S Background Data from Thyssen et al., The epidemiology of contact - - PowerPoint PPT Presentation

▶
s background
SMART_READER_LITE
LIVE PREVIEW

S Background Data from Thyssen et al., The epidemiology of contact - - PowerPoint PPT Presentation

Nov 12, 2022 477 likes •784 views

S Background Data from Thyssen et al., The epidemiology of contact allergy in the general population prevalence and main findings, Contact Dermatitis 2007: 57: 287299. S The proportion of nickel contact allergy prevalence out of

slide-1
SLIDE 1

S

slide-2
SLIDE 2
slide-3
SLIDE 3

S The proportion of nickel contact allergy prevalence out of the proportion of

contact allergy prevalence to at least 1 allergen derived from published studies between 1966 and 2007

Background

Data from Thyssen et al., The epidemiology of contact allergy in the general population – prevalence and main findings, Contact Dermatitis 2007: 57: 287–299.

slide-4
SLIDE 4

Systemic allergy syndrome Allergic contact dermatitis Classified as carcinogenic

Background

Nickel-related pathologies

slide-5
SLIDE 5
slide-6
SLIDE 6

A little Hel…p(ylori) from my friend

slide-7
SLIDE 7

Sensing

slide-8
SLIDE 8
slide-9
SLIDE 9

HpNikR

Data from Dian et al., Structural Basis of the Nickel Response in Helicobacter pylori: Crystal Structures of HpNikR in Apo and Nickel-bound States. J Mol Biol. 2006; 25;361(4):715-30.

The ¡protein ¡

slide-10
SLIDE 10

HpNikR

S ICP-AES assay results

Metal-­‑binding ¡activity ¡

slide-11
SLIDE 11

HpNikR

S ATR-FTIR spectroscopy

assay results Conformational ¡changes ¡

slide-12
SLIDE 12

S Raman spectroscopy assay results

HpNikR

Conformational ¡changes ¡

slide-13
SLIDE 13

HpNikR

S CryoTEM microscopy results

slide-14
SLIDE 14

Promoters

intFor pnikRev pexbFor pnikFor pexbRev

PCR results

PCR scheme to i s o l a t e t h e promoters

slide-15
SLIDE 15

HpNikR

S Characterization of its

binding to pnikR promoter

DNA-­‑binding ¡activity ¡

slide-16
SLIDE 16

HpNikR

S Characterization of its

binding to double divergent promoter pnikR-pexbB

DNA-­‑binding ¡activity ¡

slide-17
SLIDE 17

Quorum sensing and storage

slide-18
SLIDE 18

Storage

S Hpn aminoacid sequence: Histidine residues are highlited

HH H HHHHHHH HH HH H HHHHHH HH HH H HH

Hpn ¡protein ¡

slide-19
SLIDE 19

Quorum sensing

slide-20
SLIDE 20

Applications

S Purification plants implementation

  • f heavy-metal removing bacteria
slide-21
SLIDE 21

Applications

S Probiotics to sequester the nickel

excess in the intestine

slide-22
SLIDE 22

Human practices

S Team presentation seminar

slide-23
SLIDE 23

S We are the founders (together

with other researchers) of BioBANG, Biotechnologies Association for the Next Generation

Human practices

slide-24
SLIDE 24

S The wet lab part of our Team

t o o k p a r t t o Playdecide (www.playdecide.eu) to discuss about Orphan Drugs and Stem Cells, an event organized for the European Biotech Week.

Human practices

slide-25
SLIDE 25

Human practices

S To analyze how much synthetic

biology, its applications and its ethical implications are known in

  • ur academic world, we decided

to submit a short survey. We submitted it to professors, researchers, doctoral students and non graduate students.

The ¡survey ¡

slide-26
SLIDE 26

Human practices

S

B) Given the possibility to engineer an organism to perform a given function in a controlled way, which perspectives can be profiled, in your opinion?

  • 1. I have great expectations for applications in

Human health.

  • 2. I think that this kind of research will never come
  • ut of laboratories.
  • 3. I think it may profile scenarios of genetic

pollution.

  • 4. I have no idea.

S

A) Did you know the term Synthetic Biology (as an evolution of the concept of Genetic Engineering)?

  • 1. I know it and i know about its applications.
  • 2. I have some ideas, but not so clear.
  • 3. I never heard about it.

RESULTS

The ¡survey ¡

slide-27
SLIDE 27

Human practices

S D) In which field you think that Synthetic

Biology may have better applications?

  • 1. Environmental
  • 2. Biomedical
  • 3. Food and Agricultural
  • 4. Energetic

S C) “Everything you can imagine, Nature has already created”, quoting A. Einstein. You think

that:

  • 1. Researchers are arrogant when they think of improving nature.
  • 2. It is important that science could take advantage of theoretical knowledge for applicative

uses.

  • 3. The efforts of research could never overcome the efficiency of natural systems.
  • 4. I have no interest in these implications.

RESULTS

The ¡survey ¡

slide-28
SLIDE 28

Achievements

S Designed 38 parts (from K1151000 to K1151038). S Submitted 15 parts to the Registry (K1151001, K1151005,

K1151006, K1151008, K1151009, K1151010, K1151012, K1151017, K1151018, K1151019, K1151024, K1151026, K1151034, K1151036).

S 10 parts we designed are working as we expected (K1151000,

K1151002, K1151003, K1151004, K1151006, K1151009, K1151010, K1151011, K1151036, K1151038).

S Outreached to the general public with public events and public

interventions.

slide-29
SLIDE 29

S