Mechanical Bioreactor Integrated Systems Amanda Castagnino Emily - - PowerPoint PPT Presentation

Mechanical Bioreactor Integrated Systems

Amanda Castagnino Emily Adams Emily Wood Natalie Nold Simran Singh

Agenda

PHASE III GOALS

1. Achieve specific device movement. 2. Machine parts for device mount. 3. Complete static cell culture testing in PDMS chamber. 4. Complete material testing on heat-cured PDMS.

PHASE III DELIVERABLES

1. Machined device pieces 2. Cell Culture Images 3. Updated Risk Assessment 4. Updated Bill of Materials 5. MSD II Project Plan

New Membrane Holder: Solving Sterility Issue

Update on Machining

Timeline

• Submitted Parts 3/9
• Miscommunication
• Miscommunication

resolved 3/19

• 2 parts received by

3/27

• Reached out to Mike,

Debartolo, and Rick

• WIll have new time

estimate 3/30

Machining Problem Tracking...

Rating Description Identifying & Selecting Problem PSP 1 Analyzing Problem PSP 2 Generating Potential Solutions PSP 3 Selecting & Planning Solution PSP 4 Implementing Solution PSP 5 Evaluating Solution PSP 6 R1 R2 R3 Y4 Y5 G6 Major Machining parts takes much longer than expected 5 hours put into machining with no parts completely finished Not enough trained team members and experience to make designed parts Ask machine shop to machine parts for us Consult machine shop and get time estimate Major Time 10 days since files submitted, only 2 parts done unclear communication with machine shop

• pen up

communication with shop and reach out to debartolo Connected with debartolo and Houdini meeting with machine shop Friday Incorrect tapping tolerances Provided incorrect tapping tolerances to machine shop Lack of machining knowledge determine correct size of tapped holes edit and resubmit files files resubmitted Sheet metal drawing have not completed draw for membrane holder do no know how to create sheet metal drawing delegate task to amanda, who will learn how complete drawing Pegged Plate Pegs are not perpendicular Pegs Tapped poorly Confront shop Remake part Arm Complicated piece to machine needs welding and press-fit Consult Houdini Houdini is making part Major Mold Complicated piece to machine needs press-fit and high tolerances Consult Houdini

Device Movement Update

Actuator Precision

% Strain Tolerances

% Strain Stretch (mm) % Range w/ accuracy (using 1.4%) 5 2 3.6 - 6.4 10 4 8.6 - 11.4 15 6 13.6 - 16.4 20 8 18.6 - 21.4 Varying thickness in PDMS chamber could lead to a greater proportion of stretching in the well

• f the chamber.

Material Testing with Heat-Cured PDMS

PDMS cured at 100°C for 35 minutes. Testing tab slipping reduced with heat-cured samples. “N” Control “I” Incubated at 37°C three days before material testing

Results

Results

Results

Sylgard 184 TDS:

Tensile Strength (6.7MPa)

Strains:

Standard (58.8%) - 43.9% Worst Case Incubator (55.5%) - 33.1% Worst Case

Static Cell Culture Images

10X Objective - Leica 4X Objective - Leica 2.5X Objective - Leica

Risk Assessment

Limited project time increases risk

Bill of Materials

Machining and cell culture supplies purchased Total Budget Left ~ $350

MSD II Project Plan - Final Phase

Finish machining parts and creating cycling programs Test experiment conditions Complete user documentation Test with subjects as much as possible

Project Deadlines

Finished final poster Technical paper submitted for review Finished EDGE website Performance vs Requirements Lightning Talk Slide Technical Paper due Industrial Advisory Board Demo Imagine RIT Festival Workspace clean Final Gate Review April 19 April 24 April 26 April 27 April 28 May 2 May 2-4

Three-Week Plans

Amanda Emily A. Emily W. Natalie Simran

Dynamic cell culture testing in PDMS chamber Device 3-D printing and machining Finish machining device parts Finalize programs for device Verify % strain movement and tolerances Finalize technical report Create Imagine RIT poster Finalize device documentation Dynamic cell culture testing in PDMS chamber Create guide for cell imaging and analysis