Maker Assisted Selection on Varietal Improvement Program of Salt - - PowerPoint PPT Presentation

Maker Assisted Selection on Varietal Improvement Program of Salt Tolerant Rice in Myanmar

Tin Tin Myint & Thida DAR, Myanmar

WORKSHOP TITLE

Quick Conversion of Popular Rice Varieties with Emphasis on Drought, Salinity and Grain quality improvement RGDU, GCP & BIOTEC

Duration: 1-15 May,2008 (3rd Training) Place : RGDU, Kasetsart University, Kamphaengsean, Nakorn Pathom, Thailand

Objectives

• To develop cultivar with enhanced

tolerance to prevailing salt stress

• To apply the Molecular Marker Assisted

Selection on advanced breeding program

Materials

• Pokkali x IR53936 No.of Families
• BC4F2

170 leaf samples 8

• BC3F5 412 leaf samples 42
• Total 582 leaf samples 50

Methods

• Marker Assisted Selection
• Agarose Gel Electrophoresis
• Poly Acrylamide Gel Electrophoresis
• Used Markers- SalT, RM 10720, RM 3412

RM 1287, RM 411, RM10772 Waxy, RM5349, RM457, RM21

P2 Pokkalia(salt) R/F&BIOTEC MAS

Year ×

P1 IR53936( HYV)

F1 ×

IR53936 BC2F1 (4plts)

×

IR53936 BC1F1 (7plts)

×

IR53936 BC3F1(22plts) BC3F2(245plts) BC3F3(245lines) MAS MAS DAR 2003DS 2003WS 2004DS 2004WS 2005DS 2005WS 2006WS

• No. of families

12 5 22

Fig1-A. Breeding Program of Salt Tolerance Rice improvement 20032008

Fig1-B. Breeding Program of Salt Tolerance Rice improvement 20032008

Evaluation for Yield Performance Test in Target Location GCP&BIOTEC MAS

Year ×

BC3F4 IR53936 BC3F4(77plts ) BC3F3(245lines) MAS 2006WS 2007DS 2007WS 2007WS 2008DS 2008WS

• No. of families

17 17 10 8 , 42 BC4F1 BC4F2 MAS BC3F4 BC3F5

Results and Discussion

BC4F2 and BC3F5 population of IR53936/Pokkali with SalT Marker

RM3412

RM1287

RM10772

RM411

RM457

RM5349

Table 1. Observation of salt tolerant plants on Marker Assisted Selection (2008 May) Marker Homo IR Homo PK Hetero Off- type Missing Total SalT 126 321 119 15 582 RM 10720 (Monomorphism) 282

• 282

RM3412 92 403 39 34 14 582 RM411 293 10 303 RM1287 1 302 303 RM10772 303 303

• Table2. . Observation of quality traits on salt tolerant

introgression lines (2008 May) Marker Total tested samples Homo IR Homo PK Hetero Total Waxy 303 303 303 RM5349 303 303 303 RM457 303 303 303 RM21 303 303 303

Table 3.Selected salt tolerant plants by MAS (2008 May)

Generation

• No. of

Progenies Tested plants Selected Progenies Selected plants BC4F2 8 170 6 60 BC3F5 42 412 34 243 Total 50 582 40 303

Discussion

• According to the PCR results of SalT marker, 321

homozygous plants with Pokkali gene were selected from 582 sample plants of BC4F2 and BC3F5 populations.

• All of samples were observed in monomorphisms like

IR53936 on result of Marker RM10720.

• RM3412 Marker helped to select 403 homozygous

plants with Pokkali gene.

• 303 plants of homozygous Pokkali gene from results of

both SalT and RM3412 were selected to continue for the other Markers.

Discussion

• Only 10 plants were identified again from selected salt

tolerant homozygous with Pokkali by use of RM411 Marker.

• RM1287 and RM10772 Markers proved that 303

homozygous plants had salt tolerance like Pokkali.

• Quality traits on homozygous salt tolerant lines were

evaluated by use of different markers such as Waxy, RM5349,RM457and RM21.

• According to these results ,all 303 samples were the

same as parent IR53936 on Amylose content and Gel Temperature.

• That’s why these selected lines were expected to have

good eating quality with salt tolerant ability.

Future Program

• 1. Screening test for selected 303 lines by MAS

To use Yoshida solution culture To test in concrete pond with salty soil

• 2. Evaluation for yield Performance Test

Test Entries------60 (selected lines by MAS) Year/Season------2008 wet season Locations--------- 2 (kyaukse and Myaungmya Rice Research Farms)

Design-------------Augmented design

Plot size------------5 m x 0.8 m2 (5meter×4rows) Spacing ----------- 20 x 20cm Number of seeding/hill -------single Checkes ----------- IR53936 and Pokkali Local checkes ---- Manawthukha and Sangankhan.4.

Records

• 1. Yield Component and Agronomic Characters

50% Flowering days Plant Height (cm) Number of productive tillers/hill Number of total grains/panicle Number of filled grains/ panicle Number of unfilled grains/ panicle 1000 grain wt(gm) Pest and Disease Phenotypic acceptability at maturity Yield/Plot (gm)

Records

• 2. Grain Quality Analysis

Amylose Content (%) Gel Consistency Gel Temp: Grain L/B ratio Kernal Appearance Milling recovery(%) Eating quality

• 3. Score levels will be determined by use of IRRI SES