Update for the Policy Group Meeting EM rootstock club Felicidad - - PowerPoint PPT Presentation

▶

Jan 20, 2024 284 likes •392 views

Update for the Policy Group Meeting EM rootstock club Felicidad Fernndez Fernndez Breeding update 2016 - 17 Seedling populations planted in 2016: Standard seedling populations: planted Jul 2016 Cross To be Seeds Family Female x Male

SLIDE 1

Update for the Policy Group Meeting EM rootstock club

Felicidad Fernández Fernández

SLIDE 2

Breeding update 2016 - 17

Seedling populations planted in 2016:

Seeds To be Female x Male Sown planted A469-4 x MH.12.3 M595 143 87 M.13 x Bud.9 M596 384 291 Evereste x AR295-6 M598 500 440 Novole x AR295-6 M599 495 120 Bud 9 x Evereste M600 504 460 M.116 x AR295-6 M601 255 218 M.13 x Geneva 11 M602 227 206

Total

1,822 Standard seedling populations: planted Jul 2016 Cross Family

Population pre-screened for FB and WAA: M597 (Evereste x C.G.202): 1,000 seedlings germinated 294 carrying FB-linked markers 74 resistant to WAA post-inoculation

SLIDE 3

Breeding update 2016 - 17

Crossing programme 2016:

Female X Male extracted stored aborted

Evereste

x Geneva 11

408 127 335 293 42 Geneva 30 AR440-1 434 49 160 89 71 Keep for seed store and repeat cross M.13

x AR295-6

297 101 526 469 57 M116 AR295-6 188 13 69 55 14 Keep for seed store and repeat cross Bud 9

X Evereste

276 184 786 736 50 Geneva 11

X AR295-6

53 5 16 10 6 Keep for seed store and repeat cross AR295-6

x Geneva 30

333 793 691 102 1,989 2,685 2,343

Female X Male

OHxF333

x Farmingdale

148 85 214 171 43

OHxF69

x BP2

117 27

Old Home

x BP3

162 48

Pyronia (x2)

x OHxF51

63 490 160 214 171 43

Cross

No. Fls
No. of fruit

set

No. of seeds

extracted

No. of

seeds stored Aborted Seeds Cross

No. Fls
No. Fruit set

# of seeds

SLIDE 4

Breeding update 2016 - 17

Seedling populations germinating in 2017:

♀ ♂ Year S M L AR86-1-20 x Geneva 11 2010 M603 200 4 M.9 x M.116 2013 M604 61 1 1 Novole x M.116 2014 M605 98 2 Evereste x Geneva 202 2015 M606 542 11 Evereste x Geneva 11 2016 M607 293 6 Evereste x AR295-6 2015 M608 976 20 M.13 x AR295-6 2015-16 M609 505 10 Bud 9 X Evereste 2016 M610 736 15 AR295-6 x Geneva 30 2015-16 M611 779 16 4,190 ♀ ♂ Year S M L BP1 x P. betulifolia 2010 PRP57 1150 23 OHF333 x Junsko Zlato 2010 PRP58 821 1 16 OHF51 x Pyronia (x2) 2015 PRP59 236 5 Old Home x BP3 2015 PRP60 227 1 4 OHF69 x BP2 2015 PRP61 69 1 1 OHF333 x Farmingdale 2016 PRP62 171 1 3 2,674 Trays Trays Cross Cross FAM Sown FAM Sown

SLIDE 5

Breeding update 2016 - 17

Seedling evaluation 2016:

Vigour, crop load, suckers and, in younger populations, fruit size were recorded in Sep-Oct 2016

2010: M553 (AR86-1-20 x Geneva 202) – 17 selections (2015&16) M554 (M.M.106 x Geneva 30) – 12 selections (2015&16) M555 (Geneva 30 o.p.) – 8 selections (2015&16) M556 (Ottawa 3 o.p.) 8 selections (2015&16) 2011: M557 (M.116 x M.9) M558 (Geneva 30 x M.116) M559 (Bud 9 x M.9) M560 (AR86-1-20 x Geneva 11) M561 (M.27 x Geneva 30) M562 (M.M.106 x Geneva 202) M563 (M.M.106 x Bud 9) 2012: M555a (Geneva 30 o.p.) M556a (Ottawa 3 o.p.) M559a (Bud 9 x M.9) M560a (AR86-1-20 x Geneva 11) M561a (M.27 x Geneva 30) M562a (M.M.106 x Geneva 202) M563a (M.M.106 x Bud 9) M564 (Geneva 202 x AR295-6) M565 (Bud 9 x M116) 2013: M566 (Bud 9 x Evereste) M567 (M.27 x Geneva 11) M568 (Torstein x M.27) M569 (Torstein x M.9) M570 (Geneva 202 op M571 (Geneva 11 op)

Additionally, suckers were recorded in in populations planted in 2014 (M573 – M586)

SLIDE 6

Breeding update 2016 - 17

Seedling evaluation 2016:

2011: Preliminary selections to be confirmed 2017 PRP45 (PB11-30 x OHxF87) PRP46 (B14 x op) PRP47 (BP1 x P. betulifolia) PRP48 (OHxF333 x Junsko Zlato) PRP49 (PB11-30 x OHxF333) PRP50 (OHxF87 x BP1) 2013: (recorded vigour and suckers only) PRP49a (PB11-30 x OHxF333) PRP50a (OHxF87 x BP1) PRP51 (OHxF87 x P525-3) PRP52 (BP1 x P525-3) some individual being evaluated as single-seedling stool beds

SLIDE 7

Marker Assisted Breeding

372 seedlings of M597 (Evereste x G.C.202) screened for Fire Blight

resistance genes from Evereste (R_E) and from Robusta 5 (R_R5) through G.C.202 in 2016

Resistance linked to Robusta 5 and Evereste genes Resistance linked to Robusta 5 gene Resistance linked to Evereste gene Susceptible

Phenotypic screening for Woolly Apple Aphid (WAA)

SLIDE 8

Marker Assisted Breeding

WAA resistance evaluated through inoculation

in September 2016. In total 235 seedlings were scored:

– 74 were found to be resistance – 161 developed colonies

SSR markers selection associated to resistance gene from Robusta 5

(Er2), two PCR multiplexes defined (WAA_MP_A and WAA_MP_B) –

n-going optimization and screening of M597

– Marker CH04f08 discarded for this family, same allele patter in both G.C.202 and Evereste

Marker Allele range Marker Allele range GD153 180-150 CH04c10 125-179 CH04c06 155-200 CH02g04 174-207 NH015a 98-169 SAmsCO963298 345-390 NZmsEE669355 175-200 NZmsMSAj1681 169-201 CH01h01 114-134 SAmsEE663640 476-485 SAmsCV150384 230-250 GD96 140-180 CH04f08 180-200 GD112 200-240 Hi01d06 95-165 NSmsEB145764 180-215 AT00174 180-210 WAA_MP_A WAA_MP_B

SLIDE 9

Pest and disease screening

Ongoing work: use multiplexes optimised for screening in M597 to

screen rootstock selections and cultivars for markers reportedly linked to resistance

Rootstock/cultivar Parentage Rootstock/cultivar Parentage AR10-2-5 M.27 x M.M.106 JM7

M. prunifolia Seishi x M9

AR10-3-9 M.27 x M.M.106

M. floribunda 821

unknown AR295-6 R.5 x Ottawa 3 M.13 unknown AR440-1 M.25 x M.27 M.7 unknown AR486-1 Ottawa 3 x M.7 Mildew Immune Sdlg unknown AR628-2 Ottawa 3 x M.M.106 Northern Spy unknown AR680-2 M.26 x M.27 Ottawa 3 M9 x Robin Crab AR682-6 M.26 x MI.793 R59 AR134-31 x AR86-1-23 AR801-11 M.26 x M.9 R80 AR134-31 x AR86-1-22 AR809-3 R.80 x M.26 Robusta 5 unknown AR835-11 MI.793 x M.9 SJM127

M. baccata 'Nertchinsk' x M.9

AR837-19 M.3 x M.1 SJM167

M. baccata 'Nertchinsk' x M.26

AR839-9 M.7 x M.27 SJM188

M. baccata 'Nertchinsk' x M.26

AR86-1-20 M.M.106 x M.27 SJP84-5217

M. robusta 5 x B. 57490

B24 AR10-2-5 x AR86-1-22 SJP84-5162

M. robusta 5 x M.27/M26*

R104 AR134-31 x AR86-1-22 SJP84-5174

M. robusta 5 x M.27/M26*

G.C.11 M.26 x R.5 SJM15

M. baccata 'Nertchinsk' x M.9

G.C.16 Ottawa 3 x M. floribunda 821 SJM189

M. baccata 'Nertchinsk' x M.26

C.G.30 R.5 x M.9 SJP84-5231

M. robusta 5 x M.27/M26*

G.C.41 M.27 x R.5 AR134-31 Robusta5J x MM106 G.C.202 M.27 x R.5 AR86-1-22 MM106 x M27 G.C.935 Ottawa 3 x R.5 AR86-1-23 MM106 x M27 M116 M.M.106 x M.27 Robin Crab unknown M25

N. Spy x M.2

M prunifolia Seishi unknown M26 M.16 x M.9 M16 unknown M27 M.13 x M.9 M2 unknown M20 Unknown M3 unknown M9 Unknown MI 793 Northern Spy x M.2 M1 Unknown AR10-2-6 M.27 x M.M.106 MM106 Northern Spy x M.1 Torstein unknown Pajam2 (M.9) Unknown A469-4 Howgate x (M platycarpa x M26) AR852-3 Unknown MH.10.1 unknown Bud 9 M8 x Red standard Novole Unknown Evereste Unknown