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Snail Model System Intro to snails as a model system in - PowerPoint PPT Presentation

Mar 31, 2023 •279 likes •577 views

Snail Model System Intro to snails as a model system in neurobiology Our lab snail Pond snail brains Identified neurons in pond snail brains dorsal ventral Snail Buccal Ganglia Buccal ganglia neurons Smail Week 1. Firing

  1. Snail Model System �

  2. Intro to snails as a model system in neurobiology � Our lab snail �

  5. Pond snail brains �

  6. Identified neurons in pond snail brains � dorsal ventral

  7. Snail Buccal Ganglia

  8. Buccal ganglia neurons �

  9. Smail Week 1. Firing properties of snail neurons �

  10. Responses to current injection �

  11. Firing Accomondation �

  12. Post-inhibitory rebound �

  13. Burster neurons �

  14. Snail week 2. Ionic mechanisms of excitability � Reduced Na � Reduced Ca �

  15. K+ Channel block with TEA �

  16. K+ Channel block with TEA/Cs �

  17. TEA/CS block progressing over time (5 minutes) �

  18. K+ Channel block with barium �

  19. Excitability mechanisms: Voltage Clamp � Describing the ionic conductances underlying the AP �

  20. Voltage clamp technique to examine ionic currents �

  21. Current across membrane � I m = I Rm + I Cm � Problems analyzing conductances underlying AP � � 1. I Cm obscured I Rm � 2. Conductances changed during AP � �

  22. Ohm’s law for V-Clamp �

  23. Ionic driving forces at rest �

  24. Ionic driving forces at rest �

  25. Current across membrane with hyperpolarizations � I m = I Rm + I Cm �

  26. Current across membrane with weak depolarizations �

  27. Current across membrane with strong depolarizations �

  28. I K � Measure I � I Na �

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