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The American Bullfrog (Lithobates catesbeianus)
Background
An American Bullfrog in Brazil Lauren V. Ash University of Vermont - - PowerPoint PPT Presentation
An American Bullfrog in Brazil Lauren V. Ash University of Vermont - - PowerPoint PPT Presentation
An American Bullfrog in Brazil Lauren V. Ash University of Vermont June 4, 2019 Background The American Bullfrog ( Lithobates catesbeianus ) Background Hoverman et al. 2011 Background High viral loads? Background Are invasive Bullfrogs in
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Background
Hoverman et al. 2011
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Background
High viral loads?
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Are invasive Bullfrogs in Brazil harboring the same levels of ranavirus?
Hypothesis: Ranaviruses have spread beyond the Bullfrog facilities We predict Bullfrogs will harbor the virus in high levels, similar to their native counterparts
Background
- Dr. Karla
Campião, UFPR
- Dr. Domingos
Rodrigues, UFMT
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An extra layer: helminths
Not much is understood regarding helminth parasite dynamics and diversity
Background
Nematodes/ro undworms Platyhelminthes/flatwor ms Ancanthocephalins/thorn y headed worms
Caused by trematode (Ribeiroia sp.)
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Is there an association between ranavirus and helminth parasites?
Background
vs
1 2 3 4 5 6 Yes No 5 10 15 20 25 30 35 Yes No 5 10 15 20 25 30 35 Yes No
No difference
More parasites in Ranavirus positive More parasites in Ranavirus negative
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Brazil Maps
Background Methods
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Background Methods
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Methods
Background Methods
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Background Methods
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Background Methods Results
8/9 ~89% 1/9 ~11% 3/3 ~100%
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Site Prevalence
Background Methods Results Background Methods Results
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Viral copies per uL
Background Methods Results Background Methods Results
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Parasite Abundance
Background Methods Results
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Parasite Abundance and viral load
Background Methods Results
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Summary
- The first comprehensive survey and
- ne of the first records of ranavirus
in natural amphibian populations in Brazil
- Detected low level infections in
Brazilian adult Bullfrog populations
- Individuals and sites with ranavirus
presence seem to have decreased numbers of parasites but does not correlate to the amount of virus
Background Methods Results Conclusions
vs
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Next Steps
- Are the sites that are
ranavirus positive close to active Bullfrog farms?
- Sequencing to determine
which ranavirus species
- Is this impacting native
anurans in Brazil?
Background Methods Results Conclusions
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ACKNOWLEDGEMENTS
John Wheeler Award Graduate College Summer Fellowship The Ronald Suiter Prize
- Dr. Roberto Fabri Fialho Award
Funding
The Gotelli lab Landowners UVM Biology faculty and graduate students All of my undergraduate research assistants
Every volunteer!
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Questions?
@lvash23
Email: lvash@uvm.edu