SLIDE 1
Photothermal therapy Drug MRI contrast agents Photothermally controlled drug delivery Au layer
Multifunctional Nanoparticle Multifunctional Nanoparticle
Biocompatible & biodegradable polymer Magnetic layer
Kyung-Hwa Yoo (Yonsei University) Multifunctional Nanoparticle - - PowerPoint PPT Presentation
Kyung-Hwa Yoo (Yonsei University) Multifunctional Nanoparticle - - PowerPoint PPT Presentation
Multifunctional Nanoparticles for Photothermally Controlled Drug Delivery and Magnetic Resonance Imaging Enhancement Kyung-Hwa Yoo (Yonsei University) Multifunctional Nanoparticle Multifunctional Nanoparticle Photothermal therapy Au layer
SLIDE 2
SLIDE 3
Si substrate
PMMA
Si substrate
PMMA
Si substrate
PMMA
O2 plasma PS beads deposition Au deposition Lift-off PLGA beads
Au nanoshell
SLIDE 4
Wavelength (nm) Absorbance (a.u.)
400 600 800 1000 1200 0.5 1.0 15 nm 20 nm 25 nm
400 600 800 1000 1200
Wavelength (nm)
(b) PS 10 nm 14 nm 20 nm 30 nm
Visible/NIR spectra Visible/NIR spectra
SLIDE 5
50 100 150 200 36 38 40 42 44 46
Temperature (
- C)
Time (s)
PS-Au half-shell Au nanoparticle Water PS nanoparticle
Hyperthermia
SLIDE 6
50 100 150 200 30 35 40 45
P=7 W/cm
2
C=145 µg/ml
P=15 W/cm
2
P=25 W/cm
2
50 100 150 200 30 35 40 45
36.25 µg/mL 72.5 µg/mL
P=25 W/cm
2
145 µg/mL
Time (s) Time (s) (a) (b)
Hyperthermia
SLIDE 7
Photothermal toxicity Photothermal toxicity (laser 15a) (laser 15a) Without particle Without particle Laser Laser 15a 50 min 15a 50 min Calcein-AM Calcein-AM Bright field image Bright field image With particle With particle incubation incubation (100ug/ml) 1 day (100ug/ml) 1 day Laser Laser 15a 15 min 15a 15 min 50 min 50 min
SLIDE 8
1 2 3 4 20 40 60 80 100
with NIR irradiation without NIR irradation slope = 9.0738 day
- 1
slope = 18.059 day
- 1
Cumulative % Released Time (day) (a)
Drug Release with & without NIR irradiation Drug Release with & without NIR irradiation
SLIDE 9
0 min. 15 min. 50 min. 2 μm 2 μm 2 μm 0 min. 15 min. 50 min. 2 μm 2 μm 2 μm (b) With NIR illumination (b) Without NIR illumination
SLIDE 10
0 min. 10 min. 15 min. 50 min. 0 min. 10 min. 15 min. 50 min.
300 nm 300 nm
(a) (b)
TIRFM images TIRFM images
SLIDE 11
0 day After 2-day NIR irradiation After 3-day NIR irradiation After 3 days without NIR irradiation
SLIDE 12
1 2 3 30 40 50 60
700 800 900 0.36 0.38 0.40
0 day 1 day 2 day 3 day 7 day
5 10 15 20 20 25 30 35 40 45 50 55 60
Temperature (
- C)
Time (min)
(a) (b) Wavelength (nm) Time (min.) Absorbance (a.u.) Temperature (°C)
SLIDE 13
Absorbance (a.u.) Wavelength (nm) Relative nanoparticle concentration 1/T2 (1/sec.)
0.0 0.5 1.0 1.5 2 4
Mn(10 nm)/Au(25 nm) H-S NPs Mn(0 nm)/Au(25 nm) H-S NPs
(a) (b) (c)
Mn Au 400 600 800 1000 0.28 0.32 0.36 Mn (10 nm)/Au (0 nm) Mn (10 nm)/Au (25 nm)
SLIDE 14