progeny psyllids healthy plants ~30-60% PCR+ ~30-60% PCR+ Healthy - PowerPoint PPT Presentation

Jun 03, 2023 •162 likes •294 views

0 days 30 days Adult psyllids put Remove adult into cage with progeny psyllids healthy plants ~30-60% PCR+ ~30-60% PCR+ Healthy citrus 15 days Remove all input psyllids Bob Shatters and Chuck Powell (Fort Pierce), Nabil Killiny (Lake

0 days 30 days Adult psyllids put Remove adult into cage with progeny psyllids healthy plants ~30-60% PCR+ ~30-60% PCR+ Healthy citrus 15 days Remove all input psyllids
Bob Shatters and Chuck Powell (Fort Pierce), Nabil Killiny (Lake Alfred, and Bryce Falk (UC Davis)
CTV vector for RNAi Target sequence for psyllid gene inserted into CTV Anti-Clas gene p18 p20 p33 HSP70h L1 L2 MT HEL IDR p13 p23 RdRp p6 p61 CP CPm
Psyllid ?? plant CTV-infected cells siRNA siRNA siRNA siRNA siRNA sieve siRNA siRNA siRNA siRNA element siRNA siRNA siRNA Peptides would be taken up the same as siRNAs
SUMMARY OF SURVIVAL OF ADULT PSYLLIDS ON RNAi PLANTS 30 25 Day 0 Day 1 Day 3 20 Day 5 Day 6 15 Day 7 Day 8 10 Day 10 Day 13 Day 14 5 Day 15 0 BS4-2 DF-2 HSP70-3 JHAMAT JHE-3 PC1-1 Osiris Control Healthy
SUMMARY OF RNAi CAGE EXPERIMENTS – PSYLLID PROGENY

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