546-110 M545-145 M482-11 M547-1 M546-110 M545-145 M482-11 B24 M549-94 M547-72 M432-217 B24 M549-94 M547-72 M432-217 M547-8 M482-13 M547-41 AR10-3-9 M547-8 M482-13 M547-41 AR10-3-9 R104 M482-175 M482-158 M482-54 R104 M482-175 M482-158 M482-54 M432-250 M482-65 AR809-3 M482-153 M432-250 M482-65 AR809-3 M482-153 REP 2 REP 2 M546-110 M482-158 R104 M547-41 M546-110 M482-158 R104 M547-41 B24 M482-65 M547-1 AR809-3 B24 M482-65 M547-1 AR809-3 M482-13 M432-250 M545-145 M547-8 M482-13 M432-250 M545-145 M547-8 AR10-3-9 M547-72 M482-175 M432-217 AR10-3-9 M547-72 M482-175 M432-217 M482-54 M482-11 M482-153 M549-94 M482-54 M482-11 M482-153 M549-94 REP 3 REP 3 M547-8 M545-145 M482-11 M547-1 M547-8 M545-145 M482-11 M547-1 AR10-3-9 M549-94 R104 M547-72 AR10-3-9 M549-94 R104 M547-72 M482-158 M432-250 M482-65 M546-110 M482-158 M432-250 M482-65 M546-110 M547-41 M482-153 M482-175 B24 M547-41 M482-153 M482-175 B24 M432-217 M482-13 M482-54 AR809-3 M432-217 M482-13 M482-54 AR809-3 REP 4 REP 4 AR10-3-9 M482-11 M547-8 M482-54 AR10-3-9 M482-11 M547-8 M482-54 M545-145 M547-72 M432-217 AR809-3 M545-145 M547-72 M432-217 AR809-3 M482-158 M432-250 M482-175 M546-110 M482-158 M432-250 M482-175 M546-110 M482-65 R104 M482-153 M547-1 M482-65 R104 M482-153 M547-1 M547-41 M549-94 B24 M482-13 M547-41 M549-94 B24 M482-13

Susceptible in every rep: M545-145 Never showed symptoms: M547-1 Higher replication needed Include controls SWC might be better and faster ~ seedlings

Pest and Disease screening 2016

• Fire blight (Erwinia amilovora):

– Tested at Wädenswil

14.03.2016 Graft units were moved to greenhouses 25.04.2016 plants were moved to security greenhouse GX; inoculation with E. amylovora 02.05.2016 disease progression measurement I 09.05.2016 disease progression measurement II 17.05.2016 disease progression measurement III 18.05.2016 experiment completed

Pest and Disease screening 2016

• Woolly apple aphid (Eriosoma lanigerum):

– Inoculation to verify previous years results

• ngoing

– Observation of overwintering colonies on root systems in pots made this year – All to be retested using young cuttings (faster results) – Deselection policy needed!!!

Material and methods

• 1,000 seeds of M597 (Evereste x G.C.202) were sown

into light compost on 4th of December 2015 and stored for 14 weeks at 2oC

• 7th of March moved to the glasshouse for germination at

Day Tempe > 18oC and Night Temp > 15oC

• Part of the seedlings germinated were selected for Fire

Blight resistance genes from Evereste (R_E) and from Robusta 5 (R_R5) through G.C.202

• WAA resistance is being evaluated through inoculation –

improve markers for R5 res (currently not close enough for pre-selection; 1 marker ~ 5 cM away)

Material and methods

Results

1 2 3 4 5 6 7 8 9 10 11 12 A Robusta5 M597_008 M597_016 M597_024 M597_032 M597_040 M597_048 M597_056 M597_064 M597_072 M597_080 M597_087 B M597_001 M597_009 M597_017 M597_025 M597_033 M597_041 M597_049 M597_057 M597_065 M597_073 M597_081 M597_088 C M597_002 M597_010 M597_018 M597_026 M597_034 M597_042 M597_050 M597_058 M597_066 M597_074 M597_082 M597_089 D M597_003 M597_011 M597_019 M597_027 M597_035 M597_043 M597_051 M597_059 M597_067 M597_075 M597_083 M597_090 E M597_004 M597_012 M597_020 M597_028 M597_036 M597_044 M597_052 M597_060 M597_068 M597_076 M597_084 M597_091 F M597_005 M597_013 M597_021 M597_029 M597_037 M597_045 M597_053 M597_061 M597_069 M597_077 M597_085 M597_092 G M597_006 M597_014 M597_022 M597_030 M597_038 M597_046 M597_054 M597_062 M597_070 M597_078 M597_086 M597_093 H M597_007 M597_015 M597_023 M597_031 M597_039 M597_047 M597_055 M597_063 M597_071 M597_079 Evereste CG202 M597 (Evereste x CG202)

Resistance linked to Robusta 5 and Evereste genes Resistance linked to Robusta 5 gene Resistance linked to Evereste gene Susceptible

Plates 1 to 4 – 372 seedlings

Retained: Total resistant seedlings 294 Discarded: 64 susceptible seedlings 18 failed or inconclusive

Sus 64 R_R5 78 R_E 124 Rx2 88

Seedling name Predicted Phenotype FEM14 FEM19 FEM47 CHFbE01 CHFbE02 CHFbE08 M597_001 R_R5 181 150 209 248 M597_002 Sus 248 M597_003 Rx2 182 150 209 248 231 248 M597_004 R_E 248 231 248 M597_005 Rx2 182 150 209 248 231 248 M597_007 Rx2 182 150 209 248 231 248 M597_009 R_R5 182 150 208 248 M597_010 R_E 247 231 248 M597_011 Sus 248 M597_013 R_E 248 231 248 M597_014 Rx2 182 150 209 248 231 248 M597_015 R_R5 182 150 209 248 M597_017 Rx2 181 150 208 247 231 248 M597_018 R_R5 181 150 209 248 M597_019 R_R5 181 150 209 248 M597_021 R_E 248 231 248 M597_022 Sus 248 M597_023 R_R5 182 150 209 248 M597_024 R_E 248 231 248 M597_026 Sus 248 MP_Robusta 5 MP_Evereste

Discuss set up for next pear trials

2013-2015 % rooted Quince PQ5-6 0 - 12 1 Quince PQ5-8 N/A Quince PQ5-13 0 - 25 Pear PQ34-1 6 - 35 50 Pear PQ34-3 8 - 12 25 Pear PQ34-6 5 - 52 2 Pear PQ35-2 0 - 14 16 Pear PQ35-3 0 - 59 6 Genus Selection Potted up 2016

• Material needed for

second stage trial

PQ37-2 10 PQ37-3 10 PQ37-5 6 PQ37-7 7 (2y old) PQ37-8 10 PQ38-2 6 PQ39-1 10 PQ39-3 12 PQ39-4 7 PQ39-5 5 # for grafting Selection

• Material for first

stage trial