The Preservation and Cleanse of Lithic Cultural Relics with - - PowerPoint PPT Presentation

The Preservation and Cleanse

• f Lithic Cultural Relics with

Mineralization Method in Hangzhou

Hangzhou-H14Z Hangzhou-H14Z

Categories

• f lithic

relics in Hangzhou

Inscription Tower Statue

Surface of Feilai Peak Sculpture was Severely Ruined (2002)

Statue

Statue

Mountain Leaking near Ciyunling Sculpture (2002)

Contamination

by trains’ exhausts

White Tower

(2002)

Towers

Inscription

Old Method

Clothes Contrast Sculpture Contrast

Contamination

by trains’ exhausts

White Tower

(2002)

MEANWHILE WE NOTICED SOMETHING

BIZARRE

Why We Choose Calcium Oxalate

SEM of Calcium Oxalate 7 SEM of Calcium Carbonate7

Goal

CLEAN FORM SET

If we use chemical methods to generate calcium oxalate, we have to immerse the lithic relics in the chemical liquid; however, it is not suitable for the preservation of large-scale outdoor lithic relics. In fact, the natural existing calcium oxalate comes from the reaction of oxalic acid generated by microorganism and calcium compound on the surface of stone.

Old Ways of Oxalic Acid New Thoughts!

This research project will utilise genetic engineering methods to "graft" the gene that generates calcium oxalate into Escherichia coli (E. coil). Then oxalic acid reacts with calcium lithic relics to generate calcium oxalate, which stimulates its natural formation process. Finally, there forms a fine layer to protect the lithic relics.

According to Logistic Regression equation

• N: the number of

individuals in the group

• t: time
• N0: the original number of

individuals in the group

• r: the index of growth

potential of the group

• K: the maximum

environment capacity

using the data of r=0.550, K=1.937 and N0=0.1, we got the quantity of E.coli-time equation.

Modeling

Modeling

Assuming there is x% of E.colican be successfully expressed, we used the equation in last slide to multiplyby the x% to get the quantity of expressed bacteria-time equation,fex .

Here is a graph to help to visualize the equation.

Modeling

Assume a unit of expressed bacteria can have OxalicAcid produce speed of

• v. we can get total speed equationbymultiplyingv to last equation.

By integratingthe total speed equation, we can get the quantityofOxalic Acid-time equation,Soa . Here is a graph to help to visualize the equation.

Modeling

Because the decomposition speed should be direct proportion to quantity of remain lipid and quantity of expressed bacteria, we got the differential equation: dn/dt=k*(N-n)*fex By solving this equation, we found the relationship between remain lipid and time, which is Slp above.

n: decomposed lipid N: initial quantityof lipid k: a constant related to efficiency t: time

Modeling

Escherichia coli

Parts

LacI R0010 B0034 OAH B0015

E X S P N S

EcoRI XbaI SacII NheI SpeI PstI LacI R0010 B0034 Lipase B0015

E X S P N S

EcoRI XbaI SacII NheI SpeI PstI Bba_K1671001 Bba_K1671000

LacI R0010 B0034 OAH B0015

E X S P N S

EcoRI XbaI SacII NheI SpeI PstI

Bba_K1671000 Oxaloacetate Acetylhydrolase

Oxaloacetic Acid Oxalic acid

LacI R0010 B0034 Lipase B0015

E X S P N S

EcoRI XbaI SacII NheI SpeI PstI Bba_K1671001

Triacylglycerol Acylhydrolase

Oil

Lipase PCR Primer

• E. coli

Sequencing Cutting Test

Glyceryl Tributyrate Glyceryl Tributyrate + Victoria Blue Victoria Blue Lipase Lipase

FUTURE

Human Practice Human Practice

What did we focus on?

lCollaboration lPublic Outreach & Ethics lEnd-User Consideration lEducation

Collaboration

Zhejiang University Team Hangzhou #4 High School Team

Public Outreach & Ethics

• “Transgenosis Food”
• Questionnaires & Postcards
• Twitter

Transgenosis Food

Questionnaire & Postcards

88% 11% 1% 12%

HOW DO YOU KNOW ABOUT LITHIC RELICS?

Do not Know at All Do not know the preservation of Lithic Relics Know the protection of Lithic Relics

Twitter

End-User Consideration

“According to the principle, there is certain feasibility, because the principle

• f chemical coating is the main

component of waterproof material, such as sodium silicate, can quickly and effectively concrete structure layer of calcium hydroxide, calcium, calcium silicate, such as reaction, to form the wool stoma of inert crystals embedded in concrete, to airtight micro cracks, thereby increasing the compactness of concrete surface layer and the compressive strength. In the study of the biological mechanism of action of anticorrosion materials and similar is feasible in theory, but in the specific application, also need to further study

• f the role of timeliness, economy.”
• ----- Prof. Huang

Civil Engineering

Education

Presentation School’s Official Website

1.Li Haijun, Wang Lingang, Wang Zhize, Chen Fang, and Gao Jianfeng Induced Secretion Expression of Lipase Gene from Bacillus subtili in E. coli 2.Chen Chen, Qihong Sun, Buvaneswari Narayanan, Donald L. Nuss, and Osnat Herzberg Structure of Oxalacetate Acelhydrolase, a Virulence Factor of the Chestnut Blight Fungus

• 3. Nanjun Li, Hangzhou No. 14 High School

Study Hangzhou Relics Preservation from Feilai Peak-- Hangzhou Lithic Relics General Research

• 4. Shiyi Li, Kexie Zhang, Liya Wang

Hangzhou Feilai Peak Lithic Relics Preservation

• 5. Bingjian Zhang, Haiyan Yin, Zhongyue Shen, Huanming Lu

The Forming Mechanism and Chemical Bionics of Crude Calcium Oxalate Bio- mineralised Film---- Development of a New Surface Conservation Material for Historic Stone Relics

• 6. Xiumin Yu, Xiuying Tong, Xiuling Yu

The Application of Preserving Stone Relics through Bacteria

• 7. Xinping Li, Shandong University

Crystallization Behavior of Inorganic Minerals in Calcium Alginate Gels

Bibliography

Acknowledgment and appreciation

• Prof. Jianhong Luo, from Zhejiang University

Dr.Yingang Yan from Zhejiang University Hangzhou #14 High School Bio-technology Laboratory Sponsor Hangzhou #14 High School (Special Found for experiment reagent and apparatus) HangzhouMutisecience Lianke Biotech Company

Thanks for listening!