S Background Data from Thyssen et al., The epidemiology of contact - PowerPoint PPT Presentation

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Background Data from Thyssen et al., The epidemiology of contact allergy in the general population – prevalence and main findings, Contact Dermatitis 2007: 57: 287–299. S The proportion of nickel contact allergy prevalence out of the proportion of contact allergy prevalence to at least 1 allergen derived from published studies between 1966 and 2007

Background Nickel-related pathologies Systemic allergy syndrome Allergic contact dermatitis Classified as carcinogenic

A little Hel…p(ylori) from my friend

Sensing

HpNikR The ¡protein ¡ Data from Dian et al., Structural Basis of the Nickel Response in Helicobacter pylori: Crystal Structures of HpNikR in Apo and Nickel-bound States. J Mol Biol. 2006; 25;361(4):715-30.

HpNikR Metal-­‑binding ¡activity ¡ S ICP-AES assay results

HpNikR Conformational ¡changes ¡ S ATR-FTIR spectroscopy assay results

HpNikR Conformational ¡changes ¡ S Raman spectroscopy assay results

HpNikR S CryoTEM microscopy results

Promoters intFor pexbFor pnikRev PCR scheme to i s o l a t e t h e promoters pnikFor pexbRev PCR results

HpNikR DNA-­‑binding ¡activity ¡ S Characterization of its binding to pnikR promoter

HpNikR DNA-­‑binding ¡activity ¡ S Characterization of its binding to double divergent promoter pnikR-pexbB

Quorum sensing and storage

Storage Hpn ¡protein ¡ S Hpn aminoacid sequence: Histidine residues are highlited HH H HHHHHHH HH HH H HHHHHH HH HH H HH

Quorum sensing

Applications S Purification plants implementation of heavy-metal removing bacteria

Applications S Probiotics to sequester the nickel excess in the intestine

Human practices S Team presentation seminar

Human practices S We are the founders (together with other researchers) of BioBANG, Biotechnologies Association for the Next Generation

Human practices S The wet lab part of our Team t o o k p a r t t o Playdecide (www.playdecide.eu) to discuss about Orphan Drugs and Stem Cells, an event organized for the European Biotech Week.

Human practices The ¡survey ¡ S To analyze how much synthetic biology, its applications and its ethical implications are known in our academic world, we decided to submit a short survey. We submitted it to professors, researchers, doctoral students and non graduate students.

Human practices The ¡survey ¡ S A) Did you know the term Synthetic Biology (as an evolution of the concept of Genetic Engineering)? 1. I know it and i know about its applications. 2. I have some ideas, but not so clear. RESULTS 3. I never heard about it. S B) Given the possibility to engineer an organism to perform a given function in a controlled way, which perspectives can be profiled, in your opinion? 1. I have great expectations for applications in Human health. 2. I think that this kind of research will never come out of laboratories. 3. I think it may profile scenarios of genetic pollution. 4. I have no idea.

Human practices The ¡survey ¡ S C) “Everything you can imagine, Nature has already created” , quoting A. Einstein. You think that: 1. Researchers are arrogant when they think of improving nature. 2. It is important that science could take advantage of theoretical knowledge for applicative uses. 3. The efforts of research could never overcome the efficiency of natural systems. 4. I have no interest in these implications. RESULTS S D) In which field you think that Synthetic Biology may have better applications? 1. Environmental 2. Biomedical 3. Food and Agricultural 4. Energetic

Achievements S Designed 38 parts (from K1151000 to K1151038). S Submitted 15 parts to the Registry (K1151001, K1151005, K1151006, K1151008, K1151009, K1151010, K1151012, K1151017, K1151018, K1151019, K1151024, K1151026, K1151034, K1151036). S 10 parts we designed are working as we expected (K1151000, K1151002, K1151003, K1151004, K1151006, K1151009, K1151010, K1151011, K1151036, K1151038). S Outreached to the general public with public events and public interventions.

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