progeny psyllids healthy plants ~30-60% PCR+ ~30-60% PCR+ Healthy - - PowerPoint PPT Presentation

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Jun 03, 2023 162 likes •300 views

0 days 30 days Adult psyllids put Remove adult into cage with progeny psyllids healthy plants ~30-60% PCR+ ~30-60% PCR+ Healthy citrus 15 days Remove all input psyllids Bob Shatters and Chuck Powell (Fort Pierce), Nabil Killiny (Lake

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0 days Adult psyllids put into cage with healthy plants ~30-60% PCR+ 30 days Remove adult progeny psyllids ~30-60% PCR+ 15 days Remove all input psyllids Healthy citrus

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Bob Shatters and Chuck Powell (Fort Pierce), Nabil Killiny (Lake Alfred, and Bryce Falk (UC Davis)

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CP p18 p20 p13 p23 CPm RdRp L1 L2

HSP70h p61 p6 p33

HEL IDR

Anti-Clas gene

Target sequence for psyllid gene inserted into CTV

CTV vector for RNAi

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siRNA siRNA siRNA siRNA siRNA siRNA siRNA siRNA siRNA siRNA siRNA

siRNA

plant sieve element Peptides would be taken up the same as siRNAs Psyllid ?? CTV-infected cells

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5 10 15 20 25 30 BS4-2 DF-2 HSP70-3 JHAMAT JHE-3 PC1-1 Osiris Control Healthy Day 0 Day 1 Day 3 Day 5 Day 6 Day 7 Day 8 Day 10 Day 13 Day 14 Day 15

SUMMARY OF SURVIVAL OF ADULT PSYLLIDS ON RNAi PLANTS

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0 days Adult psyllids put into cage with healthy plants ~30-60% PCR+ 30 days Remove adult progeny psyllids ~30-60% PCR+ 15 days Remove all input psyllids Healthy citrus

CTV vector for RNAi

plant sieve element Peptides would be taken up the same as siRNAs Psyllid ?? CTV-infected cells

SUMMARY OF RNAi CAGE EXPERIMENTS – PSYLLID PROGENY