Tracing radioactive molecular markers with pixel detectors - - PowerPoint PPT Presentation

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Tracing radioactive molecular markers with pixel detectors - - PowerPoint PPT Presentation

Aug 17, 2022 385 likes •774 views

INFN - Physics Department Universit Federico II Napoli, Italy M. Conti, E. Bertolucci, P. Russo Tracing radioactive molecular markers with pixel detectors

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INFN - Physics Department Università Federico II Napoli, Italy

  • M. Conti, E. Bertolucci, P. Russo

Tracing radioactive molecular markers with pixel detectors

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  • ♣

♣ ♣ ♣β

  • ♣

♣

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SLIDE 3
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  • 0.001

0.01 0.1 1 10 100 1000

resolution sensitivity dynamic range

film phosphor MWPC

(µP PLQLPXP DFWLYLW\

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SLIDE 5
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SLIDE 6
  • β
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SLIDE 7
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SLIDE 8
  • µ

µ

  • µ
  • 0HGLSL[&(51,1)18QLY)UHLEXUJ8QLY*ODVJRZ
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SLIDE 9

VME board PC-VME interface Mother board VME crate Chip board radioactive sample pixel detector electronics HV power supply LV power supply control logics, reference biases, test signal output

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SLIDE 10
  • )LJXUHFRXUWHV\RI06QRH\V
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SLIDE 11
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SLIDE 12
  • )LJXUHFRXUWHV\RI0&DPSEHOO
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SLIDE 13
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SLIDE 14
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SLIDE 15
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SLIDE 16
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SLIDE 17
  • β
  • µ

µ µ

  • SODVWLF

*D$V

≈µ ≈µ

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SLIDE 18
  • β
  • β
  • β
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SLIDE 19
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SLIDE 20
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SLIDE 21

β

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  • ±

±

50 100 150 200 250 5 10 15 20 25

35S / 14C experimental 35S / 14C simulation

Detector Counts (cps) Activity (Bq)

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  • ±

±

20 40 60 80 100 120 10 20 30 40 50

32P experimental 32P simulation

Detector Counts (cps) Activity (Bq)

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  • ≈

≈

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  • Probe-array
  • f

known gene sequences Unknown target sample is radio- labeled Hybridization probe-array / target Detector locates the marker Laboratory tool to develop clinical tests for : – multi-gene analysis (interaction between genes in disease development) – research

  • f

human gene mutations in patients – research

  • f

viral gene mutations

  • )LJXUHLVQRWDQH[SHULPHQWDOUHVXOW
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SLIDE 26
  • )LJXUHLVQRWDQH[SHULPHQWDOUHVXOW
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SLIDE 27
  • 0.0

0.5 1.0 0.0 0.5 1.0 BETAview (counts/cm

2)

MWPC (counts/cm

2)

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  • ≈
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  • 20

40 60 80 100 120 140 160 0.00 0.02 0.04 0.06 0.08 0.10

egg cell #1 egg cell #2

Count Rate (cps) Time (min)

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SLIDE 33
  • 20

40 60 80 100 120 140 0.0 0.5 1.0 Activity Ratio (ext/total) Time (min)

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SLIDE 34
  • µ
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SLIDE 35
  • ε

ε

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SLIDE 36
  • +9

&6 &6 &6 &6

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SLIDE 37