Kenneth Dawson Centre for BioNano Interactions EMEA ITF Group on - - PowerPoint PPT Presentation

Kenneth Dawson Centre for BioNano Interactions

EMEA ITF Group on Nanotechnologies April 29th 2009 Website: www.cbni.eu

BioNanoInteractions

Area(s) of expertise

Nanoparticle synthesis Protein Corona Transcriptomics and Proteomics Disease / therapy In vivo effects Intracellular Visualization Dispersion and characterisation

GLP

Class III nanoparticle synthesis Class III Protein Expression Class III Cell Culture

New Institute,Integrated Nano And Biology, Good Laboratory Practice (?)

160-200 people

Protein Corona paradigm

Nanoparticles utilise existing biological pathways (in new ways?)

Uptake connected to protein corona

Nanotechnology for the Pharmaceutical industry

1 2 6 3 4 5 K15= [5] [1]

50 100 150 200 250 50 100 150 200 250 E xpos ure time, minutes FITC log 37C 4C NaN3

1 2 3 6 5 4

5 10 15 20 25 30 50 100 150 200 250 300 E xpos ure time, minutes F IT C lo g 37C 4C N aN 3

Completely different uptake behaviour

30 60 90 120 150 180 210 240 5 10 15 20 25 30 35

Cell Fluorescence Intensity Exposure time, minutes

37C 4C NaN3

Distinct localisation in ER Clear energy dependence

Quality And Standards Issues

Sds-gel (4% stacking gel and 10% resolving gel)

particles Free dye (<6 kda)

Much of the literature in this area questionable! Also observed for FITC-dextrans etc., not only nanoparticles. Commercial samples leak - free dye!

new methods in-situ characterization

NanoSight Spinning Disc Centrifugation

SiO2 nanoparticles with increasing plasma concentration

Rel Weight

NIST Gold nanoparticles 30nm and 60nm in citrate buffer