PONDWEEDS MARIA KUZMINA UNIVERSITY OF GUELPH, CANADA PONDWEEDS WITH - PowerPoint PPT Presentation

PONDWEEDS MARIA KUZMINA UNIVERSITY OF GUELPH, CANADA

PONDWEEDS WITH US – FORESTS IN THE WATER, UNDER AND ABOVE Linear-leaved Broad-leaved Diversifolius

WHY MONITORING THEM AND WHAT MAKES IT DIFFICULT?  Ecological studies : food and shelter for many species of organisms  Bioindication : species-specialists and generalists towards the alkalinity and pollution  Protection : 6 species at risk or special concern in North America, among those 2 from Ontario (Hill’s and Ogden’s pondweeds)  A quatic habitats less accessible compared to terrestrial ones  M orphologic identification is limited by phenology and microscopic characters

WHAT ARE THEY AND HOW MANY? ALISMATALES POTAMOGETONACEAE Potamogeton (32-37 species) Stuckenia (4 species) Zannichellia (1 species) 30 species in Ontario

BACKGROUND AND OBJECTIVES eDNA detection of plants BROAD SPECIFIC • Invasive aquatic species Detection of the species of (Scriver et al., 2015) pondweeds (Potamogetonaceae) in water samples Requested and funded by the • Large-scale monitoring in soil Species at Risk (Fahner et al., 2016) Research Fund of Ontario (SARRFO, 2016- 2017,10K)

PREPARING LOCAL REFERENCE LIBRARY 30 species of pondweeds reported from Ontario (VASCAN) 90 specimens : collection of DNA from CBG, part of “The Vascular Plants of Canada” project ( dx.doi.org/10.5883/DS-POTAM) * * Potamogeton foliosus (Agriculture and Agrifood Herbarium, DAO) identified by Barre Hellquist* , the author of the treatment of pondweeds in the “Flora of North America”

SELECTION OF THE MARKERS & DESIGNING THE PRIMERS • atpB - rbcL (Ito et al., 2014) • ITS2 (China Plant BOL Group, 2011) Datasets downloaded from GenBank ( atpB - rbcL ) & BOLD (ITS2) Designing primers specific for g Potamogetonaceae Datasets of atpB-rbcL & ITS2 generated for the local library g Designing eDNA primers eDNA marker Length (with mean length) (bp) 101 ‒ 133 atpB-rbcL-117 161 ‒ 208 atpB-rbcL-184 133 ‒ 182 ITS2-157