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The community of practices

“concept applied to rice production in the Mekong Region:Quick conversion of popular rice varieties with emphasis on drought, salinity and grain quality improvement “

Report

Improvement of TDK 1 for Aroma and Cooking Quality Traits using Marker-assisted Selection

Mr Souvanh Thadavong and Mr Khemkham Hongphakdy Agricultural Research Center (ARC), National Agriculture and Forestry Research Institute (NAFRI), Ministry of Agriculture and Forestry, Vientiane, Lao PDR.

Introduction

• Rice is a staple food in the Lao PDR.
• Approximately 95% of population consume glutinous rice.
• Thadokkham 1 (TDK 1) is one glutinous improved variety which

was released by National Rice Research Program since 1993.

• Up to now TDK1 grow in a large areas
• This variety give high yielding and good eating, but not aroma,

therefore TDK 1 need to be improved for aroma trait.

Objective

This study aim to improve TDK 1 with aroma trait by using Marker Assist Selection.

Plant material

The marker assisted backcross breeding is conducted using TDK1 which is popular and grow in large area of the country, use as a recipient and local variety (Homnangnouane) with aroma, use as a donor Recipient: TDK 1 (Glutinous rice) Donor: Homnangnouane ( Glutinous and aroma rice)

From BC2F2 we produced 6 BC3F1 plants in NAFRI and 94 plants BC3F1. The whole of BC3F1 plant were bring to MAS workshop for select heterozygous trait using marker-assisted selection.

Plant material (Cont.)

Figure 1: Diagram of marker-assisted backcross breeding of TDK1

BC2F2 BC3F1 MAS x TDK1 BC3F1 x TDK1+Sub1

Parental Polymorphism Survey

BADH marker were used for parental polymorphism survey

Methodology

• 1. DNA extraction
• 2. DNA concentration
• 3.PCR applification
• 4. Lines selection

Every step follow LAB MANUAL of this workshop.

Marker Assisted Selection for F1

The Methodology: DNA extraction, DNA concentration, PCR amplification and Lines selection The objective of F1 screening is focused on selected plants which carried heterozygous alleles of Homnangnouane

DNA of F1 progenies concentration

The polyacrylamide gel of F1 genotyping with BADH

Marker Assisted Selection for F1

Among 96 selected plants of F1, 45 plants carried heterozygous

• f F1 plants

No DNA sample Marker BADH No DNA sample Marker BADH 1 3 2 15 19 2 2 4 2 16 20 2 3 6 2 17 21 2 4 7 2 18 23 2 5 8 2 19 24 2 6 9 2 20 25 2 7 11 2 21 27 2 8 12 2 22 28 2 9 13 2 23 32 2

No DNA sample Marker BADH 29 59 2 30 71 2 31 72 2 32 73 2 33 74 2 34 75 2 35 76 2 36 77 2 37 78 2 38 82 2 39 83 2 40 84 2 41 85 2 42 89 2 43 90 2 44 92 2 45 93 2

Conclusion

• The study aims to improve TDK1 with aroma and good eating quality

by MAS.

• From 96 plants of F1, we got only 45 plants of heterozygous of Homnangnouane.
• Ten plants selected from 45 plants of heterozygous of Homnangnouane will be

continue to crossing with TDK1+Sub1.

• In addition, we learn more about Waxy, PB7-8( bacterial leaf blight), SNPs 2341

( Single Nuclear Type Polymorphism), Gelatinization Temperature (GT), Gel Consistency (GC), Amylose Content (AC) and understand that the MAS method is very helpful, exactly identify and quick technique for breeding program.

Acknowledgement

We would like to thank to sponsor’s projects:

• the Generation Challenge Program (GCP)
• BIOTEC
• Kasetsart University

Especially, we would like to thank: Dr. Apichart Vanavichit and Dr. Teerayut Toojinda, who give opportunity to participate in this course, advice technology of Bio-Technology and transfer knowledge for us. Finally, we would like to thank all teachers and all staffs in Rice Gene Discovery Unit to provide and help during the work shops.