Metabolomic analysis revealed that green tea polyphenols decreased - - PowerPoint PPT Presentation

Metabolomic analysis revealed that green tea polyphenols decreased the formation of microbial metabolites of aromatic amino acids in humans

Yuyin Zhou1, Ningning Zhang1, Andrea Arikawa2, and Chi Chen1,*

1 Department of Food Science and Nutrition, University of Minnesota

1334 Eckles Ave, St Paul, MN 55108-1038

2 Department of Nutrition & Dietetics, University of North Florida

1 UNF Drive, Jacksonville, FL 32224-7699

* Corresponding author: chichen@umn.edu

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Minn nnes esota Gr Green een T Tea Trial ( (MGTT)

• Subjects: 120 postmenopausal women aged 50-70 years, with high mammographic

density (>50% fibroglandular tissue).

• Treatment: subjects taking green tea capsules (800 mg epigallocatechin gallate

(EGCg)/day) or placebo for one year.

• Sample group:
• --P0: placebo before trial
• --P12: placebo after trial
• --T0: GTPs treatment before the trial
• --T12: GTPs treatment after the trial
• Urine and fecal samples were collected and analyzed by LC-MS-based metabolomics.

https://clinicaltrials.gov/ct2/show/NCT00917735 LC-MS MDA

P0 P12 T0 T12

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Modeling of fecal and urine samples Urine s samples Fecal s samples

P0 P12 T0 T12

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Identification of GTP-responsive markers

Indole-3-carboxaldehyde Phenyl-acetylglutamine Hippuric acid Indoxyl sulfate

Urine s samples Fecal s samples

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Identification of GTP microbial metabolite markers

• A group of phenolic acids derived from microbial metabolism
• f GTPs were identified as the robust exposure markers.
• Major GTPs were not found in significant amounts in feces.

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Identification of GTP-responsive markers

• Chronic GTPs treatment decreased the levels of microbial

metabolites of aromatic amino acids in urine and feces.

• However, GTP treatment did not significantly affect the levels of

short chain fatty acids and secondary bile acids, two other major groups of microbial metabolites.

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Microbiome analysis (16S)

• Chronic GTP

did not change the microbial composition.

0% 20% 40% 60% 80% 100% T0 T12 NA Verrucomicrobiae Thermoplasmata Synergistia Saccharimonadia Oxyphotobacteria Negativicutes Mollicutes Methanobacteria Melainabacteria Lentisphaeria Gammaproteobacteria

OTU numbers Alpha diversity

PCoA

T0 T12 T0 T12

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GTP

O HO OH O OH OH OH O OH OH OH

Tyrosine Phenylalanine Tryptophan

N H COOH OH

COOH

COOH OH HO COOH HO HOOC OH OH HOOC OH OH OH HOOC OH OH COOH HO

HO COOH COOH COOH OH COOH

Phenylacetyl- glutamine

N H COOH

N H CHO

Dehydroxylation Decarboxylation Decarboxylation Dehydroxylation Dehydroxylation Hippuric acid Decarboxylation Indole-3- carboxaldehyde Indoxyl sulfate

Aromatic amino acids Potential competitive inhibitions between microbial metabolism of GTPs and aromatic amino acids

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Acknowledgement Any questions, please email me.

zhou0882@umn.edu This work was partially supported by the NIFA project No. MIN-18-092 . The human samples were from a NIH project 5R01CA127236-06. We thanks Dr. Hamed Samavat and Ms. Sarah Bedell for their assistance. The authors declare no conflict of interests.

Conflict of interests