University of St Andrews iGEM Team 2012 1957 1983 2007 Modern - - PowerPoint PPT Presentation

University of St Andrews iGEM Team 2012

2007 1983 1957

All photos taken near Key West, Florida. Left to right: 1 & 2 Monroe County Library collection (Florida Keys) 3. Smithsonian Magazine (September 2008), Loren McClenachan

“Modern oceans have been so vastly altered by overexploitation of fishes as to be barely recognizable semblances of their pre- exploitation states.”

Callum Roberts 2007 The Unnatural History of the Sea

Fish depletion

Our model: “by 2100 only a fraction of today’s fish biomass will remain”

• Biodiversity loss
• Food problem
• Global omega-3 shortage

Model predictions for the future if we continue fishing at current rates

Modelling ω-3 fatty acid depletion ω-3 fatty acid synthesis

re source

Metal binding proteins Scientific impact

• f iGEM

Zoe Ashwood

Modelling fish population dynamics

Real-world fish biomass data

• 77 data points
• 234 sets of data extrapolated and combined
• 1932-2006 time series

Parameter tuning

Solid – model’s prediction of catch; Dots – real-world catch data Solid – model’s prediction of biomass; Dots – real-world biomass data

Model’s biomass prediction

Model's prediction of world fish biomass (solid), as it changes throughout the years between 1950 and 2100; as well as biomass data (dots).

Alternative aquaculture scheme

Omega-3 flow Wild: algae => fish => humans Aquaculture: algae => fish => fish oil => farmed fish => humans Fish meal is replaceable with non-fish products. Fish oil contains omega-3: wild fish input required (at present). Substitute with synthetic omega-3

Omega-3: required production levels

Green: fish-free aquaculture (current output of farmed fish). Purple: fish-free aquaculture (increased

• utput of farmed fish). Blue: world population provided with daily recommended intake of omega-3
• f 500mg per day

Model’s biomass prediction (restricted catch)

Model predictions for the future if we continue fishing at current rates (dashed) and if we reform our aquaculture habits (orange)

Modelling ω-3 fatty acid depletion ω-3 fatty acid synthesis

re source

Metal binding proteins Scientific impact

• f iGEM

Josi Buerger

Synthesising the ω-3 pathway

Figure modified from Livore V., Tripodi K., Utarro A., 2007. Elongation

• f polyunsaturated fatty acids in trypanosomatids. FEBS Journal, 274:

264–274.

18:1$(9)$ 18:2$(9,$12)$ 18:3$(9,$12,$15)$ 1$ 3 1$ 3$ 5$ 7$ 9$ 1$ 3 1$ 3$ 5$ 7$ 9$ 1$ 3 1$ 3$ 5$ 7$ 9$

Results

Fatty Acid Methyl Ester (FAME) analysis by Gas Chromatography Mass Spectrometry (GC-MS)

Lipid analysis

Modelling ω-3 fatty acid depletion ω-3 fatty acid synthesis

re source

Metal binding proteins Scientific impact

• f iGEM

Josi Buerger

New ¡Ni ¡binding ¡ sequence 6 ¡x ¡His GST

Short metal binding peptides

gBlocks

New ¡Ni ¡binding ¡ sequence 6 ¡x ¡His GST Precious)) metal) scavenger) Toxic) metal) scavenger)

1mbo

Modelling ω-3 fatty acid depletion ω-3 fatty acid synthesis

re source

Metal binding proteins Scientific impact

• f iGEM

Antti Korpimaki

Results summary

0" 20" 40" 60" 80" 100" 120" 140" 160" 180" 200"

2004" 2005" 2006" 2007" 2008" 2009" 2010" 2011"

Number'of' Year'

Teams" Parts"submi6ed"(10s)" Papers"men<oning"iGEM" Papers"men<oning"Parts"Registry" Papers"men<oning"specific"Registry"BioBricks"

iGEM – Registry relationship

Suggested citation style

Team, PartName, PartChassis, Date, [DOI or Link]

Conclusions

re source

• Successfully modelled past and future fish

biomass and our omega-3 requirement

• Built part of the omega-3 pathway in E. Coli
• Created novel metal binding peptide
• Quantified iGEM impact

Our sponsors

re source

Team St Andrews thanks those who made

• ur project possible

Dr Markus Gierth, Adam Boggon, Dr Morven Shearer, Professor Gordon Bell, Jesse H. Ausubel, Jason Fontana, Twitter's Bootstrap, DTU-Denmark 2011 (iGEM team), School of Geography & Geosciences, The Terry K. Smith Laboratory